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601. How base pairs upstream of gene to include for the promoter? - (reply: 3)
602. Neomycin resistance sequence - (reply: 1)
603. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
604. polyacrylamide RNA gel - (reply: 4)
605. Making a standard curve from bacterial suspensions - (reply: 1)
606. Is my RNA degraded? - (reply: 7)
607. Phosphotyrosine western blotting, HELP! - (reply: 1)
608. Bacteriophage genome extraction - (reply: 1)
609. Fast, cheap and dependable DNA extraction protocol for students? - (reply: 3)
610. western blot troubleshooting - (reply: 5)
611. Sanger Sequencing RNAse Interference? - (reply: 1)
612. isolating DNA from cells in suspension - (reply: 5)
613. survival strategy of bacteria - (reply: 4)
614. can restriction enzymes be stored at -70°C? - (reply: 3)
615. Sequencing Primers and Plasmids - (reply: 3)
616. Dam Dcm Negative Strain - (reply: 2)
617. Restriction Enzymes - (reply: 4)
618. RNA isolation - (reply: 1)
619. Qiagen re using column - (reply: 5)
620. Low yield for small RNA isolation - (reply: 1)
621. Primers to amplify Kan operon from pet28 - (reply: 2)
622. Questions about proper technical approach - (reply: 6)
623. RNA extraction: low RCF by mistake - (reply: 1)
624. Avoid SDS in thermal lysis: whole blood? - (reply: 1)
625. What is the role of SV40 sequence in "5'UTR - GFP - SV40"? - (reply: 1)
626. Transfection of a non human gene into human cell line - (reply: 1)
627. DNA extraction from non-viable PBMCs - (reply: 2)
628. Visualizing RNA on blot - (reply: 1)
629. Can "3' - biotinylated oligo" be ligased with "5' phosphoryl - (reply: 3)
630. expression plasmids for tRNA - (reply: 6)
602. Neomycin resistance sequence - (reply: 1)
603. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
604. polyacrylamide RNA gel - (reply: 4)
605. Making a standard curve from bacterial suspensions - (reply: 1)
606. Is my RNA degraded? - (reply: 7)
607. Phosphotyrosine western blotting, HELP! - (reply: 1)
608. Bacteriophage genome extraction - (reply: 1)
609. Fast, cheap and dependable DNA extraction protocol for students? - (reply: 3)
610. western blot troubleshooting - (reply: 5)
611. Sanger Sequencing RNAse Interference? - (reply: 1)
612. isolating DNA from cells in suspension - (reply: 5)
613. survival strategy of bacteria - (reply: 4)
614. can restriction enzymes be stored at -70°C? - (reply: 3)
615. Sequencing Primers and Plasmids - (reply: 3)
616. Dam Dcm Negative Strain - (reply: 2)
617. Restriction Enzymes - (reply: 4)
618. RNA isolation - (reply: 1)
619. Qiagen re using column - (reply: 5)
620. Low yield for small RNA isolation - (reply: 1)
621. Primers to amplify Kan operon from pet28 - (reply: 2)
622. Questions about proper technical approach - (reply: 6)
623. RNA extraction: low RCF by mistake - (reply: 1)
624. Avoid SDS in thermal lysis: whole blood? - (reply: 1)
625. What is the role of SV40 sequence in "5'UTR - GFP - SV40"? - (reply: 1)
626. Transfection of a non human gene into human cell line - (reply: 1)
627. DNA extraction from non-viable PBMCs - (reply: 2)
628. Visualizing RNA on blot - (reply: 1)
629. Can "3' - biotinylated oligo" be ligased with "5' phosphoryl - (reply: 3)
630. expression plasmids for tRNA - (reply: 6)