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Top : New Forum Archives (2009-): : Molecular-Biology
1891. Blood specimen banking for biomarker - Storing blood specimen for future use (reply: 4)
1892. help with transformation efficiency calculation! - (reply: 1)
1893. Precipitation in DNAse I incubation buffer - (reply: 1)
1894. Taq enzyme works but other enzyme didn't work - (reply: 5)
1895. DNA purification - DNA purification dissolved in water (reply: 3)
1896. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
1897. PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
1898. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
1899. Double digestion using EcoRI and HindIII with pHAT-CAT - (reply: 4)
1900. dh5 alpha competent cells? - (reply: 2)
1901. Hypoxia - downregulated HIF-1A - why me??? (reply: 2)
1902. Bioanalyzer not recognising the chip - chip misaligned/manufacturing problem? - (reply: 4)
1903. transient transfection - (reply: 1)
1904. Bad sequence - (reply: 5)
1905. Southern Blot troubleshooting - Please help (reply: 3)
1906. Removing sequencing migration artefacts - (reply: 1)
1907. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
1908. Probes design for southern-blot - need more information for probes design for southern-blot (reply: 3)
1909. finding promoter - (reply: 1)
1910. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
1911. Normalizing RNA concentrations prior to cDNA production - qPCR and making cDNA (reply: 1)
1912. precipitation of secreted protein from growth media - (reply: 1)
1913. EMSA gel problem - (reply: 2)
1914. cloning - (reply: 6)
1915. Generating stable cell line - (reply: 2)
1916. Can't get clean RNA prep with TRIzol - (reply: 2)
1917. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
1918. vectors maps - (reply: 2)
1919. Fusion of reporter genes - (reply: 2)
1920. reuse electroporation cuvette? - (reply: 4)