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Top : New Forum Archives (2009-): : Molecular-Biology
2551. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
2552. no band in gel - gel electrophoresis (reply: 1)
2553. stratagene site directed mutagenesis - deletion of single base nucleotide (reply: 3)
2554. How to increase A260/280 and A260/230 ratio for microarray experiment? - (reply: 8)
2555. western blot stripping buffer - please recomend a stripping buffer for re-bloting (reply: 2)
2556. T7 and T7lac - (reply: 1)
2557. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
2558. Primer stock confusion - (reply: 6)
2559. Pancreas RNA extraction - improve protocol needed (reply: 2)
2560. Genomic DNA contamination in RNA sample - (reply: 2)
2561. Isolation DNA and protein from a single sample - (reply: 5)
2562. lipofectamine - (reply: 3)
2563. Gel Analysis Software - which to use (reply: 3)
2564. DNase I digestion - check if it worked?? (reply: 4)
2565. Uniform high backgroud in DIG Sounthern Blot - (reply: 2)
2566. elution of dna from gel - (reply: 5)
2567. What is in the Blocking solution for DIG-Southern Blot? - (reply: 2)
2568. In vitro Transcription kit recommendations? - (reply: 5)
2569. GFP reporter gene - (reply: 3)
2570. Possible to lose a pGEM insert ? - (reply: 2)
2571. PROTEIN PRECIPITATION SOLUTION - (reply: 1)
2572. cloning - How to calculate the insert and linear plsmid concentration for ligati (reply: 1)
2573. PFGE gel - Can I reuse pulsed field certified gel for PFGE? (reply: 2)
2574. Validating real time pcr primers - (reply: 2)
2575. How to find catalytic domain using cDNA - (reply: 2)
2576. low plasmid yield from midi preps - (reply: 4)
2577. Measure nucleotides - (reply: 3)
2578. Proteinase K Protection Assay Conditions - (reply: 2)
2579. no 18S band on RNA agarose gel - (reply: 1)
2580. b-mercaptoethanol in lysis buffer - (reply: 1)