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Top : New Forum Archives (2009-): : Molecular-Biology
1381. gDNA restriction digest - (reply: 1)
1382. why most Annexin 5 products are conjugated? - Can't we use it on its own? (reply: 7)
1383. Cloning long fragment...got lots of colonies with vectors that have no inserts.. - (reply: 9)
1384. Promoter "optimization" - (reply: 1)
1385. Adding Restriction Site to DNA - (reply: 3)
1386. how to do gel documentation - (reply: 2)
1387. DNA ext from paraffin-embedded tissue ... a dilemma ! - FFPE tissue refuse to lyse strange whitish clumps formed!! (reply: 1)
1388. Glycine linker - (reply: 2)
1389. Why does the amount of plamids transformed affect colony count? - (reply: 2)
1390. How to obatin intron sequence from cDNA sequence? - (reply: 1)
1391. genomic DNA extraction - why there appears a smear on the gel photo (reply: 11)
1392. Colony Pcr - primers - (reply: 5)
1393. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
1394. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
1395. Sequence size and insert size - Sequence size has larger base pair than insert size (reply: 2)
1396. QCMD samples - (reply: 5)
1397. competent cells - OD600 reading (reply: 6)
1398. Creating overhangs with PCR - (reply: 4)
1399. RNA: Is this degratation or something else? - (reply: 1)
1400. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1401. Is there any software that can be sued to design degenerate primers - (reply: 1)
1402. Primer Decontamination - (reply: 2)
1403. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1404. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1405. How long is "overnight"? - (reply: 3)
1406. Trizol stability - (reply: 1)
1407. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1408. Tube Openers - (reply: 3)
1409. taq man probes non specific? - (reply: 1)
1410. Help! low efficiency of transfection - (reply: 2)