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Top : New Forum Archives (2009-): : Molecular-Biology
2401. RNA extraction using TRIzol - (reply: 19)
2402. dsRNA construct size - (reply: 2)
2403. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
2404. problems with DNase1 footprinting - DNA won't enter a denaturing urea polyacrylamide gel (reply: 2)
2405. TOP10F' - Can they be used for regular vector propagation? (reply: 1)
2406. Gel shift with large DNA fragments (>1 kb) - Can I still do EMSA? (reply: 1)
2407. re-amplification in real-time PCR - (reply: 1)
2408. 3'RACE and the polyA tail - why only one band? (reply: 2)
2409. midi-prep - (reply: 5)
2410. Degenerative primers = multiple products? - (reply: 5)
2411. 96 Well minipreps? - (reply: 1)
2412. DNAse activity in room temperature - (reply: 3)
2413. Reverse transcribing organically contaminated RNA - (reply: 2)
2414. Phylogenetic interpretation - (reply: 1)
2415. Do you lose RNA by quick freezing? - (reply: 5)
2416. obatining full nucelotide sequences for genes - where can i find them (reply: 4)
2417. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
2418. Converting RE sites - (reply: 2)
2419. Can DNA be solubilized in hydrochloric acid? - (reply: 2)
2420. house keeping gene in transgenic mice. - (reply: 1)
2421. RNA cleanup methods (WITHOUT USING KITS!!) - protocol for removing phenol contamination (reply: 5)
2422. Detect gene in stable cell line, - (reply: 4)
2423. in vivo expression technology - IVET (reply: 2)
2424. basic math question - Molarity (reply: 2)
2425. EMSA supershift - increased band intensity? - (reply: 1)
2426. Who can explain this? Weird cloning problem.... - (reply: 6)
2427. RNA extraction - Which is which? (reply: 6)
2428. DNase I digestion - how long the fragments are at the end??? (reply: 3)
2429. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
2430. pIRESneo for mammalian cells transfection - (reply: 4)