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Top : New Forum Archives (2009-): : Molecular-Biology
1111. Cloning and expressing a mystery 71KDa protein - (reply: 9)
1112. why 12 h culture for plasmid prep? - (reply: 2)
1113. Insert magically shortening??? - Cloning issues (reply: 2)
1114. Transformation issue - (reply: 1)
1115. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
1116. PCR yield in ng/ul - (reply: 1)
1117. How to add a T7 terminator to a vector? - (reply: 4)
1118. Dna after gel extraction, single or double stranded? - (reply: 1)
1119. Checking RNA on gel after DIG labeling - (reply: 1)
1120. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
1121. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
1122. DNA-PK inhibitor - Which chemical is the best? (reply: 1)
1123. some mathematics help - meaning of 5.0E+03 (reply: 4)
1124. Storage conditions - (reply: 4)
1125. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
1126. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
1127. Stability of DNA at RT - (reply: 1)
1128. yeast expression (gal1-promotor) - expression in glucose?? (reply: 1)
1129. TRIZol Reagent additional reagents & protocol clarifications - (reply: 4)
1130. Is it possible to islate strong negative charged protein - (reply: 1)
1131. DIG southern blot - (reply: 2)
1132. Real time PCR in the presence of heme - (reply: 3)
1133. Protein expression vectors Please help - (reply: 2)
1134. removal of residual ethanol - wht to do if i have ETOH in my eluate? (reply: 2)
1135. Loss of RNA conc. and quality in Qiagen RNeasy Protocol - (reply: 4)
1136. issues in PCR amplification - (reply: 2)
1137. extraa bases before RE site - Reply urgent!! (reply: 1)
1138. vectors please help - vectors cleavage sites (reply: 1)
1139. siRNA transfection advise - Need some help on siRNA knockdowns (reply: 1)
1140. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)