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Top : New Forum Archives (2009-): : Molecular-Biology
1921. point mutation when transformed in BL21 - (reply: 2)
1922. Primer dilution - PCR primer dilution (reply: 2)
1923. DNA quantification: NanoDrop vs. Picogreen - measurement of ChIP Input DNA (reply: 9)
1924. PCR reagent autoclave - (reply: 2)
1925. weird site directed mutagenesis products - (reply: 3)
1926. extraction of viral rna from plant tissue - (reply: 1)
1927. ampicillin for LB agar - (reply: 2)
1928. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
1929. PCR not working - overhangs too long? (reply: 3)
1930. What melting temperature to use - (reply: 1)
1931. PFGE Current? - (reply: 1)
1932. Plasmid frameshift problem after clonning - (reply: 2)
1933. Nde1-BamH1 makes me sad - high background despite DIFFERENT methods (reply: 34)
1934. TA cloning necessary? - (reply: 2)
1935. Is DNA an acid because of phosphate group - (reply: 2)
1936. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
1937. colony formation assay - how many cells to seed (reply: 2)
1938. Conversion of primer unit - ug/ul to uM (reply: 4)
1939. mutagenesis by PCR or just use a kit - (reply: 5)
1940. Drying RNA in Speedvac - (reply: 1)
1941. cDNA for qPCR - Help with basics please (reply: 6)
1942. plasmid extraction kit solution composition - wash buffer (reply: 2)
1943. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
1944. Primer Trouble Shooting - (reply: 5)
1945. Proven Primers Stopped working & now leave smear! - (reply: 2)
1946. Expired chemicals - 0.5M EDTA, can be used again after autoclaved? - (reply: 4)
1947. tri reagent not compatible with plastic? - (reply: 1)
1948. DNA Extraction - (reply: 2)
1949. ligation problem - (reply: 6)
1950. TRIzol v. TRI reagent - (reply: 2)