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Top : New Forum Archives (2009-): : Molecular-Biology
1921. can klenow fragment be used to remove the 3' overhangs? - (reply: 1)
1922. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
1923. Viewing Gels - (reply: 3)
1924. ccdB survival E. coli transformation - (reply: 2)
1925. how to get higher RNA purity? - (reply: 3)
1926. Snap freezing worm samples for RNA prep - is it essential? (reply: 1)
1927. Adding A overhangs - primer design implications? - (reply: 1)
1928. I can't resolve 2 proteins (LC3-I and II) in Tricine gels - (reply: 5)
1929. Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
1930. Fungal DNA Isolation - Fungal DNA isolation without liquid nitrogen (reply: 1)
1931. Translational Efficiancy - (reply: 1)
1932. Electroporation strange problem - (reply: 1)
1933. reverse transcriptase and cDNA - (reply: 1)
1934. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
1935. stable cell line - (reply: 2)
1936. Site-directed mutagenesis (repeat oligo insert) - Strange mutagenesis reults! (reply: 10)
1937. Low RNA yield after extraction by RNA blood mini kit. - (reply: 2)
1938. Restriction Enzymes - Enzymes from different companies work together? (reply: 2)
1939. Plant RNA Isolation Aid - (reply: 3)
1940. best plasmid purification kit for transfection - (reply: 1)
1941. insert sequence in clone - (reply: 6)
1942. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
1943. how long is carb stable in liquid broth at 37C - I need longer incubation times of bacteria because of the transformant (reply: 1)
1944. Blotting technique principle - (reply: 1)
1945. any one tried in-vitro expression system ? - Roche is the best ? (reply: 1)
1946. Taq at 2-8 - (reply: 4)
1947. cloning in pET 30 B - (reply: 1)
1948. molcecular biology protocols - name of the famous series of books (reply: 2)
1949. cell adhesion assay - collagen (reply: 1)
1950. freezing during RNA isolation step - (reply: 1)