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Top : New Forum Archives (2009-): : Molecular-Biology
1981. point mutation when transformed in BL21 - (reply: 2)
1982. Primer dilution - PCR primer dilution (reply: 2)
1983. DNA quantification: NanoDrop vs. Picogreen - measurement of ChIP Input DNA (reply: 9)
1984. PCR reagent autoclave - (reply: 2)
1985. weird site directed mutagenesis products - (reply: 3)
1986. extraction of viral rna from plant tissue - (reply: 1)
1987. ampicillin for LB agar - (reply: 2)
1988. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
1989. PCR not working - overhangs too long? (reply: 3)
1990. What melting temperature to use - (reply: 1)
1991. PFGE Current? - (reply: 1)
1992. Plasmid frameshift problem after clonning - (reply: 2)
1993. Nde1-BamH1 makes me sad - high background despite DIFFERENT methods (reply: 34)
1994. TA cloning necessary? - (reply: 2)
1995. Is DNA an acid because of phosphate group - (reply: 2)
1996. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
1997. colony formation assay - how many cells to seed (reply: 2)
1998. Conversion of primer unit - ug/ul to uM (reply: 4)
1999. mutagenesis by PCR or just use a kit - (reply: 5)
2000. Drying RNA in Speedvac - (reply: 1)
2001. cDNA for qPCR - Help with basics please (reply: 6)
2002. plasmid extraction kit solution composition - wash buffer (reply: 2)
2003. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
2004. Primer Trouble Shooting - (reply: 5)
2005. Proven Primers Stopped working & now leave smear! - (reply: 2)
2006. Expired chemicals - 0.5M EDTA, can be used again after autoclaved? - (reply: 4)
2007. tri reagent not compatible with plastic? - (reply: 1)
2008. DNA Extraction - (reply: 2)
2009. ligation problem - (reply: 6)
2010. TRIzol v. TRI reagent - (reply: 2)