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Top : New Forum Archives (2009-): : Molecular-Biology
1291. PCR Mastermix - (reply: 4)
1292. Unsuccessful self-ligation - (reply: 4)
1293. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1294. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1295. DNA extraction from E. coli - solutions needed? (reply: 6)
1296. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1297. Frequency of restriction enzyme sites in a genome - (reply: 5)
1298. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1299. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1300. Internal Control RNA or DNA - (reply: 4)
1301. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1302. RNA extraction - (reply: 3)
1303. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1304. finding exons for a gene, having the coding sequence only - (reply: 4)
1305. colonies without insert!!! - molecular biology (reply: 1)
1306. Effect of phosphoric acid on a kinase assay - (reply: 2)
1307. Proteinase K stock solution - (reply: 3)
1308. Total RNA only produce 1 band in gel - (reply: 2)
1309. Advice on amount of starting template for subcloning - (reply: 3)
1310. New to Gene cloning - Plasmid vector preparation (reply: 2)
1311. PCR for MULTIPLE mutagenesis - (reply: 4)
1312. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
1313. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
1314. Induction strength : IPTG vs lactose - which one is stronger? (reply: 2)
1315. EMSA - probe disappears with nuclear extract - probe works without nuclear extract... (reply: 1)
1316. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
1317. Digested PCR product migrate slower than uncut - (reply: 8)
1318. PCR question - (reply: 2)
1319. cloning a 34kb insert - please suggest good vectors (reply: 1)
1320. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)