Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1231. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
1232. Adapter ligation problem - (reply: 5)
1233. Primer design with a tag - (reply: 4)
1234. Lithium electrophoresis buffer - Why does it not work? (reply: 2)
1235. Weird migration of double digested plasmid - (reply: 4)
1236. Run agarose/formaldehyde gel for 5 mins before loading samples? Why? - (reply: 1)
1237. Myc tags - (reply: 1)
1238. MOPS: How do you sterlise yours? - (reply: 2)
1239. Which gel concentration - (reply: 4)
1240. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
1241. PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
1242. MINI Dialysis Units - below 2K MWCO? (reply: 1)
1243. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
1244. Rotten Fish Smell in TAE Buffer - Stinky TAE (reply: 3)
1245. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
1246. Combining forward and reverse primer gives different size on gel - (reply: 2)
1247. Help for co-transfection of proteins and plasmid - (reply: 1)
1248. PCR of complement genomic DNA - (reply: 3)
1249. RNAi is somatic and germline cells - (reply: 3)
1250. subcloning into bacterial expression vector - (reply: 1)
1251. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
1252. cloning into F1 ori of expression vector??? - (reply: 1)
1253. primers for fungal identification - (reply: 9)
1254. protein overexpression in HeLa cells - (reply: 1)
1255. TBE Acrylamide/Formamide gels running slowly - (reply: 4)
1256. Sequencing promoter regions - (reply: 6)
1257. PCR for long and repetitive region from genomic DNA - (reply: 4)
1258. dna isolation - dna isolation (reply: 1)
1259. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
1260. DNA extraction from plated colonies - (reply: 3)