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1591. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1592. 3īRACE problem - only smear - (reply: 4)
1593. salt for DNA precipitation - DNA purification (reply: 3)
1594. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1595. Not getting plasmid from miniprep - (reply: 7)
1596. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1597. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1598. Is this consider smearing? - (reply: 2)
1599. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1600. grow in broth but not on agar... - after transformation (reply: 2)
1601. How many colonies to screen (colony PCR)? - (reply: 4)
1602. Primer Design - aligning sequences for probe/primer design (reply: 2)
1603. Cloning problem - (reply: 2)
1604. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
1605. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1606. In situ hybridization enigma - (reply: 1)
1607. A cloning nightmare - (reply: 8)
1608. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1609. RNA fragmentation - (reply: 3)
1610. RNA extraction - how many cells do I need at least?? (reply: 4)
1611. RNA extraction protocol for small amounts of cells - (reply: 2)
1612. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
1613. DNA sample contaminated with lipids - (reply: 8)
1614. cloning problem - (reply: 5)
1615. colony PCR inconsistent - (reply: 4)
1616. Newbie to Sequencing Question - (reply: 1)
1617. Problem with gel extraction of DNA. - (reply: 1)
1618. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
1619. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
1620. genomic DNA isolation - (reply: 1)
1592. 3īRACE problem - only smear - (reply: 4)
1593. salt for DNA precipitation - DNA purification (reply: 3)
1594. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1595. Not getting plasmid from miniprep - (reply: 7)
1596. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1597. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1598. Is this consider smearing? - (reply: 2)
1599. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1600. grow in broth but not on agar... - after transformation (reply: 2)
1601. How many colonies to screen (colony PCR)? - (reply: 4)
1602. Primer Design - aligning sequences for probe/primer design (reply: 2)
1603. Cloning problem - (reply: 2)
1604. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
1605. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1606. In situ hybridization enigma - (reply: 1)
1607. A cloning nightmare - (reply: 8)
1608. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1609. RNA fragmentation - (reply: 3)
1610. RNA extraction - how many cells do I need at least?? (reply: 4)
1611. RNA extraction protocol for small amounts of cells - (reply: 2)
1612. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
1613. DNA sample contaminated with lipids - (reply: 8)
1614. cloning problem - (reply: 5)
1615. colony PCR inconsistent - (reply: 4)
1616. Newbie to Sequencing Question - (reply: 1)
1617. Problem with gel extraction of DNA. - (reply: 1)
1618. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
1619. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
1620. genomic DNA isolation - (reply: 1)