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Top : New Forum Archives (2009-): : Molecular-Biology
1861. extraction of viral rna from plant tissue - (reply: 1)
1862. ampicillin for LB agar - (reply: 2)
1863. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
1864. PCR not working - overhangs too long? (reply: 3)
1865. What melting temperature to use - (reply: 1)
1866. PFGE Current? - (reply: 1)
1867. Plasmid frameshift problem after clonning - (reply: 2)
1868. Nde1-BamH1 makes me sad - high background despite DIFFERENT methods (reply: 34)
1869. TA cloning necessary? - (reply: 2)
1870. Is DNA an acid because of phosphate group - (reply: 2)
1871. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
1872. colony formation assay - how many cells to seed (reply: 2)
1873. Conversion of primer unit - ug/ul to uM (reply: 4)
1874. mutagenesis by PCR or just use a kit - (reply: 5)
1875. Drying RNA in Speedvac - (reply: 1)
1876. cDNA for qPCR - Help with basics please (reply: 6)
1877. plasmid extraction kit solution composition - wash buffer (reply: 2)
1878. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
1879. Primer Trouble Shooting - (reply: 5)
1880. Proven Primers Stopped working & now leave smear! - (reply: 2)
1881. Expired chemicals - 0.5M EDTA, can be used again after autoclaved? - (reply: 4)
1882. tri reagent not compatible with plastic? - (reply: 1)
1883. DNA Extraction - (reply: 2)
1884. ligation problem - (reply: 6)
1885. TRIzol v. TRI reagent - (reply: 2)
1886. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
1887. Isolation of DNA from Scat - (reply: 2)
1888. How long will cellular resources last - (reply: 1)
1889. PFGE conditions - (reply: 2)
1890. genomic DNA isolation - (reply: 4)