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Top : New Forum Archives (2009-): : Molecular-Biology
481. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
482. SAP dephosphorylation - (reply: 1)
483. Determining in frame in modified vector - (reply: 1)
484. Deleting restriction enzyme sites - (reply: 1)
485. Creating primers to add restriction sites to vector backbone - (reply: 7)
486. cDNA test on 1% agarose gel - (reply: 3)
487. Plasmid isolation from gram positive bacteria - (reply: 2)
488. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
489. rsaI enzyme - (reply: 1)
490. How to check for the presence of plasmid? - (reply: 3)
491. Extracting total RNA from woody plant roots - (reply: 2)
492. Quantify total DNA in small cell lysate...? - (reply: 1)
493. Quantify DNA in gel electrophoresis bands - (reply: 7)
494. Protein extraction and isolectric focusing - (reply: 1)
495. getting strange data in Luciferase-renilla assay - (reply: 2)
496. Homozygote DNA but Heterozygote RNA? - (reply: 2)
497. sacB Counter-Selection to generate double cross-over deletion mutants - (reply: 8)
498. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
499. What is the necessary time for lipofectamin to make complex with pre-miRNA oligo - (reply: 1)
500. what is the ORFeome clone? - (reply: 2)
501. ligation with one ristrection site/enzyme - (reply: 3)
502. No amplification during RT-PCR - (reply: 4)
503. rescue cloning of a transposon mutant - (reply: 2)
504. Dephosphorylation - (reply: 7)
505. study of receptors in cell lines - (reply: 1)
506. Mouse genotyping issues - (reply: 1)
507. In-situ hybridization - (reply: 1)
508. His tag introduction in to gene and primer design - (reply: 2)
509. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
510. Principle of silica membrane binding of nucleic acid - (reply: 6)