Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1291. e coli plate left too long at 37C - very small clones appeared (reply: 4)
1292. Strand specific probe design for Sindbis virus - (reply: 4)
1293. Genomic DNA purity...??? - strange 260/280 and 260/230 ratios (reply: 7)
1294. Problems with pJET1,2Blunt - (reply: 1)
1295. DH5 Alpha Lambda pir and Conjugation - (reply: 1)
1296. Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
1297. Overexpression plasmid is not expressing protein - (reply: 13)
1298. Why shouldn't you clone using expression bacteria - (reply: 6)
1299. Unstable A260/A230 in RNA isolation - (reply: 2)
1300. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
1301. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
1302. Radioactive calculation - (reply: 3)
1303. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1304. invitro mutagenesis - (reply: 5)
1305. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1306. Extra band on gel after miniprep - (reply: 6)
1307. 3 distinct bands of gDNA on gel - (reply: 2)
1308. PCR Mastermix - (reply: 4)
1309. Unsuccessful self-ligation - (reply: 4)
1310. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1311. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1312. DNA extraction from E. coli - solutions needed? (reply: 6)
1313. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1314. Frequency of restriction enzyme sites in a genome - (reply: 5)
1315. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1316. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1317. Internal Control RNA or DNA - (reply: 4)
1318. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1319. RNA extraction - (reply: 3)
1320. mutation using overlapping PCR, what's the success rate? - (reply: 3)