Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
2461. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
2462. problems with DNase1 footprinting - DNA won't enter a denaturing urea polyacrylamide gel (reply: 2)
2463. TOP10F' - Can they be used for regular vector propagation? (reply: 1)
2464. Gel shift with large DNA fragments (>1 kb) - Can I still do EMSA? (reply: 1)
2465. re-amplification in real-time PCR - (reply: 1)
2466. 3'RACE and the polyA tail - why only one band? (reply: 2)
2467. midi-prep - (reply: 5)
2468. Degenerative primers = multiple products? - (reply: 5)
2469. 96 Well minipreps? - (reply: 1)
2470. DNAse activity in room temperature - (reply: 3)
2471. Reverse transcribing organically contaminated RNA - (reply: 2)
2472. Phylogenetic interpretation - (reply: 1)
2473. Do you lose RNA by quick freezing? - (reply: 5)
2474. obatining full nucelotide sequences for genes - where can i find them (reply: 4)
2475. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
2476. Converting RE sites - (reply: 2)
2477. Can DNA be solubilized in hydrochloric acid? - (reply: 2)
2478. house keeping gene in transgenic mice. - (reply: 1)
2479. RNA cleanup methods (WITHOUT USING KITS!!) - protocol for removing phenol contamination (reply: 5)
2480. Detect gene in stable cell line, - (reply: 4)
2481. in vivo expression technology - IVET (reply: 2)
2482. basic math question - Molarity (reply: 2)
2483. EMSA supershift - increased band intensity? - (reply: 1)
2484. Who can explain this? Weird cloning problem.... - (reply: 6)
2485. RNA extraction - Which is which? (reply: 6)
2486. DNase I digestion - how long the fragments are at the end??? (reply: 3)
2487. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
2488. pIRESneo for mammalian cells transfection - (reply: 4)
2489. H9C2 - cell line - Need clarification (reply: 1)
2490. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)