Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1561. Why does the amount of plamids transformed affect colony count? - (reply: 2)
1562. How to obatin intron sequence from cDNA sequence? - (reply: 1)
1563. genomic DNA extraction - why there appears a smear on the gel photo (reply: 11)
1564. Colony Pcr - primers - (reply: 5)
1565. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
1566. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
1567. Sequence size and insert size - Sequence size has larger base pair than insert size (reply: 2)
1568. QCMD samples - (reply: 5)
1569. competent cells - OD600 reading (reply: 6)
1570. Creating overhangs with PCR - (reply: 4)
1571. RNA: Is this degratation or something else? - (reply: 1)
1572. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1573. Is there any software that can be sued to design degenerate primers - (reply: 1)
1574. Primer Decontamination - (reply: 2)
1575. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1576. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1577. How long is "overnight"? - (reply: 3)
1578. Trizol stability - (reply: 1)
1579. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1580. Tube Openers - (reply: 3)
1581. taq man probes non specific? - (reply: 1)
1582. Help! low efficiency of transfection - (reply: 2)
1583. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1584. Cloning before sequencing- is it necessary??? - (reply: 13)
1585. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1586. Guanadine HCl for plasmid miniprep - (reply: 3)
1587. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1588. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1589. 3īRACE problem - only smear - (reply: 4)
1590. salt for DNA precipitation - DNA purification (reply: 3)