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Top : New Forum Archives (2009-): : Molecular-Biology
2551. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
2552. pIRESneo for mammalian cells transfection - (reply: 4)
2553. H9C2 - cell line - Need clarification (reply: 1)
2554. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
2555. no band in gel - gel electrophoresis (reply: 1)
2556. stratagene site directed mutagenesis - deletion of single base nucleotide (reply: 3)
2557. How to increase A260/280 and A260/230 ratio for microarray experiment? - (reply: 8)
2558. western blot stripping buffer - please recomend a stripping buffer for re-bloting (reply: 2)
2559. T7 and T7lac - (reply: 1)
2560. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
2561. Primer stock confusion - (reply: 6)
2562. Pancreas RNA extraction - improve protocol needed (reply: 2)
2563. Genomic DNA contamination in RNA sample - (reply: 2)
2564. Isolation DNA and protein from a single sample - (reply: 5)
2565. lipofectamine - (reply: 3)
2566. Gel Analysis Software - which to use (reply: 3)
2567. DNase I digestion - check if it worked?? (reply: 4)
2568. Uniform high backgroud in DIG Sounthern Blot - (reply: 2)
2569. elution of dna from gel - (reply: 5)
2570. What is in the Blocking solution for DIG-Southern Blot? - (reply: 2)
2571. In vitro Transcription kit recommendations? - (reply: 5)
2572. GFP reporter gene - (reply: 3)
2573. Possible to lose a pGEM insert ? - (reply: 2)
2574. PROTEIN PRECIPITATION SOLUTION - (reply: 1)
2575. cloning - How to calculate the insert and linear plsmid concentration for ligati (reply: 1)
2576. PFGE gel - Can I reuse pulsed field certified gel for PFGE? (reply: 2)
2577. Validating real time pcr primers - (reply: 2)
2578. How to find catalytic domain using cDNA - (reply: 2)
2579. low plasmid yield from midi preps - (reply: 4)
2580. Measure nucleotides - (reply: 3)