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2101. Do you lose RNA by quick freezing? - (reply: 5)
2102. obatining full nucelotide sequences for genes - where can i find them (reply: 4)
2103. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
2104. Converting RE sites - (reply: 2)
2105. Can DNA be solubilized in hydrochloric acid? - (reply: 2)
2106. house keeping gene in transgenic mice. - (reply: 1)
2107. RNA cleanup methods (WITHOUT USING KITS!!) - protocol for removing phenol contamination (reply: 5)
2108. Detect gene in stable cell line, - (reply: 4)
2109. in vivo expression technology - IVET (reply: 2)
2110. basic math question - Molarity (reply: 2)
2111. EMSA supershift - increased band intensity? - (reply: 1)
2112. Who can explain this? Weird cloning problem.... - (reply: 6)
2113. RNA extraction - Which is which? (reply: 6)
2114. DNase I digestion - how long the fragments are at the end??? (reply: 3)
2115. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
2116. pIRESneo for mammalian cells transfection - (reply: 4)
2117. H9C2 - cell line - Need clarification (reply: 1)
2118. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
2119. no band in gel - gel electrophoresis (reply: 1)
2120. stratagene site directed mutagenesis - deletion of single base nucleotide (reply: 3)
2121. How to increase A260/280 and A260/230 ratio for microarray experiment? - (reply: 8)
2122. western blot stripping buffer - please recomend a stripping buffer for re-bloting (reply: 2)
2123. T7 and T7lac - (reply: 1)
2124. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
2125. Primer stock confusion - (reply: 6)
2126. Pancreas RNA extraction - improve protocol needed (reply: 2)
2127. Genomic DNA contamination in RNA sample - (reply: 2)
2128. Isolation DNA and protein from a single sample - (reply: 5)
2129. lipofectamine - (reply: 3)
2130. Gel Analysis Software - which to use (reply: 3)
2102. obatining full nucelotide sequences for genes - where can i find them (reply: 4)
2103. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
2104. Converting RE sites - (reply: 2)
2105. Can DNA be solubilized in hydrochloric acid? - (reply: 2)
2106. house keeping gene in transgenic mice. - (reply: 1)
2107. RNA cleanup methods (WITHOUT USING KITS!!) - protocol for removing phenol contamination (reply: 5)
2108. Detect gene in stable cell line, - (reply: 4)
2109. in vivo expression technology - IVET (reply: 2)
2110. basic math question - Molarity (reply: 2)
2111. EMSA supershift - increased band intensity? - (reply: 1)
2112. Who can explain this? Weird cloning problem.... - (reply: 6)
2113. RNA extraction - Which is which? (reply: 6)
2114. DNase I digestion - how long the fragments are at the end??? (reply: 3)
2115. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
2116. pIRESneo for mammalian cells transfection - (reply: 4)
2117. H9C2 - cell line - Need clarification (reply: 1)
2118. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
2119. no band in gel - gel electrophoresis (reply: 1)
2120. stratagene site directed mutagenesis - deletion of single base nucleotide (reply: 3)
2121. How to increase A260/280 and A260/230 ratio for microarray experiment? - (reply: 8)
2122. western blot stripping buffer - please recomend a stripping buffer for re-bloting (reply: 2)
2123. T7 and T7lac - (reply: 1)
2124. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
2125. Primer stock confusion - (reply: 6)
2126. Pancreas RNA extraction - improve protocol needed (reply: 2)
2127. Genomic DNA contamination in RNA sample - (reply: 2)
2128. Isolation DNA and protein from a single sample - (reply: 5)
2129. lipofectamine - (reply: 3)
2130. Gel Analysis Software - which to use (reply: 3)