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1111. Miniprep OD beyond 2.00 - Troubleshooting-help desperately needed - (reply: 10)
1112. DNA polymerase: recombinant or native? - (reply: 3)
1113. smearing in western blotting - (reply: 4)
1114. CTAB extraction problem - CTAB extraction problem (reply: 2)
1115. Lentiviral Vector - Lentiviral Vector (reply: 5)
1116. How to remove the background and unspecific bands - (reply: 12)
1117. Low Miniprep Yield - (reply: 1)
1118. Primer design - (reply: 3)
1119. Problems for saturation mutagenesis library - (reply: 12)
1120. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
1121. RNA from guinea pig lung=degraded - (reply: 5)
1122. What transcription factors are involved in transient transfection - (reply: 1)
1123. Tissue disruption - for RNA isolation (reply: 2)
1124. concentration - concentration of DNA (reply: 3)
1125. Random digest of double stranded DNA - (reply: 1)
1126. Best transcription vector - (reply: 1)
1127. Problem in picking single colony - (reply: 2)
1128. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
1129. Agarose - (reply: 7)
1130. RNA - extraction and pcr (reply: 1)
1131. Problem with maxiprep - (reply: 2)
1132. selecting drug-resistant colon cancer cells - (reply: 2)
1133. increased mRNA transcript but no activity - (reply: 5)
1134. Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
1135. Gene expression change after mutagenesis-- please help! - (reply: 4)
1136. Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
1137. real-time PCR after restriction enzyme digestion - (reply: 6)
1138. DH5alpha cells - (reply: 4)
1139. DNA extraction of microalgae - microalgae (reply: 3)
1140. gel question - (reply: 3)
1112. DNA polymerase: recombinant or native? - (reply: 3)
1113. smearing in western blotting - (reply: 4)
1114. CTAB extraction problem - CTAB extraction problem (reply: 2)
1115. Lentiviral Vector - Lentiviral Vector (reply: 5)
1116. How to remove the background and unspecific bands - (reply: 12)
1117. Low Miniprep Yield - (reply: 1)
1118. Primer design - (reply: 3)
1119. Problems for saturation mutagenesis library - (reply: 12)
1120. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
1121. RNA from guinea pig lung=degraded - (reply: 5)
1122. What transcription factors are involved in transient transfection - (reply: 1)
1123. Tissue disruption - for RNA isolation (reply: 2)
1124. concentration - concentration of DNA (reply: 3)
1125. Random digest of double stranded DNA - (reply: 1)
1126. Best transcription vector - (reply: 1)
1127. Problem in picking single colony - (reply: 2)
1128. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
1129. Agarose - (reply: 7)
1130. RNA - extraction and pcr (reply: 1)
1131. Problem with maxiprep - (reply: 2)
1132. selecting drug-resistant colon cancer cells - (reply: 2)
1133. increased mRNA transcript but no activity - (reply: 5)
1134. Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
1135. Gene expression change after mutagenesis-- please help! - (reply: 4)
1136. Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
1137. real-time PCR after restriction enzyme digestion - (reply: 6)
1138. DH5alpha cells - (reply: 4)
1139. DNA extraction of microalgae - microalgae (reply: 3)
1140. gel question - (reply: 3)