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1291. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1292. Frequency of restriction enzyme sites in a genome - (reply: 5)
1293. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1294. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1295. Internal Control RNA or DNA - (reply: 4)
1296. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1297. RNA extraction - (reply: 3)
1298. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1299. finding exons for a gene, having the coding sequence only - (reply: 4)
1300. colonies without insert!!! - molecular biology (reply: 1)
1301. Effect of phosphoric acid on a kinase assay - (reply: 2)
1302. Proteinase K stock solution - (reply: 3)
1303. Total RNA only produce 1 band in gel - (reply: 2)
1304. Advice on amount of starting template for subcloning - (reply: 3)
1305. New to Gene cloning - Plasmid vector preparation (reply: 2)
1306. PCR for MULTIPLE mutagenesis - (reply: 4)
1307. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
1308. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
1309. Induction strength : IPTG vs lactose - which one is stronger? (reply: 2)
1310. EMSA - probe disappears with nuclear extract - probe works without nuclear extract... (reply: 1)
1311. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
1312. Digested PCR product migrate slower than uncut - (reply: 8)
1313. PCR question - (reply: 2)
1314. cloning a 34kb insert - please suggest good vectors (reply: 1)
1315. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
1316. Do you know good sequencing companies? - Sanger sequencing (reply: 15)
1317. RNA extraction troubleshooting: low 260/230 - (reply: 1)
1318. Illumina sample prep for Multiplexed sequencing... - How does it work? (reply: 3)
1319. RNA in native gel - (reply: 3)
1320. Comment of this cloning strategy - (reply: 3)
1292. Frequency of restriction enzyme sites in a genome - (reply: 5)
1293. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1294. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1295. Internal Control RNA or DNA - (reply: 4)
1296. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1297. RNA extraction - (reply: 3)
1298. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1299. finding exons for a gene, having the coding sequence only - (reply: 4)
1300. colonies without insert!!! - molecular biology (reply: 1)
1301. Effect of phosphoric acid on a kinase assay - (reply: 2)
1302. Proteinase K stock solution - (reply: 3)
1303. Total RNA only produce 1 band in gel - (reply: 2)
1304. Advice on amount of starting template for subcloning - (reply: 3)
1305. New to Gene cloning - Plasmid vector preparation (reply: 2)
1306. PCR for MULTIPLE mutagenesis - (reply: 4)
1307. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
1308. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
1309. Induction strength : IPTG vs lactose - which one is stronger? (reply: 2)
1310. EMSA - probe disappears with nuclear extract - probe works without nuclear extract... (reply: 1)
1311. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
1312. Digested PCR product migrate slower than uncut - (reply: 8)
1313. PCR question - (reply: 2)
1314. cloning a 34kb insert - please suggest good vectors (reply: 1)
1315. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
1316. Do you know good sequencing companies? - Sanger sequencing (reply: 15)
1317. RNA extraction troubleshooting: low 260/230 - (reply: 1)
1318. Illumina sample prep for Multiplexed sequencing... - How does it work? (reply: 3)
1319. RNA in native gel - (reply: 3)
1320. Comment of this cloning strategy - (reply: 3)