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661. MTT: for 5 days - (reply: 1)
662. T7 promoter ending; to add or not to add GGG - (reply: 2)
663. Cytoplasmic mRNA isolation protocol - (reply: 1)
664. BSA in PCR reaction - (reply: 1)
665. Adding T7 promoter sequence to vector - (reply: 2)
666. Can CMV promoter start transcription in prokaryote cells - (reply: 1)
667. Taq vs Phusion polymerases - specificity - (reply: 8)
668. RNA isolation of murine pancreatic islets - poor ratios - (reply: 3)
669. Tagging a gene with HA and FLAG using PCR - (reply: 1)
670. Atypical yeild of RNA - (reply: 1)
671. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
672. DNA EtOH/Sodium Acetate precip over the weekend...still good? - (reply: 2)
673. What happens to DNA in the presence of a chaotropic salt? - (reply: 3)
674. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
675. Problems with RNA extraction from Sorghum anthers/pollen - (reply: 1)
676. pcr product digestion problem - (reply: 4)
677. How to find a promoter and TSS? - (reply: 1)
678. A substitute for Triton-X100 in DNA extraction - (reply: 9)
679. Does Proteinase K digest RNAse in lysis buffer? - (reply: 2)
680. Third round PCR - (reply: 2)
681. how to amplify a fungus which is in the host tissue - (reply: 3)
682. RNA pellet gone in the wrong concentration of ethanol - (reply: 4)
683. Extraction of Ribosomes - (reply: 1)
684. Best method for extraction on low concentration environmental DNA? - (reply: 2)
685. how can we say that point mutations alter synthesis of essential amino acids? - (reply: 5)
686. Nanodrop - (reply: 8)
687. Stable cell line - (reply: 1)
688. How to make tris-saturated chloroform - (reply: 3)
689. Genomic DNA extraction - how to quantify for PCR? - (reply: 9)
690. selection of caspase activity assay - (reply: 1)
662. T7 promoter ending; to add or not to add GGG - (reply: 2)
663. Cytoplasmic mRNA isolation protocol - (reply: 1)
664. BSA in PCR reaction - (reply: 1)
665. Adding T7 promoter sequence to vector - (reply: 2)
666. Can CMV promoter start transcription in prokaryote cells - (reply: 1)
667. Taq vs Phusion polymerases - specificity - (reply: 8)
668. RNA isolation of murine pancreatic islets - poor ratios - (reply: 3)
669. Tagging a gene with HA and FLAG using PCR - (reply: 1)
670. Atypical yeild of RNA - (reply: 1)
671. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
672. DNA EtOH/Sodium Acetate precip over the weekend...still good? - (reply: 2)
673. What happens to DNA in the presence of a chaotropic salt? - (reply: 3)
674. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
675. Problems with RNA extraction from Sorghum anthers/pollen - (reply: 1)
676. pcr product digestion problem - (reply: 4)
677. How to find a promoter and TSS? - (reply: 1)
678. A substitute for Triton-X100 in DNA extraction - (reply: 9)
679. Does Proteinase K digest RNAse in lysis buffer? - (reply: 2)
680. Third round PCR - (reply: 2)
681. how to amplify a fungus which is in the host tissue - (reply: 3)
682. RNA pellet gone in the wrong concentration of ethanol - (reply: 4)
683. Extraction of Ribosomes - (reply: 1)
684. Best method for extraction on low concentration environmental DNA? - (reply: 2)
685. how can we say that point mutations alter synthesis of essential amino acids? - (reply: 5)
686. Nanodrop - (reply: 8)
687. Stable cell line - (reply: 1)
688. How to make tris-saturated chloroform - (reply: 3)
689. Genomic DNA extraction - how to quantify for PCR? - (reply: 9)
690. selection of caspase activity assay - (reply: 1)