Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1921. Site directed mutagenesis-PCR - PCR reaction failing (reply: 1)
1922. Need help desperately: Yeast tRNA and hybridization buffer - (reply: 1)
1923. Insert Sequence Problem in Cloning - (reply: 2)
1924. dilution problem - dilution problem (reply: 1)
1925. How do i increase my rna yield - How do i increase my rna yield (reply: 3)
1926. sequencing with forward/reverse primers - (reply: 7)
1927. can klenow fragment be used to remove the 3' overhangs? - (reply: 1)
1928. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
1929. Viewing Gels - (reply: 3)
1930. ccdB survival E. coli transformation - (reply: 2)
1931. how to get higher RNA purity? - (reply: 3)
1932. Snap freezing worm samples for RNA prep - is it essential? (reply: 1)
1933. Adding A overhangs - primer design implications? - (reply: 1)
1934. I can't resolve 2 proteins (LC3-I and II) in Tricine gels - (reply: 5)
1935. Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
1936. Fungal DNA Isolation - Fungal DNA isolation without liquid nitrogen (reply: 1)
1937. Translational Efficiancy - (reply: 1)
1938. Electroporation strange problem - (reply: 1)
1939. reverse transcriptase and cDNA - (reply: 1)
1940. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
1941. stable cell line - (reply: 2)
1942. Site-directed mutagenesis (repeat oligo insert) - Strange mutagenesis reults! (reply: 10)
1943. Low RNA yield after extraction by RNA blood mini kit. - (reply: 2)
1944. Restriction Enzymes - Enzymes from different companies work together? (reply: 2)
1945. Plant RNA Isolation Aid - (reply: 3)
1946. best plasmid purification kit for transfection - (reply: 1)
1947. insert sequence in clone - (reply: 6)
1948. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
1949. how long is carb stable in liquid broth at 37C - I need longer incubation times of bacteria because of the transformant (reply: 1)
1950. Blotting technique principle - (reply: 1)