Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1171. Mutagenesis, deletion - what is the best kit/protocol (reply: 6)
1172. Ethidium Bromide Stability - (reply: 1)
1173. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
1174. primer design - microalgae (reply: 2)
1175. Trouble to UNDERSTAND gene replacement results - (reply: 1)
1176. Would clean dry hot air kill bacteria? - (reply: 6)
1177. Genomic DNA contamination in my RNA sample? - (reply: 8)
1178. Cloning and expressing a mystery 71KDa protein - (reply: 9)
1179. why 12 h culture for plasmid prep? - (reply: 2)
1180. Insert magically shortening??? - Cloning issues (reply: 2)
1181. Transformation issue - (reply: 1)
1182. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
1183. PCR yield in ng/ul - (reply: 1)
1184. How to add a T7 terminator to a vector? - (reply: 4)
1185. Dna after gel extraction, single or double stranded? - (reply: 1)
1186. Checking RNA on gel after DIG labeling - (reply: 1)
1187. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
1188. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
1189. DNA-PK inhibitor - Which chemical is the best? (reply: 1)
1190. some mathematics help - meaning of 5.0E+03 (reply: 4)
1191. Storage conditions - (reply: 4)
1192. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
1193. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
1194. Stability of DNA at RT - (reply: 1)
1195. yeast expression (gal1-promotor) - expression in glucose?? (reply: 1)
1196. TRIZol Reagent additional reagents & protocol clarifications - (reply: 4)
1197. Is it possible to islate strong negative charged protein - (reply: 1)
1198. DIG southern blot - (reply: 2)
1199. Real time PCR in the presence of heme - (reply: 3)
1200. Protein expression vectors Please help - (reply: 2)