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Top : New Forum Archives (2009-): : Molecular-Biology
1981. Plasmid frameshift problem after clonning - (reply: 2)
1982. Nde1-BamH1 makes me sad - high background despite DIFFERENT methods (reply: 34)
1983. TA cloning necessary? - (reply: 2)
1984. Is DNA an acid because of phosphate group - (reply: 2)
1985. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
1986. colony formation assay - how many cells to seed (reply: 2)
1987. Conversion of primer unit - ug/ul to uM (reply: 4)
1988. mutagenesis by PCR or just use a kit - (reply: 5)
1989. Drying RNA in Speedvac - (reply: 1)
1990. cDNA for qPCR - Help with basics please (reply: 6)
1991. plasmid extraction kit solution composition - wash buffer (reply: 2)
1992. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
1993. Primer Trouble Shooting - (reply: 5)
1994. Proven Primers Stopped working & now leave smear! - (reply: 2)
1995. Expired chemicals - 0.5M EDTA, can be used again after autoclaved? - (reply: 4)
1996. tri reagent not compatible with plastic? - (reply: 1)
1997. DNA Extraction - (reply: 2)
1998. ligation problem - (reply: 6)
1999. TRIzol v. TRI reagent - (reply: 2)
2000. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
2001. Isolation of DNA from Scat - (reply: 2)
2002. How long will cellular resources last - (reply: 1)
2003. PFGE conditions - (reply: 2)
2004. genomic DNA isolation - (reply: 4)
2005. Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
2006. How to find gene homologues? - (reply: 1)
2007. IHC vs qPCR - is this possible (reply: 1)
2008. homolog detection using Southern - (reply: 1)
2009. restriction - (reply: 2)
2010. T7 transcription - (reply: 1)