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Top : New Forum Archives (2009-): : Molecular-Biology
1981. Isolation of protein crosslinked to plasmid - (reply: 3)
1982. Inseparable insert in gel purification of the vector - (reply: 3)
1983. Reversible block, 3' OH? - (reply: 2)
1984. pcDNA3-FLAG-p53 Sequence - (reply: 6)
1985. cloning - how to clone a gene having stop codons (reply: 4)
1986. Unhappy bands - (reply: 3)
1987. dam methylated DNA in dam - straights - (reply: 1)
1988. have problems with electroporation transformation of PCP20 into BL21 de3 - (reply: 2)
1989. subcloning problem - inframe fusion (reply: 1)
1990. SSCP - (reply: 2)
1991. OD reading - (reply: 1)
1992. E coli transformation - (reply: 8)
1993. RNAsin overdose! - (reply: 1)
1994. Gel loading dye : sample ratio?? - have to follow strictly? (reply: 6)
1995. how to intentionally alter gene sequence - (reply: 1)
1996. molecular biology - (reply: 2)
1997. How to remove diphosphate from in vitro transcribed RNA? - (reply: 1)
1998. Experience with YM-30 microcon columns from Millipore - (reply: 2)
1999. DNA stains - pronunciation question (reply: 3)
2000. Floating white pellet in miniprep - (reply: 2)
2001. Gel electrophoresis problem - (reply: 7)
2002. Making competent cell from freeze-thawed stock - (reply: 2)
2003. Fluorescent measurements-AMCA - (reply: 4)
2004. northern prob - (reply: 4)
2005. dna smear in electrophoresis - (reply: 6)
2006. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
2007. Reverse-transcription of UV-damaged RNA - (reply: 3)
2008. How could I express two genes in opposite orientation - (reply: 2)
2009. shRNA oligo annealing problem - (reply: 5)
2010. Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)