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Top : New Forum Archives (2009-): : Molecular-Biology
1201. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
1202. Possible solvents for RNA - (reply: 2)
1203. weird bands after minipreps - molecular cloning (reply: 2)
1204. Large fragment amplification failed - (reply: 3)
1205. purification of PCR product for cloning in a vector - (reply: 3)
1206. elimination of Protein interference from DNA - for RT-PCR (reply: 2)
1207. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
1208. Adapter ligation problem - (reply: 5)
1209. Primer design with a tag - (reply: 4)
1210. Lithium electrophoresis buffer - Why does it not work? (reply: 2)
1211. Weird migration of double digested plasmid - (reply: 4)
1212. Run agarose/formaldehyde gel for 5 mins before loading samples? Why? - (reply: 1)
1213. Myc tags - (reply: 1)
1214. MOPS: How do you sterlise yours? - (reply: 2)
1215. Which gel concentration - (reply: 4)
1216. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
1217. PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
1218. MINI Dialysis Units - below 2K MWCO? (reply: 1)
1219. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
1220. Rotten Fish Smell in TAE Buffer - Stinky TAE (reply: 3)
1221. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
1222. Combining forward and reverse primer gives different size on gel - (reply: 2)
1223. Help for co-transfection of proteins and plasmid - (reply: 1)
1224. PCR of complement genomic DNA - (reply: 3)
1225. RNAi is somatic and germline cells - (reply: 3)
1226. subcloning into bacterial expression vector - (reply: 1)
1227. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
1228. cloning into F1 ori of expression vector??? - (reply: 1)
1229. primers for fungal identification - (reply: 9)
1230. protein overexpression in HeLa cells - (reply: 1)