Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
2551. TOP10F' - Can they be used for regular vector propagation? (reply: 1)
2552. Gel shift with large DNA fragments (>1 kb) - Can I still do EMSA? (reply: 1)
2553. re-amplification in real-time PCR - (reply: 1)
2554. 3'RACE and the polyA tail - why only one band? (reply: 2)
2555. midi-prep - (reply: 5)
2556. Degenerative primers = multiple products? - (reply: 5)
2557. 96 Well minipreps? - (reply: 1)
2558. DNAse activity in room temperature - (reply: 3)
2559. Reverse transcribing organically contaminated RNA - (reply: 2)
2560. Phylogenetic interpretation - (reply: 1)
2561. Do you lose RNA by quick freezing? - (reply: 5)
2562. obatining full nucelotide sequences for genes - where can i find them (reply: 4)
2563. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
2564. Converting RE sites - (reply: 2)
2565. Can DNA be solubilized in hydrochloric acid? - (reply: 2)
2566. house keeping gene in transgenic mice. - (reply: 1)
2567. RNA cleanup methods (WITHOUT USING KITS!!) - protocol for removing phenol contamination (reply: 5)
2568. Detect gene in stable cell line, - (reply: 4)
2569. in vivo expression technology - IVET (reply: 2)
2570. basic math question - Molarity (reply: 2)
2571. EMSA supershift - increased band intensity? - (reply: 1)
2572. Who can explain this? Weird cloning problem.... - (reply: 6)
2573. RNA extraction - Which is which? (reply: 6)
2574. DNase I digestion - how long the fragments are at the end??? (reply: 3)
2575. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
2576. pIRESneo for mammalian cells transfection - (reply: 4)
2577. H9C2 - cell line - Need clarification (reply: 1)
2578. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
2579. no band in gel - gel electrophoresis (reply: 1)
2580. stratagene site directed mutagenesis - deletion of single base nucleotide (reply: 3)