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Top : New Forum Archives (2009-): : Molecular-Biology
601. Avoid SDS in thermal lysis: whole blood? - (reply: 1)
602. What is the role of SV40 sequence in "5'UTR - GFP - SV40"? - (reply: 1)
603. Transfection of a non human gene into human cell line - (reply: 1)
604. DNA extraction from non-viable PBMCs - (reply: 2)
605. Visualizing RNA on blot - (reply: 1)
606. Can "3' - biotinylated oligo" be ligased with "5' phosphoryl - (reply: 3)
607. expression plasmids for tRNA - (reply: 6)
608. a promoter question, two promoter cassetts-related expression - (reply: 2)
609. Protein and RNA interaction - (reply: 1)
610. Transcription factors involved in mouse lung transfection - EMSA, pull-down- how - (reply: 3)
611. how can I remove the pigments during DNA extraction? - (reply: 1)
612. how to remove brown pigment in Sargassum DNA isolation? - (reply: 4)
613. difference between CDNA & CDS - (reply: 1)
614. improper recombination in transgenic mice - (reply: 1)
615. contamination of human cell lines with yeast - (reply: 3)
616. Barcoding PCR products - (reply: 18)
617. Re-culturing empty plasmid - (reply: 1)
618. IMAC nickel solution - (reply: 1)
619. Large scale Viral RNA purification Kit - (reply: 3)
620. UTR sequences after IRES - (reply: 1)
621. Storing Bacterial Culture - (reply: 1)
622. MTT: for 5 days - (reply: 1)
623. T7 promoter ending; to add or not to add GGG - (reply: 2)
624. Cytoplasmic mRNA isolation protocol - (reply: 1)
625. BSA in PCR reaction - (reply: 1)
626. Adding T7 promoter sequence to vector - (reply: 2)
627. Can CMV promoter start transcription in prokaryote cells - (reply: 1)
628. Taq vs Phusion polymerases - specificity - (reply: 8)
629. RNA isolation of murine pancreatic islets - poor ratios - (reply: 3)
630. Tagging a gene with HA and FLAG using PCR - (reply: 1)