661. Help - Troubleshooting cloning into pET28 - all screens positive, but sequencing - (reply: 2)
662. quantifying the activity of a transcription factor - (reply: 3)
663. Explanation of "In-Frame" cloning? - (reply: 6)
664. Purity problems in DNA extracted from stool samples - (reply: 3)
665. Taking Culture Medium Samples for LDH Assay - (reply: 2)
666. Multiplex PCR - (reply: 33)
667. qPCR: CT value of reference gene - (reply: 3)
668. Where can I purchase T2 or T4 phage genomic DNA? - (reply: 2)
669. PCR internal control - (reply: 2)
670. Primers Freeze-Thaw - (reply: 16)
671. Extraction of insect mitochondrial DNA - (reply: 2)
672. Analysis of mutation - (reply: 1)
673. competent cells: CaCl2 autoclave vs filter sterilisation - (reply: 12)
674. SB buffer recipe. - (reply: 5)
675. silent mutations - (reply: 3)
676. primer design using 16s rRNA - (reply: 1)
677. Best ECL detection reagents - (reply: 2)
678. Storing freshly autoclaved LB agar solution? - (reply: 9)
679. Plasmid Purification and Digestion Problem - (reply: 2)
680. DNA extraction from Fin Clips - (reply: 4)
681. Cholesterol Synthesis - (reply: 2)
682. about the storage of marine Vibrios - (reply: 1)
683. What is your preferred Reverse Transcriptase? - (reply: 4)
684. RNA isolation from small numbers of cells - (reply: 6)
685. Problems with Electrophoresis of RNA - (reply: 3)
686. Checking DNase activity - (reply: 3)
687. PCR & Southern blotting - (reply: 1)
688. Phosphoiesterase II Use and Safety - (reply: 1)
689. Lysis method for single human embryonic cell - (reply: 5)
690. Primer Design HELP! - (reply: 3)

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