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1891. DNA purification - DNA purification dissolved in water (reply: 3)
1892. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
1893. PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
1894. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
1895. Double digestion using EcoRI and HindIII with pHAT-CAT - (reply: 4)
1896. dh5 alpha competent cells? - (reply: 2)
1897. Hypoxia - downregulated HIF-1A - why me??? (reply: 2)
1898. Bioanalyzer not recognising the chip - chip misaligned/manufacturing problem? - (reply: 4)
1899. transient transfection - (reply: 1)
1900. Bad sequence - (reply: 5)
1901. Southern Blot troubleshooting - Please help (reply: 3)
1902. Removing sequencing migration artefacts - (reply: 1)
1903. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
1904. Probes design for southern-blot - need more information for probes design for southern-blot (reply: 3)
1905. finding promoter - (reply: 1)
1906. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
1907. Normalizing RNA concentrations prior to cDNA production - qPCR and making cDNA (reply: 1)
1908. precipitation of secreted protein from growth media - (reply: 1)
1909. EMSA gel problem - (reply: 2)
1910. cloning - (reply: 6)
1911. Generating stable cell line - (reply: 2)
1912. Can't get clean RNA prep with TRIzol - (reply: 2)
1913. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
1914. vectors maps - (reply: 2)
1915. Fusion of reporter genes - (reply: 2)
1916. reuse electroporation cuvette? - (reply: 4)
1917. PCR Master mix - (reply: 1)
1918. Fake positive colony in lamda RED reconbination, why? - (reply: 2)
1919. Cloning from cDNA - the wrong gene?! - (reply: 2)
1920. Ligation - (reply: 3)
1892. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
1893. PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
1894. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
1895. Double digestion using EcoRI and HindIII with pHAT-CAT - (reply: 4)
1896. dh5 alpha competent cells? - (reply: 2)
1897. Hypoxia - downregulated HIF-1A - why me??? (reply: 2)
1898. Bioanalyzer not recognising the chip - chip misaligned/manufacturing problem? - (reply: 4)
1899. transient transfection - (reply: 1)
1900. Bad sequence - (reply: 5)
1901. Southern Blot troubleshooting - Please help (reply: 3)
1902. Removing sequencing migration artefacts - (reply: 1)
1903. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
1904. Probes design for southern-blot - need more information for probes design for southern-blot (reply: 3)
1905. finding promoter - (reply: 1)
1906. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
1907. Normalizing RNA concentrations prior to cDNA production - qPCR and making cDNA (reply: 1)
1908. precipitation of secreted protein from growth media - (reply: 1)
1909. EMSA gel problem - (reply: 2)
1910. cloning - (reply: 6)
1911. Generating stable cell line - (reply: 2)
1912. Can't get clean RNA prep with TRIzol - (reply: 2)
1913. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
1914. vectors maps - (reply: 2)
1915. Fusion of reporter genes - (reply: 2)
1916. reuse electroporation cuvette? - (reply: 4)
1917. PCR Master mix - (reply: 1)
1918. Fake positive colony in lamda RED reconbination, why? - (reply: 2)
1919. Cloning from cDNA - the wrong gene?! - (reply: 2)
1920. Ligation - (reply: 3)