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1201. Cloning and expressing a mystery 71KDa protein - (reply: 9)
1202. why 12 h culture for plasmid prep? - (reply: 2)
1203. Insert magically shortening??? - Cloning issues (reply: 2)
1204. Transformation issue - (reply: 1)
1205. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
1206. PCR yield in ng/ul - (reply: 1)
1207. How to add a T7 terminator to a vector? - (reply: 4)
1208. Dna after gel extraction, single or double stranded? - (reply: 1)
1209. Checking RNA on gel after DIG labeling - (reply: 1)
1210. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
1211. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
1212. DNA-PK inhibitor - Which chemical is the best? (reply: 1)
1213. some mathematics help - meaning of 5.0E+03 (reply: 4)
1214. Storage conditions - (reply: 4)
1215. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
1216. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
1217. Stability of DNA at RT - (reply: 1)
1218. yeast expression (gal1-promotor) - expression in glucose?? (reply: 1)
1219. TRIZol Reagent additional reagents & protocol clarifications - (reply: 4)
1220. Is it possible to islate strong negative charged protein - (reply: 1)
1221. DIG southern blot - (reply: 2)
1222. Real time PCR in the presence of heme - (reply: 3)
1223. Protein expression vectors Please help - (reply: 2)
1224. removal of residual ethanol - wht to do if i have ETOH in my eluate? (reply: 2)
1225. Loss of RNA conc. and quality in Qiagen RNeasy Protocol - (reply: 4)
1226. issues in PCR amplification - (reply: 2)
1227. extraa bases before RE site - Reply urgent!! (reply: 1)
1228. vectors please help - vectors cleavage sites (reply: 1)
1229. siRNA transfection advise - Need some help on siRNA knockdowns (reply: 1)
1230. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
1202. why 12 h culture for plasmid prep? - (reply: 2)
1203. Insert magically shortening??? - Cloning issues (reply: 2)
1204. Transformation issue - (reply: 1)
1205. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
1206. PCR yield in ng/ul - (reply: 1)
1207. How to add a T7 terminator to a vector? - (reply: 4)
1208. Dna after gel extraction, single or double stranded? - (reply: 1)
1209. Checking RNA on gel after DIG labeling - (reply: 1)
1210. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
1211. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
1212. DNA-PK inhibitor - Which chemical is the best? (reply: 1)
1213. some mathematics help - meaning of 5.0E+03 (reply: 4)
1214. Storage conditions - (reply: 4)
1215. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
1216. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
1217. Stability of DNA at RT - (reply: 1)
1218. yeast expression (gal1-promotor) - expression in glucose?? (reply: 1)
1219. TRIZol Reagent additional reagents & protocol clarifications - (reply: 4)
1220. Is it possible to islate strong negative charged protein - (reply: 1)
1221. DIG southern blot - (reply: 2)
1222. Real time PCR in the presence of heme - (reply: 3)
1223. Protein expression vectors Please help - (reply: 2)
1224. removal of residual ethanol - wht to do if i have ETOH in my eluate? (reply: 2)
1225. Loss of RNA conc. and quality in Qiagen RNeasy Protocol - (reply: 4)
1226. issues in PCR amplification - (reply: 2)
1227. extraa bases before RE site - Reply urgent!! (reply: 1)
1228. vectors please help - vectors cleavage sites (reply: 1)
1229. siRNA transfection advise - Need some help on siRNA knockdowns (reply: 1)
1230. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)