Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1921. PCR not working - overhangs too long? (reply: 3)
1922. What melting temperature to use - (reply: 1)
1923. PFGE Current? - (reply: 1)
1924. Plasmid frameshift problem after clonning - (reply: 2)
1925. Nde1-BamH1 makes me sad - high background despite DIFFERENT methods (reply: 34)
1926. TA cloning necessary? - (reply: 2)
1927. Is DNA an acid because of phosphate group - (reply: 2)
1928. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
1929. colony formation assay - how many cells to seed (reply: 2)
1930. Conversion of primer unit - ug/ul to uM (reply: 4)
1931. mutagenesis by PCR or just use a kit - (reply: 5)
1932. Drying RNA in Speedvac - (reply: 1)
1933. cDNA for qPCR - Help with basics please (reply: 6)
1934. plasmid extraction kit solution composition - wash buffer (reply: 2)
1935. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
1936. Primer Trouble Shooting - (reply: 5)
1937. Proven Primers Stopped working & now leave smear! - (reply: 2)
1938. Expired chemicals - 0.5M EDTA, can be used again after autoclaved? - (reply: 4)
1939. tri reagent not compatible with plastic? - (reply: 1)
1940. DNA Extraction - (reply: 2)
1941. ligation problem - (reply: 6)
1942. TRIzol v. TRI reagent - (reply: 2)
1943. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
1944. Isolation of DNA from Scat - (reply: 2)
1945. How long will cellular resources last - (reply: 1)
1946. PFGE conditions - (reply: 2)
1947. genomic DNA isolation - (reply: 4)
1948. Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
1949. How to find gene homologues? - (reply: 1)
1950. IHC vs qPCR - is this possible (reply: 1)