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Top : New Forum Archives (2009-): : Molecular-Biology
1171. Double digestion with NcoI and XhoI - (reply: 3)
1172. U6 promoter - (reply: 4)
1173. expression of promoter T7 - (reply: 6)
1174. SLIM mutagenesis of large plasmids - (reply: 8)
1175. The Best Gel Extraction Kits - The Best Gel Extraction Kits (reply: 9)
1176. Can you receommend a vector? - (reply: 1)
1177. DNA purity - (reply: 1)
1178. Mutagenesis, deletion - what is the best kit/protocol (reply: 6)
1179. Ethidium Bromide Stability - (reply: 1)
1180. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
1181. primer design - microalgae (reply: 2)
1182. Trouble to UNDERSTAND gene replacement results - (reply: 1)
1183. Would clean dry hot air kill bacteria? - (reply: 6)
1184. Genomic DNA contamination in my RNA sample? - (reply: 8)
1185. Cloning and expressing a mystery 71KDa protein - (reply: 9)
1186. why 12 h culture for plasmid prep? - (reply: 2)
1187. Insert magically shortening??? - Cloning issues (reply: 2)
1188. Transformation issue - (reply: 1)
1189. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
1190. PCR yield in ng/ul - (reply: 1)
1191. How to add a T7 terminator to a vector? - (reply: 4)
1192. Dna after gel extraction, single or double stranded? - (reply: 1)
1193. Checking RNA on gel after DIG labeling - (reply: 1)
1194. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
1195. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
1196. DNA-PK inhibitor - Which chemical is the best? (reply: 1)
1197. some mathematics help - meaning of 5.0E+03 (reply: 4)
1198. Storage conditions - (reply: 4)
1199. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
1200. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)