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1591. How to obatin intron sequence from cDNA sequence? - (reply: 1)
1592. genomic DNA extraction - why there appears a smear on the gel photo (reply: 11)
1593. Colony Pcr - primers - (reply: 5)
1594. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
1595. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
1596. Sequence size and insert size - Sequence size has larger base pair than insert size (reply: 2)
1597. QCMD samples - (reply: 5)
1598. competent cells - OD600 reading (reply: 6)
1599. Creating overhangs with PCR - (reply: 4)
1600. RNA: Is this degratation or something else? - (reply: 1)
1601. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1602. Is there any software that can be sued to design degenerate primers - (reply: 1)
1603. Primer Decontamination - (reply: 2)
1604. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1605. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1606. How long is "overnight"? - (reply: 3)
1607. Trizol stability - (reply: 1)
1608. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1609. Tube Openers - (reply: 3)
1610. taq man probes non specific? - (reply: 1)
1611. Help! low efficiency of transfection - (reply: 2)
1612. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1613. Cloning before sequencing- is it necessary??? - (reply: 13)
1614. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1615. Guanadine HCl for plasmid miniprep - (reply: 3)
1616. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1617. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1618. 3īRACE problem - only smear - (reply: 4)
1619. salt for DNA precipitation - DNA purification (reply: 3)
1620. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1592. genomic DNA extraction - why there appears a smear on the gel photo (reply: 11)
1593. Colony Pcr - primers - (reply: 5)
1594. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
1595. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
1596. Sequence size and insert size - Sequence size has larger base pair than insert size (reply: 2)
1597. QCMD samples - (reply: 5)
1598. competent cells - OD600 reading (reply: 6)
1599. Creating overhangs with PCR - (reply: 4)
1600. RNA: Is this degratation or something else? - (reply: 1)
1601. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1602. Is there any software that can be sued to design degenerate primers - (reply: 1)
1603. Primer Decontamination - (reply: 2)
1604. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1605. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1606. How long is "overnight"? - (reply: 3)
1607. Trizol stability - (reply: 1)
1608. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1609. Tube Openers - (reply: 3)
1610. taq man probes non specific? - (reply: 1)
1611. Help! low efficiency of transfection - (reply: 2)
1612. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1613. Cloning before sequencing- is it necessary??? - (reply: 13)
1614. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1615. Guanadine HCl for plasmid miniprep - (reply: 3)
1616. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1617. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1618. 3īRACE problem - only smear - (reply: 4)
1619. salt for DNA precipitation - DNA purification (reply: 3)
1620. Conditional knockout mated to cre not deleting target exon - (reply: 8)