Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1201. Radioactive calculation - (reply: 3)
1202. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1203. invitro mutagenesis - (reply: 5)
1204. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1205. Extra band on gel after miniprep - (reply: 6)
1206. 3 distinct bands of gDNA on gel - (reply: 2)
1207. PCR Mastermix - (reply: 4)
1208. Unsuccessful self-ligation - (reply: 4)
1209. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1210. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1211. DNA extraction from E. coli - solutions needed? (reply: 6)
1212. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1213. Frequency of restriction enzyme sites in a genome - (reply: 5)
1214. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1215. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1216. Internal Control RNA or DNA - (reply: 4)
1217. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1218. RNA extraction - (reply: 3)
1219. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1220. finding exons for a gene, having the coding sequence only - (reply: 4)
1221. colonies without insert!!! - molecular biology (reply: 1)
1222. Effect of phosphoric acid on a kinase assay - (reply: 2)
1223. Proteinase K stock solution - (reply: 3)
1224. Total RNA only produce 1 band in gel - (reply: 2)
1225. Advice on amount of starting template for subcloning - (reply: 3)
1226. New to Gene cloning - Plasmid vector preparation (reply: 2)
1227. PCR for MULTIPLE mutagenesis - (reply: 4)
1228. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
1229. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
1230. Induction strength : IPTG vs lactose - which one is stronger? (reply: 2)