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Top : New Forum Archives (2009-): : Molecular-Biology
1591. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1592. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1593. How long is "overnight"? - (reply: 3)
1594. Trizol stability - (reply: 1)
1595. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1596. Tube Openers - (reply: 3)
1597. taq man probes non specific? - (reply: 1)
1598. Help! low efficiency of transfection - (reply: 2)
1599. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1600. Cloning before sequencing- is it necessary??? - (reply: 13)
1601. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1602. Guanadine HCl for plasmid miniprep - (reply: 3)
1603. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1604. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1605. 3īRACE problem - only smear - (reply: 4)
1606. salt for DNA precipitation - DNA purification (reply: 3)
1607. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1608. Not getting plasmid from miniprep - (reply: 7)
1609. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1610. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1611. Is this consider smearing? - (reply: 2)
1612. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1613. grow in broth but not on agar... - after transformation (reply: 2)
1614. How many colonies to screen (colony PCR)? - (reply: 4)
1615. Primer Design - aligning sequences for probe/primer design (reply: 2)
1616. Cloning problem - (reply: 2)
1617. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
1618. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1619. In situ hybridization enigma - (reply: 1)
1620. A cloning nightmare - (reply: 8)