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571. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
572. TCA precipitation - (reply: 1)
573. SOC vs TB in transformations - (reply: 1)
574. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
575. Single RE site cloning - (reply: 3)
576. PCR product running on agarose gel - (reply: 32)
577. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
578. PCR amplification with high fidelity enzyme - (reply: 1)
579. Transformation problem - (reply: 5)
580. finding gene sequence to delete - (reply: 4)
581. Blue fluorescent color of agarose gel in UV - (reply: 7)
582. heterologous gene integration into e.coli genome - (reply: 1)
583. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
584. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
585. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
586. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
587. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
588. Continuous growth of E. coli on AMP LB plate - (reply: 2)
589. Plasmid compatibly groups - (reply: 5)
590. too much DNA on agarose gel - (reply: 10)
591. What are the "mammalian vectors N1 and C1"? - (reply: 1)
592. Life before BLAST etc? Help needed... - (reply: 1)
593. How base pairs upstream of gene to include for the promoter? - (reply: 3)
594. Neomycin resistance sequence - (reply: 1)
595. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
596. polyacrylamide RNA gel - (reply: 4)
597. Making a standard curve from bacterial suspensions - (reply: 1)
598. Is my RNA degraded? - (reply: 7)
599. Phosphotyrosine western blotting, HELP! - (reply: 1)
600. Bacteriophage genome extraction - (reply: 1)
572. TCA precipitation - (reply: 1)
573. SOC vs TB in transformations - (reply: 1)
574. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
575. Single RE site cloning - (reply: 3)
576. PCR product running on agarose gel - (reply: 32)
577. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
578. PCR amplification with high fidelity enzyme - (reply: 1)
579. Transformation problem - (reply: 5)
580. finding gene sequence to delete - (reply: 4)
581. Blue fluorescent color of agarose gel in UV - (reply: 7)
582. heterologous gene integration into e.coli genome - (reply: 1)
583. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
584. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
585. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
586. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
587. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
588. Continuous growth of E. coli on AMP LB plate - (reply: 2)
589. Plasmid compatibly groups - (reply: 5)
590. too much DNA on agarose gel - (reply: 10)
591. What are the "mammalian vectors N1 and C1"? - (reply: 1)
592. Life before BLAST etc? Help needed... - (reply: 1)
593. How base pairs upstream of gene to include for the promoter? - (reply: 3)
594. Neomycin resistance sequence - (reply: 1)
595. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
596. polyacrylamide RNA gel - (reply: 4)
597. Making a standard curve from bacterial suspensions - (reply: 1)
598. Is my RNA degraded? - (reply: 7)
599. Phosphotyrosine western blotting, HELP! - (reply: 1)
600. Bacteriophage genome extraction - (reply: 1)