bacterial transformation kit life-technologies - (Jan/10/2014 )
Hi you guys,
I did my transformation yesterday. I supposed that pUC19 supplied in the kit is for the positive control.
Strikingly, I had few (around 50 colonies) in this positive control (pUC19) but a several hundreds with my plasmid (after gateway LR reaction).
Do you have any explanation for my case?
Thanks in advance.
Trang
-trangtrang-
Hi Trang. Unless your plasmid was also pUC19 and you used the exact same amount, then you are not really looking at a comparable control. Colony counts can be interesting to do, but they rarely provide any useful information. The results from a screen (either digest or PCR) of colonies on your experimental plate are more important.
-dimensionsbio-