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Top : New Forum Archives (2009-): : Molecular-Biology
1921. PCR reagent autoclave - (reply: 2)
1922. weird site directed mutagenesis products - (reply: 3)
1923. extraction of viral rna from plant tissue - (reply: 1)
1924. ampicillin for LB agar - (reply: 2)
1925. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
1926. PCR not working - overhangs too long? (reply: 3)
1927. What melting temperature to use - (reply: 1)
1928. PFGE Current? - (reply: 1)
1929. Plasmid frameshift problem after clonning - (reply: 2)
1930. Nde1-BamH1 makes me sad - high background despite DIFFERENT methods (reply: 34)
1931. TA cloning necessary? - (reply: 2)
1932. Is DNA an acid because of phosphate group - (reply: 2)
1933. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
1934. colony formation assay - how many cells to seed (reply: 2)
1935. Conversion of primer unit - ug/ul to uM (reply: 4)
1936. mutagenesis by PCR or just use a kit - (reply: 5)
1937. Drying RNA in Speedvac - (reply: 1)
1938. cDNA for qPCR - Help with basics please (reply: 6)
1939. plasmid extraction kit solution composition - wash buffer (reply: 2)
1940. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
1941. Primer Trouble Shooting - (reply: 5)
1942. Proven Primers Stopped working & now leave smear! - (reply: 2)
1943. Expired chemicals - 0.5M EDTA, can be used again after autoclaved? - (reply: 4)
1944. tri reagent not compatible with plastic? - (reply: 1)
1945. DNA Extraction - (reply: 2)
1946. ligation problem - (reply: 6)
1947. TRIzol v. TRI reagent - (reply: 2)
1948. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
1949. Isolation of DNA from Scat - (reply: 2)
1950. How long will cellular resources last - (reply: 1)