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Top : New Forum Archives (2009-): : Molecular-Biology
1861. DNA purification - DNA purification dissolved in water (reply: 3)
1862. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
1863. PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
1864. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
1865. Double digestion using EcoRI and HindIII with pHAT-CAT - (reply: 4)
1866. dh5 alpha competent cells? - (reply: 2)
1867. Hypoxia - downregulated HIF-1A - why me??? (reply: 2)
1868. Bioanalyzer not recognising the chip - chip misaligned/manufacturing problem? - (reply: 4)
1869. transient transfection - (reply: 1)
1870. Bad sequence - (reply: 5)
1871. Southern Blot troubleshooting - Please help (reply: 3)
1872. Removing sequencing migration artefacts - (reply: 1)
1873. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
1874. Probes design for southern-blot - need more information for probes design for southern-blot (reply: 3)
1875. finding promoter - (reply: 1)
1876. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
1877. Normalizing RNA concentrations prior to cDNA production - qPCR and making cDNA (reply: 1)
1878. precipitation of secreted protein from growth media - (reply: 1)
1879. EMSA gel problem - (reply: 2)
1880. cloning - (reply: 6)
1881. Generating stable cell line - (reply: 2)
1882. Can't get clean RNA prep with TRIzol - (reply: 2)
1883. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
1884. vectors maps - (reply: 2)
1885. Fusion of reporter genes - (reply: 2)
1886. reuse electroporation cuvette? - (reply: 4)
1887. PCR Master mix - (reply: 1)
1888. Fake positive colony in lamda RED reconbination, why? - (reply: 2)
1889. Cloning from cDNA - the wrong gene?! - (reply: 2)
1890. Ligation - (reply: 3)