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Top : New Forum Archives (2009-): : Molecular-Biology
1261. Run agarose/formaldehyde gel for 5 mins before loading samples? Why? - (reply: 1)
1262. Myc tags - (reply: 1)
1263. MOPS: How do you sterlise yours? - (reply: 2)
1264. Which gel concentration - (reply: 4)
1265. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
1266. PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
1267. MINI Dialysis Units - below 2K MWCO? (reply: 1)
1268. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
1269. Rotten Fish Smell in TAE Buffer - Stinky TAE (reply: 3)
1270. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
1271. Combining forward and reverse primer gives different size on gel - (reply: 2)
1272. Help for co-transfection of proteins and plasmid - (reply: 1)
1273. PCR of complement genomic DNA - (reply: 3)
1274. RNAi is somatic and germline cells - (reply: 3)
1275. subcloning into bacterial expression vector - (reply: 1)
1276. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
1277. cloning into F1 ori of expression vector??? - (reply: 1)
1278. primers for fungal identification - (reply: 9)
1279. protein overexpression in HeLa cells - (reply: 1)
1280. TBE Acrylamide/Formamide gels running slowly - (reply: 4)
1281. Sequencing promoter regions - (reply: 6)
1282. PCR for long and repetitive region from genomic DNA - (reply: 4)
1283. dna isolation - dna isolation (reply: 1)
1284. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
1285. DNA extraction from plated colonies - (reply: 3)
1286. PCR of repeated region - (reply: 1)
1287. Sense and antisense DNA, and primer design - (reply: 6)
1288. negative control in qRT-PCR - (reply: 6)
1289. Separating cut/non-cut vector - (reply: 6)
1290. PCR bands in NTC but NOT in negative samples - (reply: 2)