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1171. RNA extraction protocol for small amounts of cells - (reply: 2)
1172. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
1173. DNA sample contaminated with lipids - (reply: 8)
1174. cloning problem - (reply: 5)
1175. colony PCR inconsistent - (reply: 4)
1176. Newbie to Sequencing Question - (reply: 1)
1177. Problem with gel extraction of DNA. - (reply: 1)
1178. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
1179. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
1180. genomic DNA isolation - (reply: 1)
1181. E. coli not growing in liquid cultures :( - (reply: 4)
1182. Tricine SDS-PAGE for plant mitochondria - Membrane proteomics (reply: 1)
1183. DNA product almost 1500 bp - (reply: 1)
1184. Slightly too high 260:280 and 260:230 ratios from gDNA extraction - Should I care? (reply: 2)
1185. Blunt end filling - Blunt end filling (reply: 1)
1186. GHOST in Electrophoresis - (reply: 18)
1187. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
1188. Transformation background problem - high background in negative control (reply: 9)
1189. Akrylamide Gels - (reply: 2)
1190. Does unautoclaved DEPC inhibit northern blotting? - (reply: 2)
1191. Miniprep and sequencing - (reply: 4)
1192. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
1193. Protein TAG for affinity purification - (reply: 1)
1194. Blunt-Cohesive ligation - Should I change the conditions? (reply: 2)
1195. GFP fused proteins - i'm going to cry... (reply: 3)
1196. what's the meaning? - HEAP (reply: 2)
1197. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
1198. Making gels in advance - (reply: 2)
1199. Phenol-chloroform problem - (reply: 1)
1200. Cloning <100 bp fragments into a plasmid - (reply: 7)
1172. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
1173. DNA sample contaminated with lipids - (reply: 8)
1174. cloning problem - (reply: 5)
1175. colony PCR inconsistent - (reply: 4)
1176. Newbie to Sequencing Question - (reply: 1)
1177. Problem with gel extraction of DNA. - (reply: 1)
1178. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
1179. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
1180. genomic DNA isolation - (reply: 1)
1181. E. coli not growing in liquid cultures :( - (reply: 4)
1182. Tricine SDS-PAGE for plant mitochondria - Membrane proteomics (reply: 1)
1183. DNA product almost 1500 bp - (reply: 1)
1184. Slightly too high 260:280 and 260:230 ratios from gDNA extraction - Should I care? (reply: 2)
1185. Blunt end filling - Blunt end filling (reply: 1)
1186. GHOST in Electrophoresis - (reply: 18)
1187. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
1188. Transformation background problem - high background in negative control (reply: 9)
1189. Akrylamide Gels - (reply: 2)
1190. Does unautoclaved DEPC inhibit northern blotting? - (reply: 2)
1191. Miniprep and sequencing - (reply: 4)
1192. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
1193. Protein TAG for affinity purification - (reply: 1)
1194. Blunt-Cohesive ligation - Should I change the conditions? (reply: 2)
1195. GFP fused proteins - i'm going to cry... (reply: 3)
1196. what's the meaning? - HEAP (reply: 2)
1197. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
1198. Making gels in advance - (reply: 2)
1199. Phenol-chloroform problem - (reply: 1)
1200. Cloning <100 bp fragments into a plasmid - (reply: 7)