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Top : New Forum Archives (2009-): : Molecular-Biology
1381. purification of PCR product for cloning in a vector - (reply: 3)
1382. elimination of Protein interference from DNA - for RT-PCR (reply: 2)
1383. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
1384. Adapter ligation problem - (reply: 5)
1385. Primer design with a tag - (reply: 4)
1386. Lithium electrophoresis buffer - Why does it not work? (reply: 2)
1387. Weird migration of double digested plasmid - (reply: 4)
1388. Run agarose/formaldehyde gel for 5 mins before loading samples? Why? - (reply: 1)
1389. Myc tags - (reply: 1)
1390. MOPS: How do you sterlise yours? - (reply: 2)
1391. Which gel concentration - (reply: 4)
1392. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
1393. PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
1394. MINI Dialysis Units - below 2K MWCO? (reply: 1)
1395. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
1396. Rotten Fish Smell in TAE Buffer - Stinky TAE (reply: 3)
1397. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
1398. Combining forward and reverse primer gives different size on gel - (reply: 2)
1399. Help for co-transfection of proteins and plasmid - (reply: 1)
1400. PCR of complement genomic DNA - (reply: 3)
1401. RNAi is somatic and germline cells - (reply: 3)
1402. subcloning into bacterial expression vector - (reply: 1)
1403. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
1404. cloning into F1 ori of expression vector??? - (reply: 1)
1405. primers for fungal identification - (reply: 9)
1406. protein overexpression in HeLa cells - (reply: 1)
1407. TBE Acrylamide/Formamide gels running slowly - (reply: 4)
1408. Sequencing promoter regions - (reply: 6)
1409. PCR for long and repetitive region from genomic DNA - (reply: 4)
1410. dna isolation - dna isolation (reply: 1)