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Top : New Forum Archives (2009-): : Molecular-Biology
1951. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
1952. PCR not working - overhangs too long? (reply: 3)
1953. What melting temperature to use - (reply: 1)
1954. PFGE Current? - (reply: 1)
1955. Plasmid frameshift problem after clonning - (reply: 2)
1956. Nde1-BamH1 makes me sad - high background despite DIFFERENT methods (reply: 34)
1957. TA cloning necessary? - (reply: 2)
1958. Is DNA an acid because of phosphate group - (reply: 2)
1959. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
1960. colony formation assay - how many cells to seed (reply: 2)
1961. Conversion of primer unit - ug/ul to uM (reply: 4)
1962. mutagenesis by PCR or just use a kit - (reply: 5)
1963. Drying RNA in Speedvac - (reply: 1)
1964. cDNA for qPCR - Help with basics please (reply: 6)
1965. plasmid extraction kit solution composition - wash buffer (reply: 2)
1966. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
1967. Primer Trouble Shooting - (reply: 5)
1968. Proven Primers Stopped working & now leave smear! - (reply: 2)
1969. Expired chemicals - 0.5M EDTA, can be used again after autoclaved? - (reply: 4)
1970. tri reagent not compatible with plastic? - (reply: 1)
1971. DNA Extraction - (reply: 2)
1972. ligation problem - (reply: 6)
1973. TRIzol v. TRI reagent - (reply: 2)
1974. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
1975. Isolation of DNA from Scat - (reply: 2)
1976. How long will cellular resources last - (reply: 1)
1977. PFGE conditions - (reply: 2)
1978. genomic DNA isolation - (reply: 4)
1979. Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
1980. How to find gene homologues? - (reply: 1)