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991. Plasmid, gene orientation, insert orientation. - (reply: 1)
992. Need stable GFP expression in IPS and embryonic SCs - (reply: 1)
993. good tutorials/protocols/books on gene cloning - (reply: 2)
994. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
995. yeast growth in SC-ura medium - (reply: 1)
996. Optimizing Trizol mRNA extraction - (reply: 3)
997. Expression in trans - (reply: 1)
998. DNA Storage - (reply: 10)
999. RBC LYSIS BUFFER - a good substitute of sucrose? - (reply: 5)
1000. Luciferase assay - expected range of raw reading values - (reply: 1)
1001. compare between 2 or more genes - (reply: 1)
1002. sample preservation for RNA extraction - (reply: 1)
1003. Designing primers for the ORFs - (reply: 9)
1004. Bioinformatics Software - (reply: 2)
1005. Low yields with quiagen midi kit - (reply: 2)
1006. RNA extraction from blood stain - (reply: 1)
1007. Knocking out non-essential gene - (reply: 2)
1008. PCR with phophorylated primers - (reply: 1)
1009. Nanodrop absorbtion at 290 nm??? - (reply: 1)
1010. Plasmid sequencing without primers - (reply: 1)
1011. Either too low or too high a260/a230 - (reply: 5)
1012. RNA purity - (reply: 3)
1013. RNA Buffer Extraction - (reply: 3)
1014. wet chamber for ISH - (reply: 4)
1015. Convertion of 100% glycerol to 80% - (reply: 1)
1016. Difference between EST and cDNA? - (reply: 4)
1017. question related to mutagenesis - (reply: 1)
1018. Genotyping by allele specific PCR - (reply: 6)
1019. Size and composition of spacer between protein and tag? - (reply: 4)
1020. Wacky BioAnalyzer Results - (reply: 12)
992. Need stable GFP expression in IPS and embryonic SCs - (reply: 1)
993. good tutorials/protocols/books on gene cloning - (reply: 2)
994. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
995. yeast growth in SC-ura medium - (reply: 1)
996. Optimizing Trizol mRNA extraction - (reply: 3)
997. Expression in trans - (reply: 1)
998. DNA Storage - (reply: 10)
999. RBC LYSIS BUFFER - a good substitute of sucrose? - (reply: 5)
1000. Luciferase assay - expected range of raw reading values - (reply: 1)
1001. compare between 2 or more genes - (reply: 1)
1002. sample preservation for RNA extraction - (reply: 1)
1003. Designing primers for the ORFs - (reply: 9)
1004. Bioinformatics Software - (reply: 2)
1005. Low yields with quiagen midi kit - (reply: 2)
1006. RNA extraction from blood stain - (reply: 1)
1007. Knocking out non-essential gene - (reply: 2)
1008. PCR with phophorylated primers - (reply: 1)
1009. Nanodrop absorbtion at 290 nm??? - (reply: 1)
1010. Plasmid sequencing without primers - (reply: 1)
1011. Either too low or too high a260/a230 - (reply: 5)
1012. RNA purity - (reply: 3)
1013. RNA Buffer Extraction - (reply: 3)
1014. wet chamber for ISH - (reply: 4)
1015. Convertion of 100% glycerol to 80% - (reply: 1)
1016. Difference between EST and cDNA? - (reply: 4)
1017. question related to mutagenesis - (reply: 1)
1018. Genotyping by allele specific PCR - (reply: 6)
1019. Size and composition of spacer between protein and tag? - (reply: 4)
1020. Wacky BioAnalyzer Results - (reply: 12)