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1. What housekeeper to use with HEK cells? - (reply: 1)
2. using sybergreen to quantify DNA and assess its quality - (reply: 1)
3. Ligation with small insert and large vector - (reply: 6)
4. contamination possibility - (reply: 4)
5. Point mutations in synthesis order of a 2.4 kb nucleotide fragment - (reply: 1)
6. lipoprotein SlyB - (reply: 2)
7. High Molecular Weight DNA extraction and PFGE - (reply: 2)
8. about Socorex pipette - (reply: 2)
9. Sequencing problem and restricition enzyme digestion - (reply: 9)
10. RNA storage time - (reply: 1)
11. Allosteric regulation - (reply: 1)
12. experiments to determine the relationship between proteins - (reply: 4)
13. TRIZOL trouble - (reply: 1)
14. Agarose gel bands gone (for gel extraction) - (reply: 2)
15. What's the chemistry behind flocculation when Qiagen P2 is mixed with N3 - (reply: 1)
16. About Gel supershift - (reply: 1)
17. cell free DNA and maintaining stability - (reply: 3)
18. pcr 10X buffer preparation - (reply: 2)
19. Enhancer Analysis - (reply: 2)
20. how much template do i need for pcr? - (reply: 5)
21. 5'-azacytidine treatment - (reply: 1)
22. leaky promoter - (reply: 1)
23. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
24. SAP dephosphorylation - (reply: 1)
25. Determining in frame in modified vector - (reply: 1)
26. Deleting restriction enzyme sites - (reply: 1)
27. Creating primers to add restriction sites to vector backbone - (reply: 7)
28. cDNA test on 1% agarose gel - (reply: 3)
29. Plasmid isolation from gram positive bacteria - (reply: 2)
30. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
2. using sybergreen to quantify DNA and assess its quality - (reply: 1)
3. Ligation with small insert and large vector - (reply: 6)
4. contamination possibility - (reply: 4)
5. Point mutations in synthesis order of a 2.4 kb nucleotide fragment - (reply: 1)
6. lipoprotein SlyB - (reply: 2)
7. High Molecular Weight DNA extraction and PFGE - (reply: 2)
8. about Socorex pipette - (reply: 2)
9. Sequencing problem and restricition enzyme digestion - (reply: 9)
10. RNA storage time - (reply: 1)
11. Allosteric regulation - (reply: 1)
12. experiments to determine the relationship between proteins - (reply: 4)
13. TRIZOL trouble - (reply: 1)
14. Agarose gel bands gone (for gel extraction) - (reply: 2)
15. What's the chemistry behind flocculation when Qiagen P2 is mixed with N3 - (reply: 1)
16. About Gel supershift - (reply: 1)
17. cell free DNA and maintaining stability - (reply: 3)
18. pcr 10X buffer preparation - (reply: 2)
19. Enhancer Analysis - (reply: 2)
20. how much template do i need for pcr? - (reply: 5)
21. 5'-azacytidine treatment - (reply: 1)
22. leaky promoter - (reply: 1)
23. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
24. SAP dephosphorylation - (reply: 1)
25. Determining in frame in modified vector - (reply: 1)
26. Deleting restriction enzyme sites - (reply: 1)
27. Creating primers to add restriction sites to vector backbone - (reply: 7)
28. cDNA test on 1% agarose gel - (reply: 3)
29. Plasmid isolation from gram positive bacteria - (reply: 2)
30. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)