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601. Low yield for small RNA isolation - (reply: 1)
602. Primers to amplify Kan operon from pet28 - (reply: 2)
603. Questions about proper technical approach - (reply: 6)
604. RNA extraction: low RCF by mistake - (reply: 1)
605. Avoid SDS in thermal lysis: whole blood? - (reply: 1)
606. What is the role of SV40 sequence in "5'UTR - GFP - SV40"? - (reply: 1)
607. Transfection of a non human gene into human cell line - (reply: 1)
608. DNA extraction from non-viable PBMCs - (reply: 2)
609. Visualizing RNA on blot - (reply: 1)
610. Can "3' - biotinylated oligo" be ligased with "5' phosphoryl - (reply: 3)
611. expression plasmids for tRNA - (reply: 6)
612. a promoter question, two promoter cassetts-related expression - (reply: 2)
613. Protein and RNA interaction - (reply: 1)
614. Transcription factors involved in mouse lung transfection - EMSA, pull-down- how - (reply: 3)
615. how can I remove the pigments during DNA extraction? - (reply: 1)
616. how to remove brown pigment in Sargassum DNA isolation? - (reply: 4)
617. difference between CDNA & CDS - (reply: 1)
618. improper recombination in transgenic mice - (reply: 1)
619. contamination of human cell lines with yeast - (reply: 3)
620. Barcoding PCR products - (reply: 18)
621. Re-culturing empty plasmid - (reply: 1)
622. IMAC nickel solution - (reply: 1)
623. Large scale Viral RNA purification Kit - (reply: 3)
624. UTR sequences after IRES - (reply: 1)
625. Storing Bacterial Culture - (reply: 1)
626. MTT: for 5 days - (reply: 1)
627. T7 promoter ending; to add or not to add GGG - (reply: 2)
628. Cytoplasmic mRNA isolation protocol - (reply: 1)
629. BSA in PCR reaction - (reply: 1)
630. Adding T7 promoter sequence to vector - (reply: 2)
602. Primers to amplify Kan operon from pet28 - (reply: 2)
603. Questions about proper technical approach - (reply: 6)
604. RNA extraction: low RCF by mistake - (reply: 1)
605. Avoid SDS in thermal lysis: whole blood? - (reply: 1)
606. What is the role of SV40 sequence in "5'UTR - GFP - SV40"? - (reply: 1)
607. Transfection of a non human gene into human cell line - (reply: 1)
608. DNA extraction from non-viable PBMCs - (reply: 2)
609. Visualizing RNA on blot - (reply: 1)
610. Can "3' - biotinylated oligo" be ligased with "5' phosphoryl - (reply: 3)
611. expression plasmids for tRNA - (reply: 6)
612. a promoter question, two promoter cassetts-related expression - (reply: 2)
613. Protein and RNA interaction - (reply: 1)
614. Transcription factors involved in mouse lung transfection - EMSA, pull-down- how - (reply: 3)
615. how can I remove the pigments during DNA extraction? - (reply: 1)
616. how to remove brown pigment in Sargassum DNA isolation? - (reply: 4)
617. difference between CDNA & CDS - (reply: 1)
618. improper recombination in transgenic mice - (reply: 1)
619. contamination of human cell lines with yeast - (reply: 3)
620. Barcoding PCR products - (reply: 18)
621. Re-culturing empty plasmid - (reply: 1)
622. IMAC nickel solution - (reply: 1)
623. Large scale Viral RNA purification Kit - (reply: 3)
624. UTR sequences after IRES - (reply: 1)
625. Storing Bacterial Culture - (reply: 1)
626. MTT: for 5 days - (reply: 1)
627. T7 promoter ending; to add or not to add GGG - (reply: 2)
628. Cytoplasmic mRNA isolation protocol - (reply: 1)
629. BSA in PCR reaction - (reply: 1)
630. Adding T7 promoter sequence to vector - (reply: 2)