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Top : New Forum Archives (2009-): : Molecular-Biology
391. 5'-azacytidine treatment - (reply: 1)
392. leaky promoter - (reply: 1)
393. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
394. SAP dephosphorylation - (reply: 1)
395. Determining in frame in modified vector - (reply: 1)
396. Deleting restriction enzyme sites - (reply: 1)
397. Creating primers to add restriction sites to vector backbone - (reply: 7)
398. cDNA test on 1% agarose gel - (reply: 3)
399. Plasmid isolation from gram positive bacteria - (reply: 2)
400. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
401. rsaI enzyme - (reply: 1)
402. How to check for the presence of plasmid? - (reply: 3)
403. Extracting total RNA from woody plant roots - (reply: 2)
404. Quantify total DNA in small cell lysate...? - (reply: 1)
405. Quantify DNA in gel electrophoresis bands - (reply: 7)
406. Protein extraction and isolectric focusing - (reply: 1)
407. getting strange data in Luciferase-renilla assay - (reply: 2)
408. Homozygote DNA but Heterozygote RNA? - (reply: 2)
409. sacB Counter-Selection to generate double cross-over deletion mutants - (reply: 8)
410. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
411. What is the necessary time for lipofectamin to make complex with pre-miRNA oligo - (reply: 1)
412. what is the ORFeome clone? - (reply: 2)
413. ligation with one ristrection site/enzyme - (reply: 3)
414. No amplification during RT-PCR - (reply: 4)
415. rescue cloning of a transposon mutant - (reply: 2)
416. Dephosphorylation - (reply: 7)
417. study of receptors in cell lines - (reply: 1)
418. Mouse genotyping issues - (reply: 1)
419. In-situ hybridization - (reply: 1)
420. His tag introduction in to gene and primer design - (reply: 2)