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601. Best plasmid for GFP + Mammalian Selection - (reply: 1)
602. Transformation /Cloning Problem - (reply: 3)
603. Ligation Problem - (reply: 4)
604. a robust multi-gene cloning method - (reply: 2)
605. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
606. Colony lift hybridisation positive but clones are negative for insert DNA - (reply: 1)
607. Mortar and Pestle for RNA Extraction - (reply: 1)
608. Any free software for drawing genes? - (reply: 9)
609. TREx pcDNA6/TR - (reply: 2)
610. Plasmid, gene orientation, insert orientation. - (reply: 1)
611. Need stable GFP expression in IPS and embryonic SCs - (reply: 1)
612. good tutorials/protocols/books on gene cloning - (reply: 2)
613. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
614. yeast growth in SC-ura medium - (reply: 1)
615. Optimizing Trizol mRNA extraction - (reply: 3)
616. Expression in trans - (reply: 1)
617. DNA Storage - (reply: 10)
618. RBC LYSIS BUFFER - a good substitute of sucrose? - (reply: 5)
619. Luciferase assay - expected range of raw reading values - (reply: 1)
620. compare between 2 or more genes - (reply: 1)
621. sample preservation for RNA extraction - (reply: 1)
622. Designing primers for the ORFs - (reply: 9)
623. Bioinformatics Software - (reply: 2)
624. Low yields with quiagen midi kit - (reply: 2)
625. RNA extraction from blood stain - (reply: 1)
626. Knocking out non-essential gene - (reply: 2)
627. PCR with phophorylated primers - (reply: 1)
628. Nanodrop absorbtion at 290 nm??? - (reply: 1)
629. Plasmid sequencing without primers - (reply: 1)
630. Either too low or too high a260/a230 - (reply: 5)
602. Transformation /Cloning Problem - (reply: 3)
603. Ligation Problem - (reply: 4)
604. a robust multi-gene cloning method - (reply: 2)
605. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
606. Colony lift hybridisation positive but clones are negative for insert DNA - (reply: 1)
607. Mortar and Pestle for RNA Extraction - (reply: 1)
608. Any free software for drawing genes? - (reply: 9)
609. TREx pcDNA6/TR - (reply: 2)
610. Plasmid, gene orientation, insert orientation. - (reply: 1)
611. Need stable GFP expression in IPS and embryonic SCs - (reply: 1)
612. good tutorials/protocols/books on gene cloning - (reply: 2)
613. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
614. yeast growth in SC-ura medium - (reply: 1)
615. Optimizing Trizol mRNA extraction - (reply: 3)
616. Expression in trans - (reply: 1)
617. DNA Storage - (reply: 10)
618. RBC LYSIS BUFFER - a good substitute of sucrose? - (reply: 5)
619. Luciferase assay - expected range of raw reading values - (reply: 1)
620. compare between 2 or more genes - (reply: 1)
621. sample preservation for RNA extraction - (reply: 1)
622. Designing primers for the ORFs - (reply: 9)
623. Bioinformatics Software - (reply: 2)
624. Low yields with quiagen midi kit - (reply: 2)
625. RNA extraction from blood stain - (reply: 1)
626. Knocking out non-essential gene - (reply: 2)
627. PCR with phophorylated primers - (reply: 1)
628. Nanodrop absorbtion at 290 nm??? - (reply: 1)
629. Plasmid sequencing without primers - (reply: 1)
630. Either too low or too high a260/a230 - (reply: 5)