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271. No amplification during RT-PCR - (reply: 4)
272. rescue cloning of a transposon mutant - (reply: 2)
273. Dephosphorylation - (reply: 7)
274. study of receptors in cell lines - (reply: 1)
275. Mouse genotyping issues - (reply: 1)
276. In-situ hybridization - (reply: 1)
277. His tag introduction in to gene and primer design - (reply: 2)
278. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
279. Principle of silica membrane binding of nucleic acid - (reply: 6)
280. genogroup and genotype - (reply: 2)
281. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
282. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
283. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
284. Beta alanine alternative? - (reply: 2)
285. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
286. lncRNA full-sequence or partial sequence - (reply: 2)
287. Cloning at 6 kb gene - (reply: 3)
288. Trouble with DNA purity (A260/A280) - (reply: 3)
289. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
290. Luciferase assay - extreme variation in RLU - (reply: 1)
291. site-directed mutagenesis - (reply: 20)
292. qPCR detection - (reply: 2)
293. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
294. TCA precipitation - (reply: 1)
295. SOC vs TB in transformations - (reply: 1)
296. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
297. Single RE site cloning - (reply: 3)
298. PCR product running on agarose gel - (reply: 32)
299. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
300. PCR amplification with high fidelity enzyme - (reply: 1)
272. rescue cloning of a transposon mutant - (reply: 2)
273. Dephosphorylation - (reply: 7)
274. study of receptors in cell lines - (reply: 1)
275. Mouse genotyping issues - (reply: 1)
276. In-situ hybridization - (reply: 1)
277. His tag introduction in to gene and primer design - (reply: 2)
278. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
279. Principle of silica membrane binding of nucleic acid - (reply: 6)
280. genogroup and genotype - (reply: 2)
281. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
282. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
283. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
284. Beta alanine alternative? - (reply: 2)
285. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
286. lncRNA full-sequence or partial sequence - (reply: 2)
287. Cloning at 6 kb gene - (reply: 3)
288. Trouble with DNA purity (A260/A280) - (reply: 3)
289. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
290. Luciferase assay - extreme variation in RLU - (reply: 1)
291. site-directed mutagenesis - (reply: 20)
292. qPCR detection - (reply: 2)
293. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
294. TCA precipitation - (reply: 1)
295. SOC vs TB in transformations - (reply: 1)
296. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
297. Single RE site cloning - (reply: 3)
298. PCR product running on agarose gel - (reply: 32)
299. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
300. PCR amplification with high fidelity enzyme - (reply: 1)