Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1111. Blunt end cloning of 4Kb product - (reply: 3)
1112. Getting Rid of DNA after Transcription - (reply: 1)
1113. expression problem - (reply: 2)
1114. DNA migrate differently in agarose vs PAGE gel - (reply: 4)
1115. Make additional cycles with an already finished pcr product - (reply: 3)
1116. DNA Concentration - (reply: 1)
1117. Co-immunoprecipitation - (reply: 1)
1118. Miniprep OD beyond 2.00 - Troubleshooting-help desperately needed - (reply: 10)
1119. DNA polymerase: recombinant or native? - (reply: 3)
1120. smearing in western blotting - (reply: 4)
1121. CTAB extraction problem - CTAB extraction problem (reply: 2)
1122. Lentiviral Vector - Lentiviral Vector (reply: 5)
1123. How to remove the background and unspecific bands - (reply: 12)
1124. Low Miniprep Yield - (reply: 1)
1125. Primer design - (reply: 3)
1126. Problems for saturation mutagenesis library - (reply: 12)
1127. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
1128. RNA from guinea pig lung=degraded - (reply: 5)
1129. What transcription factors are involved in transient transfection - (reply: 1)
1130. Tissue disruption - for RNA isolation (reply: 2)
1131. concentration - concentration of DNA (reply: 3)
1132. Random digest of double stranded DNA - (reply: 1)
1133. Best transcription vector - (reply: 1)
1134. Problem in picking single colony - (reply: 2)
1135. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
1136. Agarose - (reply: 7)
1137. RNA - extraction and pcr (reply: 1)
1138. Problem with maxiprep - (reply: 2)
1139. selecting drug-resistant colon cancer cells - (reply: 2)
1140. increased mRNA transcript but no activity - (reply: 5)