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Top : New Forum Archives (2009-): : Molecular-Biology
2641. primer design - (reply: 3)
2642. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
2643. DNA purification - long fragments wanted - (reply: 1)
2644. want gene knock out protocol - (reply: 2)
2645. Unidentified DNA bands - (reply: 1)
2646. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
2647. Accidentally digested DNA overnight - still OK? - (reply: 4)
2648. Shelf life of ampicillin in LB media? - (reply: 6)
2649. Make construct so it is only thing that can PCR after transfection - (reply: 2)
2650. Negative flouroscence in real time PCR - (reply: 2)
2651. Remove RNA after DNA mini-prep - (reply: 1)
2652. Minimum number of PCR cycles to see a product? - (reply: 6)
2653. Ligation after SalI & XhoI Digestion - (reply: 3)
2654. Rac1 mutants - (reply: 1)
2655. RNA from Sheep and Bovine blood - Isolation of RNA from bovine and ovine whole blood (reply: 3)
2656. decontamination - decontamination for PCR (reply: 6)
2657. Troubleshoot ARMS PCR - (reply: 2)
2658. Transfer of gene from one vector to another vector - (reply: 2)
2659. Transfection of vectors - (reply: 8)
2660. negative control for PCR - (reply: 1)
2661. Proteinase K to improve RNA extraction yield - (reply: 2)
2662. cloudy phase during RNA extraction with Trizol - (reply: 10)
2663. EtBr solution - (reply: 3)
2664. cDNA versus cRNA for a microarray - (reply: 1)
2665. Capillary DNA Sequencing - (reply: 6)
2666. y pGEMT first then pGEX-2T - (reply: 2)
2667. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
2668. Plasmid replication - Plasmid replication (reply: 13)
2669. silica purification - problem with double bands after silica purification (reply: 1)
2670. Non-Radioactive In vitro Kinase Assay - MPF and MAPK specific (reply: 2)