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Top : New Forum Archives (2009-): : Molecular-Biology
1921. insert sequence in clone - (reply: 6)
1922. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
1923. how long is carb stable in liquid broth at 37C - I need longer incubation times of bacteria because of the transformant (reply: 1)
1924. Blotting technique principle - (reply: 1)
1925. any one tried in-vitro expression system ? - Roche is the best ? (reply: 1)
1926. Taq at 2-8 - (reply: 4)
1927. cloning in pET 30 B - (reply: 1)
1928. molcecular biology protocols - name of the famous series of books (reply: 2)
1929. cell adhesion assay - collagen (reply: 1)
1930. freezing during RNA isolation step - (reply: 1)
1931. Restriction enzyme buffer running out ? - (reply: 1)
1932. Low 260/230 ratio in my RNA - (reply: 2)
1933. Help for some general DNA Extraction Questions - (reply: 5)
1934. Replacement of Promoter - To replace araBAD promoter to T7 promoter (reply: 6)
1935. Gel loading dye recipe - trying to DIY Qiagen gel loading dye (reply: 4)
1936. how to check the quality of DNA? - (reply: 4)
1937. topoisomerase problem - (reply: 1)
1938. functional analysis of intron mutations - (reply: 7)
1939. 20mer Oligonucleotide running in the wrong direction - (reply: 1)
1940. Gateway on BAC - Perform LR reaction on long constructs (reply: 2)
1941. Can Morpholino oligo be delivered in oocytes by lipofectin - (reply: 2)
1942. Separating PCR product on agarose gel with similar sizes - (reply: 5)
1943. Vector map for pMH1 - Can't find it anywhere! (reply: 2)
1944. DNA precipitation - (reply: 3)
1945. No RNA after Trizol extraction - Lamba max: 230 !!! (reply: 8)
1946. yeast two hybrid - (reply: 1)
1947. MTT - cell prolferation vs apoptosis (reply: 2)
1948. Homogenising tissue, what do you rinse with? - (reply: 4)
1949. Extracting precious tissue from too large of volumes of RNAlater - stored tissue in too large of volume of RNAlater, can't recover (reply: 5)
1950. mRNA Bioanalyzer - (reply: 1)