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Top : New Forum Archives (2009-): : Molecular-Biology
1651. Transformation background problem - high background in negative control (reply: 9)
1652. Akrylamide Gels - (reply: 2)
1653. Does unautoclaved DEPC inhibit northern blotting? - (reply: 2)
1654. Miniprep and sequencing - (reply: 4)
1655. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
1656. Protein TAG for affinity purification - (reply: 1)
1657. Blunt-Cohesive ligation - Should I change the conditions? (reply: 2)
1658. GFP fused proteins - i'm going to cry... (reply: 3)
1659. what's the meaning? - HEAP (reply: 2)
1660. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
1661. Making gels in advance - (reply: 2)
1662. Phenol-chloroform problem - (reply: 1)
1663. Cloning <100 bp fragments into a plasmid - (reply: 7)
1664. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
1665. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
1666. Amount of DNase to use (Qiagen) - (reply: 7)
1667. COMPLETE RNA degradation - (reply: 18)
1668. HpaI blunt end cloning - (reply: 1)
1669. undesired products in multiplex PCR - (reply: 3)
1670. Generate Supercoil Plasmid - it just a thought... (reply: 5)
1671. help with protein localization study using GFP - (reply: 5)
1672. Does a viral gene have introns? - (reply: 4)
1673. Selecting Lethal Mutants? - (reply: 1)
1674. RNA extraction from stained cells - (reply: 2)
1675. agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
1676. No band after digestion - (reply: 4)
1677. Problem with ligation - Insert into the "wrong" vector (reply: 11)
1678. finding promoter - (reply: 1)
1679. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
1680. Cloning in pCR2.1 plasmid - (reply: 7)