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1291. Problems with pJET1,2Blunt - (reply: 1)
1292. DH5 Alpha Lambda pir and Conjugation - (reply: 1)
1293. Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
1294. Overexpression plasmid is not expressing protein - (reply: 13)
1295. Why shouldn't you clone using expression bacteria - (reply: 6)
1296. Unstable A260/A230 in RNA isolation - (reply: 2)
1297. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
1298. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
1299. Radioactive calculation - (reply: 3)
1300. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1301. invitro mutagenesis - (reply: 5)
1302. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1303. Extra band on gel after miniprep - (reply: 6)
1304. 3 distinct bands of gDNA on gel - (reply: 2)
1305. PCR Mastermix - (reply: 4)
1306. Unsuccessful self-ligation - (reply: 4)
1307. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1308. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1309. DNA extraction from E. coli - solutions needed? (reply: 6)
1310. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1311. Frequency of restriction enzyme sites in a genome - (reply: 5)
1312. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1313. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1314. Internal Control RNA or DNA - (reply: 4)
1315. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1316. RNA extraction - (reply: 3)
1317. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1318. finding exons for a gene, having the coding sequence only - (reply: 4)
1319. colonies without insert!!! - molecular biology (reply: 1)
1320. Effect of phosphoric acid on a kinase assay - (reply: 2)
1292. DH5 Alpha Lambda pir and Conjugation - (reply: 1)
1293. Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
1294. Overexpression plasmid is not expressing protein - (reply: 13)
1295. Why shouldn't you clone using expression bacteria - (reply: 6)
1296. Unstable A260/A230 in RNA isolation - (reply: 2)
1297. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
1298. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
1299. Radioactive calculation - (reply: 3)
1300. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1301. invitro mutagenesis - (reply: 5)
1302. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1303. Extra band on gel after miniprep - (reply: 6)
1304. 3 distinct bands of gDNA on gel - (reply: 2)
1305. PCR Mastermix - (reply: 4)
1306. Unsuccessful self-ligation - (reply: 4)
1307. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1308. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1309. DNA extraction from E. coli - solutions needed? (reply: 6)
1310. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1311. Frequency of restriction enzyme sites in a genome - (reply: 5)
1312. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1313. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1314. Internal Control RNA or DNA - (reply: 4)
1315. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1316. RNA extraction - (reply: 3)
1317. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1318. finding exons for a gene, having the coding sequence only - (reply: 4)
1319. colonies without insert!!! - molecular biology (reply: 1)
1320. Effect of phosphoric acid on a kinase assay - (reply: 2)