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Top : New Forum Archives (2009-): : Molecular-Biology
1981. mutagenesis by PCR or just use a kit - (reply: 5)
1982. Drying RNA in Speedvac - (reply: 1)
1983. cDNA for qPCR - Help with basics please (reply: 6)
1984. plasmid extraction kit solution composition - wash buffer (reply: 2)
1985. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
1986. Primer Trouble Shooting - (reply: 5)
1987. Proven Primers Stopped working & now leave smear! - (reply: 2)
1988. Expired chemicals - 0.5M EDTA, can be used again after autoclaved? - (reply: 4)
1989. tri reagent not compatible with plastic? - (reply: 1)
1990. DNA Extraction - (reply: 2)
1991. ligation problem - (reply: 6)
1992. TRIzol v. TRI reagent - (reply: 2)
1993. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
1994. Isolation of DNA from Scat - (reply: 2)
1995. How long will cellular resources last - (reply: 1)
1996. PFGE conditions - (reply: 2)
1997. genomic DNA isolation - (reply: 4)
1998. Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
1999. How to find gene homologues? - (reply: 1)
2000. IHC vs qPCR - is this possible (reply: 1)
2001. homolog detection using Southern - (reply: 1)
2002. restriction - (reply: 2)
2003. T7 transcription - (reply: 1)
2004. measuring apoptosis - reduced cell proliferation due to apoptosis (reply: 1)
2005. 10X MOPS buffer for RNA gels - It is yellow! (reply: 3)
2006. RNA isolation with peqGold TriFast - (reply: 2)
2007. Pipette tips for gel electrophoresis? - (reply: 4)
2008. Problem with a Double digestion - ClaI/Esp3I digestion does not want to work! (reply: 6)
2009. addition of BclI restriction site to PCR primer - (reply: 6)
2010. storing RNA at -20C? - (reply: 4)