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Top : New Forum Archives (2009-): : Molecular-Biology
1951. DNA quantification: NanoDrop vs. Picogreen - measurement of ChIP Input DNA (reply: 9)
1952. PCR reagent autoclave - (reply: 2)
1953. weird site directed mutagenesis products - (reply: 3)
1954. extraction of viral rna from plant tissue - (reply: 1)
1955. ampicillin for LB agar - (reply: 2)
1956. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
1957. PCR not working - overhangs too long? (reply: 3)
1958. What melting temperature to use - (reply: 1)
1959. PFGE Current? - (reply: 1)
1960. Plasmid frameshift problem after clonning - (reply: 2)
1961. Nde1-BamH1 makes me sad - high background despite DIFFERENT methods (reply: 34)
1962. TA cloning necessary? - (reply: 2)
1963. Is DNA an acid because of phosphate group - (reply: 2)
1964. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
1965. colony formation assay - how many cells to seed (reply: 2)
1966. Conversion of primer unit - ug/ul to uM (reply: 4)
1967. mutagenesis by PCR or just use a kit - (reply: 5)
1968. Drying RNA in Speedvac - (reply: 1)
1969. cDNA for qPCR - Help with basics please (reply: 6)
1970. plasmid extraction kit solution composition - wash buffer (reply: 2)
1971. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
1972. Primer Trouble Shooting - (reply: 5)
1973. Proven Primers Stopped working & now leave smear! - (reply: 2)
1974. Expired chemicals - 0.5M EDTA, can be used again after autoclaved? - (reply: 4)
1975. tri reagent not compatible with plastic? - (reply: 1)
1976. DNA Extraction - (reply: 2)
1977. ligation problem - (reply: 6)
1978. TRIzol v. TRI reagent - (reply: 2)
1979. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
1980. Isolation of DNA from Scat - (reply: 2)