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271. Top10 and DH10B need IPTG for induction? - (reply: 1)
272. Cell lysis and DNA quantification - (reply: 1)
273. New (free) plasmid mapping program - (reply: 4)
274. E coli culture cannot grow overnight. - (reply: 11)
275. Problem with in vitro transcription - (reply: 1)
276. excess amount of primers - (reply: 2)
277. Dehydrating and Reconstituting primers - (reply: 15)
278. Trouble with PCR of short sequence - (reply: 3)
279. EtBr decontamination of gel doc system - (reply: 2)
280. RNA Precipitation - (reply: 1)
281. filling the digested vector and insert for blunt end cloning - (reply: 2)
282. Identifying CpG islands on a promoter... - (reply: 1)
283. Bacterial DNA extraction/purification from animal tissue - (reply: 1)
284. Help with plasmid extraction and redissolving? - (reply: 5)
285. Thick 100-bp band disappeared under UV - (reply: 3)
286. codon optimization for CHO cells - (reply: 2)
287. Mutation in 3'UTR - (reply: 2)
288. Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
289. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
290. Can you store DNA in loading dye buffer short term? - (reply: 2)
291. Heavy and light Chain - (reply: 9)
292. Troubles with PCR (Rosa26 locus in mouse line) VERY CONFUSING! - (reply: 6)
293. Quick method for DNA extraction using lysis - (reply: 4)
294. Editing out secondary potential ORFs? - (reply: 2)
295. Sterility issues with plasmid reconstitution buffer? - (reply: 3)
296. Heating elution buffer for plasmid prep - (reply: 1)
297. qPCR and miRNA isolation - (reply: 4)
298. Yeast Two Hybrid system - (reply: 7)
299. how much purify protein nedded for IP - (reply: 9)
300. lyophilized protein dilution - (reply: 6)
272. Cell lysis and DNA quantification - (reply: 1)
273. New (free) plasmid mapping program - (reply: 4)
274. E coli culture cannot grow overnight. - (reply: 11)
275. Problem with in vitro transcription - (reply: 1)
276. excess amount of primers - (reply: 2)
277. Dehydrating and Reconstituting primers - (reply: 15)
278. Trouble with PCR of short sequence - (reply: 3)
279. EtBr decontamination of gel doc system - (reply: 2)
280. RNA Precipitation - (reply: 1)
281. filling the digested vector and insert for blunt end cloning - (reply: 2)
282. Identifying CpG islands on a promoter... - (reply: 1)
283. Bacterial DNA extraction/purification from animal tissue - (reply: 1)
284. Help with plasmid extraction and redissolving? - (reply: 5)
285. Thick 100-bp band disappeared under UV - (reply: 3)
286. codon optimization for CHO cells - (reply: 2)
287. Mutation in 3'UTR - (reply: 2)
288. Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
289. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
290. Can you store DNA in loading dye buffer short term? - (reply: 2)
291. Heavy and light Chain - (reply: 9)
292. Troubles with PCR (Rosa26 locus in mouse line) VERY CONFUSING! - (reply: 6)
293. Quick method for DNA extraction using lysis - (reply: 4)
294. Editing out secondary potential ORFs? - (reply: 2)
295. Sterility issues with plasmid reconstitution buffer? - (reply: 3)
296. Heating elution buffer for plasmid prep - (reply: 1)
297. qPCR and miRNA isolation - (reply: 4)
298. Yeast Two Hybrid system - (reply: 7)
299. how much purify protein nedded for IP - (reply: 9)
300. lyophilized protein dilution - (reply: 6)