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Top : New Forum Archives (2009-): : Molecular-Biology
691. Mutagenesis, deletion - what is the best kit/protocol (reply: 6)
692. Ethidium Bromide Stability - (reply: 1)
693. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
694. primer design - microalgae (reply: 2)
695. Trouble to UNDERSTAND gene replacement results - (reply: 1)
696. Would clean dry hot air kill bacteria? - (reply: 6)
697. Genomic DNA contamination in my RNA sample? - (reply: 8)
698. Cloning and expressing a mystery 71KDa protein - (reply: 9)
699. why 12 h culture for plasmid prep? - (reply: 2)
700. Insert magically shortening??? - Cloning issues (reply: 2)
701. Transformation issue - (reply: 1)
702. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
703. PCR yield in ng/ul - (reply: 1)
704. How to add a T7 terminator to a vector? - (reply: 4)
705. Dna after gel extraction, single or double stranded? - (reply: 1)
706. Checking RNA on gel after DIG labeling - (reply: 1)
707. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
708. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
709. DNA-PK inhibitor - Which chemical is the best? (reply: 1)
710. some mathematics help - meaning of 5.0E+03 (reply: 4)
711. Storage conditions - (reply: 4)
712. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
713. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
714. Stability of DNA at RT - (reply: 1)
715. yeast expression (gal1-promotor) - expression in glucose?? (reply: 1)
716. TRIZol Reagent additional reagents & protocol clarifications - (reply: 4)
717. Is it possible to islate strong negative charged protein - (reply: 1)
718. DIG southern blot - (reply: 2)
719. Real time PCR in the presence of heme - (reply: 3)
720. Protein expression vectors Please help - (reply: 2)