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Top : New Forum Archives (2009-): : Molecular-Biology
1591. Promoter "optimization" - (reply: 1)
1592. Adding Restriction Site to DNA - (reply: 3)
1593. how to do gel documentation - (reply: 2)
1594. DNA ext from paraffin-embedded tissue ... a dilemma ! - FFPE tissue refuse to lyse strange whitish clumps formed!! (reply: 1)
1595. Glycine linker - (reply: 2)
1596. Why does the amount of plamids transformed affect colony count? - (reply: 2)
1597. How to obatin intron sequence from cDNA sequence? - (reply: 1)
1598. genomic DNA extraction - why there appears a smear on the gel photo (reply: 11)
1599. Colony Pcr - primers - (reply: 5)
1600. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
1601. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
1602. Sequence size and insert size - Sequence size has larger base pair than insert size (reply: 2)
1603. QCMD samples - (reply: 5)
1604. competent cells - OD600 reading (reply: 6)
1605. Creating overhangs with PCR - (reply: 4)
1606. RNA: Is this degratation or something else? - (reply: 1)
1607. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1608. Is there any software that can be sued to design degenerate primers - (reply: 1)
1609. Primer Decontamination - (reply: 2)
1610. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1611. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1612. How long is "overnight"? - (reply: 3)
1613. Trizol stability - (reply: 1)
1614. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1615. Tube Openers - (reply: 3)
1616. taq man probes non specific? - (reply: 1)
1617. Help! low efficiency of transfection - (reply: 2)
1618. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1619. Cloning before sequencing- is it necessary??? - (reply: 13)
1620. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)