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Top : New Forum Archives (2009-): : Molecular-Biology
1111. Primer design - (reply: 3)
1112. Problems for saturation mutagenesis library - (reply: 12)
1113. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
1114. RNA from guinea pig lung=degraded - (reply: 5)
1115. What transcription factors are involved in transient transfection - (reply: 1)
1116. Tissue disruption - for RNA isolation (reply: 2)
1117. concentration - concentration of DNA (reply: 3)
1118. Random digest of double stranded DNA - (reply: 1)
1119. Best transcription vector - (reply: 1)
1120. Problem in picking single colony - (reply: 2)
1121. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
1122. Agarose - (reply: 7)
1123. RNA - extraction and pcr (reply: 1)
1124. Problem with maxiprep - (reply: 2)
1125. selecting drug-resistant colon cancer cells - (reply: 2)
1126. increased mRNA transcript but no activity - (reply: 5)
1127. Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
1128. Gene expression change after mutagenesis-- please help! - (reply: 4)
1129. Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
1130. real-time PCR after restriction enzyme digestion - (reply: 6)
1131. DH5alpha cells - (reply: 4)
1132. DNA extraction of microalgae - microalgae (reply: 3)
1133. gel question - (reply: 3)
1134. Chemiluminescent EMSA - How to develop X-ray film (reply: 3)
1135. EMSA - Digoxygenin labelled kit (reply: 2)
1136. Acquiring cricket paralysis virus - Where can I find some? (reply: 1)
1137. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
1138. DNA probe labeling for EMSA; How? - Long probe, not oligonucletides (reply: 1)
1139. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
1140. DNA extract quality - (reply: 3)