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991. How to remove the background and unspecific bands - (reply: 12)
992. Low Miniprep Yield - (reply: 1)
993. Primer design - (reply: 3)
994. Problems for saturation mutagenesis library - (reply: 12)
995. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
996. RNA from guinea pig lung=degraded - (reply: 5)
997. What transcription factors are involved in transient transfection - (reply: 1)
998. Tissue disruption - for RNA isolation (reply: 2)
999. concentration - concentration of DNA (reply: 3)
1000. Random digest of double stranded DNA - (reply: 1)
1001. Best transcription vector - (reply: 1)
1002. Problem in picking single colony - (reply: 2)
1003. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
1004. Agarose - (reply: 7)
1005. RNA - extraction and pcr (reply: 1)
1006. Problem with maxiprep - (reply: 2)
1007. selecting drug-resistant colon cancer cells - (reply: 2)
1008. increased mRNA transcript but no activity - (reply: 5)
1009. Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
1010. Gene expression change after mutagenesis-- please help! - (reply: 4)
1011. Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
1012. real-time PCR after restriction enzyme digestion - (reply: 6)
1013. DH5alpha cells - (reply: 4)
1014. DNA extraction of microalgae - microalgae (reply: 3)
1015. gel question - (reply: 3)
1016. Chemiluminescent EMSA - How to develop X-ray film (reply: 3)
1017. EMSA - Digoxygenin labelled kit (reply: 2)
1018. Acquiring cricket paralysis virus - Where can I find some? (reply: 1)
1019. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
1020. DNA probe labeling for EMSA; How? - Long probe, not oligonucletides (reply: 1)
992. Low Miniprep Yield - (reply: 1)
993. Primer design - (reply: 3)
994. Problems for saturation mutagenesis library - (reply: 12)
995. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
996. RNA from guinea pig lung=degraded - (reply: 5)
997. What transcription factors are involved in transient transfection - (reply: 1)
998. Tissue disruption - for RNA isolation (reply: 2)
999. concentration - concentration of DNA (reply: 3)
1000. Random digest of double stranded DNA - (reply: 1)
1001. Best transcription vector - (reply: 1)
1002. Problem in picking single colony - (reply: 2)
1003. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
1004. Agarose - (reply: 7)
1005. RNA - extraction and pcr (reply: 1)
1006. Problem with maxiprep - (reply: 2)
1007. selecting drug-resistant colon cancer cells - (reply: 2)
1008. increased mRNA transcript but no activity - (reply: 5)
1009. Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
1010. Gene expression change after mutagenesis-- please help! - (reply: 4)
1011. Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
1012. real-time PCR after restriction enzyme digestion - (reply: 6)
1013. DH5alpha cells - (reply: 4)
1014. DNA extraction of microalgae - microalgae (reply: 3)
1015. gel question - (reply: 3)
1016. Chemiluminescent EMSA - How to develop X-ray film (reply: 3)
1017. EMSA - Digoxygenin labelled kit (reply: 2)
1018. Acquiring cricket paralysis virus - Where can I find some? (reply: 1)
1019. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
1020. DNA probe labeling for EMSA; How? - Long probe, not oligonucletides (reply: 1)