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Top : New Forum Archives (2009-): : Molecular-Biology
1261. Doing PCR on Nebulized DNA - (reply: 2)
1262. e coli plate left too long at 37C - very small clones appeared (reply: 4)
1263. Strand specific probe design for Sindbis virus - (reply: 4)
1264. Genomic DNA purity...??? - strange 260/280 and 260/230 ratios (reply: 7)
1265. Problems with pJET1,2Blunt - (reply: 1)
1266. DH5 Alpha Lambda pir and Conjugation - (reply: 1)
1267. Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
1268. Overexpression plasmid is not expressing protein - (reply: 13)
1269. Why shouldn't you clone using expression bacteria - (reply: 6)
1270. Unstable A260/A230 in RNA isolation - (reply: 2)
1271. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
1272. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
1273. Radioactive calculation - (reply: 3)
1274. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1275. invitro mutagenesis - (reply: 5)
1276. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1277. Extra band on gel after miniprep - (reply: 6)
1278. 3 distinct bands of gDNA on gel - (reply: 2)
1279. PCR Mastermix - (reply: 4)
1280. Unsuccessful self-ligation - (reply: 4)
1281. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1282. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1283. DNA extraction from E. coli - solutions needed? (reply: 6)
1284. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1285. Frequency of restriction enzyme sites in a genome - (reply: 5)
1286. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1287. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1288. Internal Control RNA or DNA - (reply: 4)
1289. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1290. RNA extraction - (reply: 3)