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Top : New Forum Archives (2009-): : Molecular-Biology
1591. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1592. grow in broth but not on agar... - after transformation (reply: 2)
1593. How many colonies to screen (colony PCR)? - (reply: 4)
1594. Primer Design - aligning sequences for probe/primer design (reply: 2)
1595. Cloning problem - (reply: 2)
1596. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
1597. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1598. In situ hybridization enigma - (reply: 1)
1599. A cloning nightmare - (reply: 8)
1600. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1601. RNA fragmentation - (reply: 3)
1602. RNA extraction - how many cells do I need at least?? (reply: 4)
1603. RNA extraction protocol for small amounts of cells - (reply: 2)
1604. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
1605. DNA sample contaminated with lipids - (reply: 8)
1606. cloning problem - (reply: 5)
1607. colony PCR inconsistent - (reply: 4)
1608. Newbie to Sequencing Question - (reply: 1)
1609. Problem with gel extraction of DNA. - (reply: 1)
1610. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
1611. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
1612. genomic DNA isolation - (reply: 1)
1613. E. coli not growing in liquid cultures :( - (reply: 4)
1614. Tricine SDS-PAGE for plant mitochondria - Membrane proteomics (reply: 1)
1615. DNA product almost 1500 bp - (reply: 1)
1616. Slightly too high 260:280 and 260:230 ratios from gDNA extraction - Should I care? (reply: 2)
1617. Blunt end filling - Blunt end filling (reply: 1)
1618. GHOST in Electrophoresis - (reply: 18)
1619. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
1620. Transformation background problem - high background in negative control (reply: 9)