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571. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
572. lncRNA full-sequence or partial sequence - (reply: 2)
573. Cloning at 6 kb gene - (reply: 3)
574. Trouble with DNA purity (A260/A280) - (reply: 3)
575. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
576. Luciferase assay - extreme variation in RLU - (reply: 1)
577. site-directed mutagenesis - (reply: 20)
578. qPCR detection - (reply: 2)
579. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
580. TCA precipitation - (reply: 1)
581. SOC vs TB in transformations - (reply: 1)
582. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
583. Single RE site cloning - (reply: 3)
584. PCR product running on agarose gel - (reply: 32)
585. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
586. PCR amplification with high fidelity enzyme - (reply: 1)
587. Transformation problem - (reply: 5)
588. finding gene sequence to delete - (reply: 4)
589. Blue fluorescent color of agarose gel in UV - (reply: 7)
590. heterologous gene integration into e.coli genome - (reply: 1)
591. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
592. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
593. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
594. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
595. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
596. Continuous growth of E. coli on AMP LB plate - (reply: 2)
597. Plasmid compatibly groups - (reply: 5)
598. too much DNA on agarose gel - (reply: 10)
599. What are the "mammalian vectors N1 and C1"? - (reply: 1)
600. Life before BLAST etc? Help needed... - (reply: 1)
572. lncRNA full-sequence or partial sequence - (reply: 2)
573. Cloning at 6 kb gene - (reply: 3)
574. Trouble with DNA purity (A260/A280) - (reply: 3)
575. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
576. Luciferase assay - extreme variation in RLU - (reply: 1)
577. site-directed mutagenesis - (reply: 20)
578. qPCR detection - (reply: 2)
579. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
580. TCA precipitation - (reply: 1)
581. SOC vs TB in transformations - (reply: 1)
582. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
583. Single RE site cloning - (reply: 3)
584. PCR product running on agarose gel - (reply: 32)
585. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
586. PCR amplification with high fidelity enzyme - (reply: 1)
587. Transformation problem - (reply: 5)
588. finding gene sequence to delete - (reply: 4)
589. Blue fluorescent color of agarose gel in UV - (reply: 7)
590. heterologous gene integration into e.coli genome - (reply: 1)
591. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
592. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
593. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
594. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
595. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
596. Continuous growth of E. coli on AMP LB plate - (reply: 2)
597. Plasmid compatibly groups - (reply: 5)
598. too much DNA on agarose gel - (reply: 10)
599. What are the "mammalian vectors N1 and C1"? - (reply: 1)
600. Life before BLAST etc? Help needed... - (reply: 1)