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Top : New Forum Archives (2009-): : Molecular-Biology
1891. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
1892. Normalizing RNA concentrations prior to cDNA production - qPCR and making cDNA (reply: 1)
1893. precipitation of secreted protein from growth media - (reply: 1)
1894. EMSA gel problem - (reply: 2)
1895. cloning - (reply: 6)
1896. Generating stable cell line - (reply: 2)
1897. Can't get clean RNA prep with TRIzol - (reply: 2)
1898. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
1899. vectors maps - (reply: 2)
1900. Fusion of reporter genes - (reply: 2)
1901. reuse electroporation cuvette? - (reply: 4)
1902. PCR Master mix - (reply: 1)
1903. Fake positive colony in lamda RED reconbination, why? - (reply: 2)
1904. Cloning from cDNA - the wrong gene?! - (reply: 2)
1905. Ligation - (reply: 3)
1906. circular or linear pDNA for stable clone generation? - (reply: 1)
1907. failure PCR amplification from low GC content gene - (reply: 5)
1908. recombineering for ES cell targeting - retrieval problems (reply: 1)
1909. different PCR primers for real-time and classic PCR - (reply: 3)
1910. Simple gel question - (reply: 7)
1911. Can I redo a linearization - (reply: 1)
1912. primers deconamination??? - (reply: 6)
1913. Help in SDS PAGE - Molecular Biology (reply: 3)
1914. Ligation Problems - (reply: 2)
1915. JM106 - (reply: 2)
1916. amplified proviral genome and got human sequence - totally different sequences from what i expected (reply: 23)
1917. DNA agarose gel electrophoresis - sum1 can teach me interpret this gel image.. (reply: 3)
1918. Antibiotic resistance genes in a plasmid - How are they expressed? (reply: 3)
1919. Sodium borate buffer - (reply: 2)
1920. primer check!!! - (reply: 6)