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Top : New Forum Archives (2009-): : Molecular-Biology
2191. PCR with long primers - (reply: 3)
2192. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
2193. Gateway cloning - (reply: 8)
2194. RNA Isolation - RNA isolation from human cell lines (reply: 2)
2195. Which red fluorescence is better? - mCherry of dsRed (reply: 2)
2196. Finding corresponding amino acid in DNA sequence - (reply: 1)
2197. Buffer for 5 Kb DNA fragments - (reply: 2)
2198. Pooling doesn't work - make up rna amount of 10ug (reply: 3)
2199. Western Blot for Bacteria - Loading Control (reply: 1)
2200. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
2201. Southern blotting - copy number determination (reply: 1)
2202. RNase: RNA ratio - making dsRNA (reply: 1)
2203. I hate my Klenow! - (reply: 9)
2204. Tris Base Buffer is the same that Trizma??No I think.. - Buffers:Differences between Tris Base and Trizma.. (reply: 3)
2205. Sequencing doubts - (reply: 3)
2206. Help with Northern Blotting - (reply: 4)
2207. ELISA - how can i get Mab for NS1? (reply: 1)
2208. 0.5% crystal violet - how to make (reply: 2)
2209. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
2210. cDNA 260/280 ratio - (reply: 3)
2211. Amino acid substitution a problem? - Phenylalanine to leucine (reply: 3)
2212. What would happen? sequencing question - (reply: 2)
2213. Chemical transfection with Calcium Chloride - anyone used this method and made the 2X HBS? (reply: 6)
2214. About reporting a new splice variant in NCBI - (reply: 1)
2215. foci formation assay - crystal violet (reply: 1)
2216. In situ hybridization - zebrafish (reply: 1)
2217. transient transfection - (reply: 2)
2218. Can I still use 4-year-old Dig-labeled Marker ? - (reply: 4)
2219. Dot Blot Assay - (reply: 2)
2220. plasmid sequence identification problem - (reply: 2)