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991. Internal Control RNA or DNA - (reply: 4)
992. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
993. RNA extraction - (reply: 3)
994. mutation using overlapping PCR, what's the success rate? - (reply: 3)
995. finding exons for a gene, having the coding sequence only - (reply: 4)
996. colonies without insert!!! - molecular biology (reply: 1)
997. Effect of phosphoric acid on a kinase assay - (reply: 2)
998. Proteinase K stock solution - (reply: 3)
999. Total RNA only produce 1 band in gel - (reply: 2)
1000. Advice on amount of starting template for subcloning - (reply: 3)
1001. New to Gene cloning - Plasmid vector preparation (reply: 2)
1002. PCR for MULTIPLE mutagenesis - (reply: 4)
1003. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
1004. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
1005. Induction strength : IPTG vs lactose - which one is stronger? (reply: 2)
1006. EMSA - probe disappears with nuclear extract - probe works without nuclear extract... (reply: 1)
1007. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
1008. Digested PCR product migrate slower than uncut - (reply: 8)
1009. PCR question - (reply: 2)
1010. cloning a 34kb insert - please suggest good vectors (reply: 1)
1011. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
1012. Do you know good sequencing companies? - Sanger sequencing (reply: 15)
1013. RNA extraction troubleshooting: low 260/230 - (reply: 1)
1014. Illumina sample prep for Multiplexed sequencing... - How does it work? (reply: 3)
1015. RNA in native gel - (reply: 3)
1016. Comment of this cloning strategy - (reply: 3)
1017. Problems with transfection in human cells - (reply: 3)
1018. RNA extraction from FTA classic - (reply: 1)
1019. mCherry propagation - (reply: 1)
1020. ClustalW question - on multiple alignment of DNA sequences (reply: 1)
992. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
993. RNA extraction - (reply: 3)
994. mutation using overlapping PCR, what's the success rate? - (reply: 3)
995. finding exons for a gene, having the coding sequence only - (reply: 4)
996. colonies without insert!!! - molecular biology (reply: 1)
997. Effect of phosphoric acid on a kinase assay - (reply: 2)
998. Proteinase K stock solution - (reply: 3)
999. Total RNA only produce 1 band in gel - (reply: 2)
1000. Advice on amount of starting template for subcloning - (reply: 3)
1001. New to Gene cloning - Plasmid vector preparation (reply: 2)
1002. PCR for MULTIPLE mutagenesis - (reply: 4)
1003. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
1004. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
1005. Induction strength : IPTG vs lactose - which one is stronger? (reply: 2)
1006. EMSA - probe disappears with nuclear extract - probe works without nuclear extract... (reply: 1)
1007. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
1008. Digested PCR product migrate slower than uncut - (reply: 8)
1009. PCR question - (reply: 2)
1010. cloning a 34kb insert - please suggest good vectors (reply: 1)
1011. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
1012. Do you know good sequencing companies? - Sanger sequencing (reply: 15)
1013. RNA extraction troubleshooting: low 260/230 - (reply: 1)
1014. Illumina sample prep for Multiplexed sequencing... - How does it work? (reply: 3)
1015. RNA in native gel - (reply: 3)
1016. Comment of this cloning strategy - (reply: 3)
1017. Problems with transfection in human cells - (reply: 3)
1018. RNA extraction from FTA classic - (reply: 1)
1019. mCherry propagation - (reply: 1)
1020. ClustalW question - on multiple alignment of DNA sequences (reply: 1)