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1591. competent cells - OD600 reading (reply: 6)
1592. Creating overhangs with PCR - (reply: 4)
1593. RNA: Is this degratation or something else? - (reply: 1)
1594. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1595. Is there any software that can be sued to design degenerate primers - (reply: 1)
1596. Primer Decontamination - (reply: 2)
1597. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1598. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1599. How long is "overnight"? - (reply: 3)
1600. Trizol stability - (reply: 1)
1601. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1602. Tube Openers - (reply: 3)
1603. taq man probes non specific? - (reply: 1)
1604. Help! low efficiency of transfection - (reply: 2)
1605. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1606. Cloning before sequencing- is it necessary??? - (reply: 13)
1607. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1608. Guanadine HCl for plasmid miniprep - (reply: 3)
1609. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1610. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1611. 3īRACE problem - only smear - (reply: 4)
1612. salt for DNA precipitation - DNA purification (reply: 3)
1613. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1614. Not getting plasmid from miniprep - (reply: 7)
1615. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1616. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1617. Is this consider smearing? - (reply: 2)
1618. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1619. grow in broth but not on agar... - after transformation (reply: 2)
1620. How many colonies to screen (colony PCR)? - (reply: 4)
1592. Creating overhangs with PCR - (reply: 4)
1593. RNA: Is this degratation or something else? - (reply: 1)
1594. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1595. Is there any software that can be sued to design degenerate primers - (reply: 1)
1596. Primer Decontamination - (reply: 2)
1597. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1598. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1599. How long is "overnight"? - (reply: 3)
1600. Trizol stability - (reply: 1)
1601. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1602. Tube Openers - (reply: 3)
1603. taq man probes non specific? - (reply: 1)
1604. Help! low efficiency of transfection - (reply: 2)
1605. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1606. Cloning before sequencing- is it necessary??? - (reply: 13)
1607. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1608. Guanadine HCl for plasmid miniprep - (reply: 3)
1609. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1610. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1611. 3īRACE problem - only smear - (reply: 4)
1612. salt for DNA precipitation - DNA purification (reply: 3)
1613. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1614. Not getting plasmid from miniprep - (reply: 7)
1615. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1616. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1617. Is this consider smearing? - (reply: 2)
1618. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1619. grow in broth but not on agar... - after transformation (reply: 2)
1620. How many colonies to screen (colony PCR)? - (reply: 4)