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Top : New Forum Archives (2009-): : Molecular-Biology
1651. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
1652. Amount of DNase to use (Qiagen) - (reply: 7)
1653. COMPLETE RNA degradation - (reply: 18)
1654. HpaI blunt end cloning - (reply: 1)
1655. undesired products in multiplex PCR - (reply: 3)
1656. Generate Supercoil Plasmid - it just a thought... (reply: 5)
1657. help with protein localization study using GFP - (reply: 5)
1658. Does a viral gene have introns? - (reply: 4)
1659. Selecting Lethal Mutants? - (reply: 1)
1660. RNA extraction from stained cells - (reply: 2)
1661. agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
1662. No band after digestion - (reply: 4)
1663. Problem with ligation - Insert into the "wrong" vector (reply: 11)
1664. finding promoter - (reply: 1)
1665. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
1666. Cloning in pCR2.1 plasmid - (reply: 7)
1667. How long can DNA precipitate at -20? - (reply: 6)
1668. Nucleotides with different font size - (reply: 2)
1669. primer sequence problem - (reply: 2)
1670. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
1671. lambda phage library - (reply: 1)
1672. More colonies fron vector only control? - (reply: 1)
1673. Mutagenesis again - (reply: 1)
1674. standard curve method for Q-PCR - (reply: 3)
1675. PCR - No Band formation - (reply: 3)
1676. GEL SMEAR - (reply: 4)
1677. Cloning a 3.5Kb fragment for sequencing - P-gem T-easy. (reply: 2)
1678. Localization of kanamycin resistance protein - (reply: 1)
1679. dna sequencing - (reply: 1)
1680. Biotinylated oligo separation - (reply: 4)