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Top : New Forum Archives (2009-): : Molecular-Biology
1771. PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
1772. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
1773. Double digestion using EcoRI and HindIII with pHAT-CAT - (reply: 4)
1774. dh5 alpha competent cells? - (reply: 2)
1775. Hypoxia - downregulated HIF-1A - why me??? (reply: 2)
1776. Bioanalyzer not recognising the chip - chip misaligned/manufacturing problem? - (reply: 4)
1777. transient transfection - (reply: 1)
1778. Bad sequence - (reply: 5)
1779. Southern Blot troubleshooting - Please help (reply: 3)
1780. Removing sequencing migration artefacts - (reply: 1)
1781. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
1782. Probes design for southern-blot - need more information for probes design for southern-blot (reply: 3)
1783. finding promoter - (reply: 1)
1784. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
1785. Normalizing RNA concentrations prior to cDNA production - qPCR and making cDNA (reply: 1)
1786. precipitation of secreted protein from growth media - (reply: 1)
1787. EMSA gel problem - (reply: 2)
1788. cloning - (reply: 6)
1789. Generating stable cell line - (reply: 2)
1790. Can't get clean RNA prep with TRIzol - (reply: 2)
1791. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
1792. vectors maps - (reply: 2)
1793. Fusion of reporter genes - (reply: 2)
1794. reuse electroporation cuvette? - (reply: 4)
1795. PCR Master mix - (reply: 1)
1796. Fake positive colony in lamda RED reconbination, why? - (reply: 2)
1797. Cloning from cDNA - the wrong gene?! - (reply: 2)
1798. Ligation - (reply: 3)
1799. circular or linear pDNA for stable clone generation? - (reply: 1)
1800. failure PCR amplification from low GC content gene - (reply: 5)