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Top : New Forum Archives (2009-): : Molecular-Biology
1. DNA extraction - (reply: 1)
2. Viscous plasmids and transfection issues - (reply: 1)
3. Directional RNAseq Library Prep for NGS. How does it work? - (reply: 1)
4. Immunoprecipitation (IP) washes - (reply: 2)
5. YFP vs GFP tag - (reply: 1)
6. prepare competent cells from existing competent cells - (reply: 2)
7. Gene expression question - (reply: 1)
8. Problem with RNA bands on RNA bleach gel - (reply: 1)
9. Free software for DNA fingerprint analysis - GUI based - (reply: 1)
10. pYr-CMV-Kan2 vector - (reply: 1)
11. Odd issue converting mutiplex PCR to single reactions - (reply: 3)
12. What is your favorite method for getting inserts into vectors? - (reply: 3)
13. Possibly a stupid question. - (reply: 3)
14. Dilution help - (reply: 1)
15. Question about CaCl2 and CaCl2 - (reply: 5)
16. Preservation of RNA/cDNA samples - (reply: 1)
17. Cycloheximide concentration - (reply: 2)
18. Cell cycle - (reply: 2)
19. Design of <=40bp primers adding restriction sites and also Gibson Assembly o - (reply: 1)
20. Very low yields for >10kb fragment gel extractions - (reply: 1)
21. DNA extraction with phenol-chloroform - DNA stuck in phenol layer? - (reply: 1)
22. Cloning of big inserts - (reply: 1)
23. Primer design HELP! - (reply: 4)
24. wnt/beta catenin signalling - (reply: 1)
25. Using RNA minikit for DNA extraction - (reply: 4)
26. Getting terrible grainy background in immunofluorescence experiment - (reply: 5)
27. Purification of linearized AT-rich DNA for transfection - (reply: 4)
28. DNA decontamination chemistry - (reply: 1)
29. Why is the smearing becoming different in the polyacrylamide gel than the agaros - (reply: 2)
30. Site directed mutagenesis stopped working. - (reply: 6)