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Top : New Forum Archives (2009-): : Molecular-Biology
1951. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
1952. Genomic DNA - (reply: 2)
1953. No RIN obtained with Bioanalyzer? - (reply: 2)
1954. Sequence, question! - (reply: 8)
1955. PCR failed no product.. help - (reply: 10)
1956. number of molecules in PCR - (reply: 3)
1957. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
1958. EMSA - how to make long probes ? (reply: 4)
1959. point mutation when transformed in BL21 - (reply: 2)
1960. Primer dilution - PCR primer dilution (reply: 2)
1961. DNA quantification: NanoDrop vs. Picogreen - measurement of ChIP Input DNA (reply: 9)
1962. PCR reagent autoclave - (reply: 2)
1963. weird site directed mutagenesis products - (reply: 3)
1964. extraction of viral rna from plant tissue - (reply: 1)
1965. ampicillin for LB agar - (reply: 2)
1966. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
1967. PCR not working - overhangs too long? (reply: 3)
1968. What melting temperature to use - (reply: 1)
1969. PFGE Current? - (reply: 1)
1970. Plasmid frameshift problem after clonning - (reply: 2)
1971. Nde1-BamH1 makes me sad - high background despite DIFFERENT methods (reply: 34)
1972. TA cloning necessary? - (reply: 2)
1973. Is DNA an acid because of phosphate group - (reply: 2)
1974. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
1975. colony formation assay - how many cells to seed (reply: 2)
1976. Conversion of primer unit - ug/ul to uM (reply: 4)
1977. mutagenesis by PCR or just use a kit - (reply: 5)
1978. Drying RNA in Speedvac - (reply: 1)
1979. cDNA for qPCR - Help with basics please (reply: 6)
1980. plasmid extraction kit solution composition - wash buffer (reply: 2)