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991. Protein in the nucleus vs in the cytoplasm - (reply: 1)
992. dsRNA detection in vivo - (reply: 3)
993. Designing primer to remove his-tag - (reply: 1)
994. SSRs; STRs and microsatellites - What is the difference? (reply: 5)
995. problem in protein purification - (reply: 5)
996. Retroviral transduction of T cells - (reply: 4)
997. strange problem in PAGE gel - (reply: 12)
998. DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
999. Gene targeting - (reply: 3)
1000. PCR product too short - (reply: 6)
1001. Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
1002. When do I add selection? - (reply: 2)
1003. How to prevent concatemerization during ligation reaction? - (reply: 2)
1004. Washing RNA pellet - 85% ethanol? - (reply: 2)
1005. Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
1006. Unsuccessful cloning with one sticky end & one blunt end - Low transformation efficiency & colonies do not contain insert (reply: 8)
1007. White colonies no insert - (reply: 2)
1008. any strategy to insert MCS into a specific region without any restriction sites - (reply: 5)
1009. Isolating bacterial RNA and protein from the host tissue sample - (reply: 3)
1010. DOT BLOT for pcr product - No result obtain (reply: 1)
1011. Backwards electrophoresis electrodes - Can you dig it? (reply: 1)
1012. Do plasmids work in bacteria? - (reply: 1)
1013. Smears above ribosomal RNA bands - RNA extraction from microalgae (reply: 2)
1014. CEN sequences on plasmids (yeast) - (reply: 4)
1015. DMSO - (reply: 1)
1016. Trizol Extraction of PBMCs, low 260/230 - (reply: 5)
1017. PCR amplification with Pfu / quality of DNA - (reply: 4)
1018. primer design - (reply: 2)
1019. recombinant ecoli in log phase overnight media suggestion - (reply: 2)
1020. TRIzol phase extraction with Lock-Phase tubes - Does anyone use Lock-Phase? (reply: 1)
992. dsRNA detection in vivo - (reply: 3)
993. Designing primer to remove his-tag - (reply: 1)
994. SSRs; STRs and microsatellites - What is the difference? (reply: 5)
995. problem in protein purification - (reply: 5)
996. Retroviral transduction of T cells - (reply: 4)
997. strange problem in PAGE gel - (reply: 12)
998. DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
999. Gene targeting - (reply: 3)
1000. PCR product too short - (reply: 6)
1001. Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
1002. When do I add selection? - (reply: 2)
1003. How to prevent concatemerization during ligation reaction? - (reply: 2)
1004. Washing RNA pellet - 85% ethanol? - (reply: 2)
1005. Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
1006. Unsuccessful cloning with one sticky end & one blunt end - Low transformation efficiency & colonies do not contain insert (reply: 8)
1007. White colonies no insert - (reply: 2)
1008. any strategy to insert MCS into a specific region without any restriction sites - (reply: 5)
1009. Isolating bacterial RNA and protein from the host tissue sample - (reply: 3)
1010. DOT BLOT for pcr product - No result obtain (reply: 1)
1011. Backwards electrophoresis electrodes - Can you dig it? (reply: 1)
1012. Do plasmids work in bacteria? - (reply: 1)
1013. Smears above ribosomal RNA bands - RNA extraction from microalgae (reply: 2)
1014. CEN sequences on plasmids (yeast) - (reply: 4)
1015. DMSO - (reply: 1)
1016. Trizol Extraction of PBMCs, low 260/230 - (reply: 5)
1017. PCR amplification with Pfu / quality of DNA - (reply: 4)
1018. primer design - (reply: 2)
1019. recombinant ecoli in log phase overnight media suggestion - (reply: 2)
1020. TRIzol phase extraction with Lock-Phase tubes - Does anyone use Lock-Phase? (reply: 1)