Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1171. Problems with pJET1,2Blunt - (reply: 1)
1172. DH5 Alpha Lambda pir and Conjugation - (reply: 1)
1173. Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
1174. Overexpression plasmid is not expressing protein - (reply: 13)
1175. Why shouldn't you clone using expression bacteria - (reply: 6)
1176. Unstable A260/A230 in RNA isolation - (reply: 2)
1177. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
1178. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
1179. Radioactive calculation - (reply: 3)
1180. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1181. invitro mutagenesis - (reply: 5)
1182. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1183. Extra band on gel after miniprep - (reply: 6)
1184. 3 distinct bands of gDNA on gel - (reply: 2)
1185. PCR Mastermix - (reply: 4)
1186. Unsuccessful self-ligation - (reply: 4)
1187. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1188. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1189. DNA extraction from E. coli - solutions needed? (reply: 6)
1190. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1191. Frequency of restriction enzyme sites in a genome - (reply: 5)
1192. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1193. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1194. Internal Control RNA or DNA - (reply: 4)
1195. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1196. RNA extraction - (reply: 3)
1197. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1198. finding exons for a gene, having the coding sequence only - (reply: 4)
1199. colonies without insert!!! - molecular biology (reply: 1)
1200. Effect of phosphoric acid on a kinase assay - (reply: 2)