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Top : New Forum Archives (2009-): : Molecular-Biology
2251. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
2252. PCR with long primers - (reply: 3)
2253. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
2254. Gateway cloning - (reply: 8)
2255. RNA Isolation - RNA isolation from human cell lines (reply: 2)
2256. Which red fluorescence is better? - mCherry of dsRed (reply: 2)
2257. Finding corresponding amino acid in DNA sequence - (reply: 1)
2258. Buffer for 5 Kb DNA fragments - (reply: 2)
2259. Pooling doesn't work - make up rna amount of 10ug (reply: 3)
2260. Western Blot for Bacteria - Loading Control (reply: 1)
2261. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
2262. Southern blotting - copy number determination (reply: 1)
2263. RNase: RNA ratio - making dsRNA (reply: 1)
2264. I hate my Klenow! - (reply: 9)
2265. Tris Base Buffer is the same that Trizma??No I think.. - Buffers:Differences between Tris Base and Trizma.. (reply: 3)
2266. Sequencing doubts - (reply: 3)
2267. Help with Northern Blotting - (reply: 4)
2268. ELISA - how can i get Mab for NS1? (reply: 1)
2269. 0.5% crystal violet - how to make (reply: 2)
2270. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
2271. cDNA 260/280 ratio - (reply: 3)
2272. Amino acid substitution a problem? - Phenylalanine to leucine (reply: 3)
2273. What would happen? sequencing question - (reply: 2)
2274. Chemical transfection with Calcium Chloride - anyone used this method and made the 2X HBS? (reply: 6)
2275. About reporting a new splice variant in NCBI - (reply: 1)
2276. foci formation assay - crystal violet (reply: 1)
2277. In situ hybridization - zebrafish (reply: 1)
2278. transient transfection - (reply: 2)
2279. Can I still use 4-year-old Dig-labeled Marker ? - (reply: 4)
2280. Dot Blot Assay - (reply: 2)