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Top : New Forum Archives (2009-): : Molecular-Biology
481. Creating primers to add restriction sites to vector backbone - (reply: 7)
482. cDNA test on 1% agarose gel - (reply: 3)
483. Plasmid isolation from gram positive bacteria - (reply: 2)
484. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
485. rsaI enzyme - (reply: 1)
486. How to check for the presence of plasmid? - (reply: 3)
487. Extracting total RNA from woody plant roots - (reply: 2)
488. Quantify total DNA in small cell lysate...? - (reply: 1)
489. Quantify DNA in gel electrophoresis bands - (reply: 7)
490. Protein extraction and isolectric focusing - (reply: 1)
491. getting strange data in Luciferase-renilla assay - (reply: 2)
492. Homozygote DNA but Heterozygote RNA? - (reply: 2)
493. sacB Counter-Selection to generate double cross-over deletion mutants - (reply: 8)
494. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
495. What is the necessary time for lipofectamin to make complex with pre-miRNA oligo - (reply: 1)
496. what is the ORFeome clone? - (reply: 2)
497. ligation with one ristrection site/enzyme - (reply: 3)
498. No amplification during RT-PCR - (reply: 4)
499. rescue cloning of a transposon mutant - (reply: 2)
500. Dephosphorylation - (reply: 7)
501. study of receptors in cell lines - (reply: 1)
502. Mouse genotyping issues - (reply: 1)
503. In-situ hybridization - (reply: 1)
504. His tag introduction in to gene and primer design - (reply: 2)
505. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
506. Principle of silica membrane binding of nucleic acid - (reply: 6)
507. genogroup and genotype - (reply: 2)
508. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
509. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
510. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)