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1201. Double digestion with NcoI and XhoI - (reply: 3)
1202. U6 promoter - (reply: 4)
1203. expression of promoter T7 - (reply: 6)
1204. SLIM mutagenesis of large plasmids - (reply: 8)
1205. The Best Gel Extraction Kits - The Best Gel Extraction Kits (reply: 9)
1206. Can you receommend a vector? - (reply: 1)
1207. DNA purity - (reply: 1)
1208. Mutagenesis, deletion - what is the best kit/protocol (reply: 6)
1209. Ethidium Bromide Stability - (reply: 1)
1210. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
1211. primer design - microalgae (reply: 2)
1212. Trouble to UNDERSTAND gene replacement results - (reply: 1)
1213. Would clean dry hot air kill bacteria? - (reply: 6)
1214. Genomic DNA contamination in my RNA sample? - (reply: 8)
1215. Cloning and expressing a mystery 71KDa protein - (reply: 9)
1216. why 12 h culture for plasmid prep? - (reply: 2)
1217. Insert magically shortening??? - Cloning issues (reply: 2)
1218. Transformation issue - (reply: 1)
1219. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
1220. PCR yield in ng/ul - (reply: 1)
1221. How to add a T7 terminator to a vector? - (reply: 4)
1222. Dna after gel extraction, single or double stranded? - (reply: 1)
1223. Checking RNA on gel after DIG labeling - (reply: 1)
1224. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
1225. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
1226. DNA-PK inhibitor - Which chemical is the best? (reply: 1)
1227. some mathematics help - meaning of 5.0E+03 (reply: 4)
1228. Storage conditions - (reply: 4)
1229. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
1230. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
1202. U6 promoter - (reply: 4)
1203. expression of promoter T7 - (reply: 6)
1204. SLIM mutagenesis of large plasmids - (reply: 8)
1205. The Best Gel Extraction Kits - The Best Gel Extraction Kits (reply: 9)
1206. Can you receommend a vector? - (reply: 1)
1207. DNA purity - (reply: 1)
1208. Mutagenesis, deletion - what is the best kit/protocol (reply: 6)
1209. Ethidium Bromide Stability - (reply: 1)
1210. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
1211. primer design - microalgae (reply: 2)
1212. Trouble to UNDERSTAND gene replacement results - (reply: 1)
1213. Would clean dry hot air kill bacteria? - (reply: 6)
1214. Genomic DNA contamination in my RNA sample? - (reply: 8)
1215. Cloning and expressing a mystery 71KDa protein - (reply: 9)
1216. why 12 h culture for plasmid prep? - (reply: 2)
1217. Insert magically shortening??? - Cloning issues (reply: 2)
1218. Transformation issue - (reply: 1)
1219. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
1220. PCR yield in ng/ul - (reply: 1)
1221. How to add a T7 terminator to a vector? - (reply: 4)
1222. Dna after gel extraction, single or double stranded? - (reply: 1)
1223. Checking RNA on gel after DIG labeling - (reply: 1)
1224. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
1225. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
1226. DNA-PK inhibitor - Which chemical is the best? (reply: 1)
1227. some mathematics help - meaning of 5.0E+03 (reply: 4)
1228. Storage conditions - (reply: 4)
1229. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
1230. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)