|
571. Cloning at 6 kb gene - (reply: 3)
572. Trouble with DNA purity (A260/A280) - (reply: 3)
573. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
574. Luciferase assay - extreme variation in RLU - (reply: 1)
575. site-directed mutagenesis - (reply: 20)
576. qPCR detection - (reply: 2)
577. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
578. TCA precipitation - (reply: 1)
579. SOC vs TB in transformations - (reply: 1)
580. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
581. Single RE site cloning - (reply: 3)
582. PCR product running on agarose gel - (reply: 32)
583. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
584. PCR amplification with high fidelity enzyme - (reply: 1)
585. Transformation problem - (reply: 5)
586. finding gene sequence to delete - (reply: 4)
587. Blue fluorescent color of agarose gel in UV - (reply: 7)
588. heterologous gene integration into e.coli genome - (reply: 1)
589. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
590. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
591. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
592. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
593. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
594. Continuous growth of E. coli on AMP LB plate - (reply: 2)
595. Plasmid compatibly groups - (reply: 5)
596. too much DNA on agarose gel - (reply: 10)
597. What are the "mammalian vectors N1 and C1"? - (reply: 1)
598. Life before BLAST etc? Help needed... - (reply: 1)
599. How base pairs upstream of gene to include for the promoter? - (reply: 3)
600. Neomycin resistance sequence - (reply: 1)
572. Trouble with DNA purity (A260/A280) - (reply: 3)
573. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
574. Luciferase assay - extreme variation in RLU - (reply: 1)
575. site-directed mutagenesis - (reply: 20)
576. qPCR detection - (reply: 2)
577. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
578. TCA precipitation - (reply: 1)
579. SOC vs TB in transformations - (reply: 1)
580. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
581. Single RE site cloning - (reply: 3)
582. PCR product running on agarose gel - (reply: 32)
583. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
584. PCR amplification with high fidelity enzyme - (reply: 1)
585. Transformation problem - (reply: 5)
586. finding gene sequence to delete - (reply: 4)
587. Blue fluorescent color of agarose gel in UV - (reply: 7)
588. heterologous gene integration into e.coli genome - (reply: 1)
589. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
590. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
591. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
592. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
593. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
594. Continuous growth of E. coli on AMP LB plate - (reply: 2)
595. Plasmid compatibly groups - (reply: 5)
596. too much DNA on agarose gel - (reply: 10)
597. What are the "mammalian vectors N1 and C1"? - (reply: 1)
598. Life before BLAST etc? Help needed... - (reply: 1)
599. How base pairs upstream of gene to include for the promoter? - (reply: 3)
600. Neomycin resistance sequence - (reply: 1)