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Top : New Forum Archives (2009-): : Molecular-Biology
2551. Precipitation in NaOH on shelf - (reply: 3)
2552. long PCR primer - (reply: 7)
2553. stable 3t3l1 transfection:serious help needed!!! - (reply: 10)
2554. methods to detect EGFP expression ?! - we have a big problem! (reply: 10)
2555. mutation study - DNA or mRNA sequence (reply: 1)
2556. how to remove a restricted endonuclease site - (reply: 3)
2557. substractive hybridization - (reply: 2)
2558. sequencing problem after pcr - (reply: 4)
2559. Amplifying plasmid - non specific binding of primer - (reply: 3)
2560. DNA folding to form the loop - (reply: 2)
2561. primer design - (reply: 3)
2562. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
2563. DNA purification - long fragments wanted - (reply: 1)
2564. want gene knock out protocol - (reply: 2)
2565. Unidentified DNA bands - (reply: 1)
2566. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
2567. Accidentally digested DNA overnight - still OK? - (reply: 4)
2568. Shelf life of ampicillin in LB media? - (reply: 6)
2569. Make construct so it is only thing that can PCR after transfection - (reply: 2)
2570. Negative flouroscence in real time PCR - (reply: 2)
2571. Remove RNA after DNA mini-prep - (reply: 1)
2572. Minimum number of PCR cycles to see a product? - (reply: 6)
2573. Ligation after SalI & XhoI Digestion - (reply: 3)
2574. Rac1 mutants - (reply: 1)
2575. RNA from Sheep and Bovine blood - Isolation of RNA from bovine and ovine whole blood (reply: 3)
2576. decontamination - decontamination for PCR (reply: 6)
2577. Troubleshoot ARMS PCR - (reply: 2)
2578. Transfer of gene from one vector to another vector - (reply: 2)
2579. Transfection of vectors - (reply: 8)
2580. negative control for PCR - (reply: 1)