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Top : New Forum Archives (2009-): : Molecular-Biology
1201. Genomic DNA purity...??? - strange 260/280 and 260/230 ratios (reply: 7)
1202. Problems with pJET1,2Blunt - (reply: 1)
1203. DH5 Alpha Lambda pir and Conjugation - (reply: 1)
1204. Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
1205. Overexpression plasmid is not expressing protein - (reply: 13)
1206. Why shouldn't you clone using expression bacteria - (reply: 6)
1207. Unstable A260/A230 in RNA isolation - (reply: 2)
1208. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
1209. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
1210. Radioactive calculation - (reply: 3)
1211. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1212. invitro mutagenesis - (reply: 5)
1213. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1214. Extra band on gel after miniprep - (reply: 6)
1215. 3 distinct bands of gDNA on gel - (reply: 2)
1216. PCR Mastermix - (reply: 4)
1217. Unsuccessful self-ligation - (reply: 4)
1218. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1219. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1220. DNA extraction from E. coli - solutions needed? (reply: 6)
1221. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1222. Frequency of restriction enzyme sites in a genome - (reply: 5)
1223. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1224. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1225. Internal Control RNA or DNA - (reply: 4)
1226. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1227. RNA extraction - (reply: 3)
1228. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1229. finding exons for a gene, having the coding sequence only - (reply: 4)
1230. colonies without insert!!! - molecular biology (reply: 1)