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Top : New Forum Archives (2009-): : Molecular-Biology
1981. Problem with a Double digestion - ClaI/Esp3I digestion does not want to work! (reply: 6)
1982. addition of BclI restriction site to PCR primer - (reply: 6)
1983. storing RNA at -20C? - (reply: 4)
1984. questions on gene therapy in brain - (reply: 1)
1985. RACE kits - opinions - What brand do you use to get RACE ready cDNA? (reply: 2)
1986. How to do PCR to detect mRNA without RT? - (reply: 2)
1987. Electroporation Arcing - I'm a newbie, any pointers welcome (reply: 3)
1988. quality of a paper - (reply: 3)
1989. Gel Electrophoresis band problems - (reply: 3)
1990. first strand cDNA synthesis not working - cannot PCR out cDNAs of interest from first strand cDNA (reply: 2)
1991. How to remove copy of redundant cDNA in a mixture - RT results in a cDNA library with high copy number of few genes (reply: 3)
1992. Northern blot with mRNA - (reply: 2)
1993. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
1994. is it necessary to use RNAlater for tissue samples? - (reply: 1)
1995. RNA gel to test DIG labeled RNA probe - (reply: 1)
1996. EPI300 CopyControl Solution - Does anyone know the ingredients? (reply: 2)
1997. Strange Sequencing results - (reply: 4)
1998. Inserting a reporter into the genome - (reply: 1)
1999. Sequences Needed, mYFP, mCFP, tdTomato - We are missing these sequences :(( (reply: 7)
2000. wacky RNA gel - (reply: 1)
2001. Methylation analysis in vitro - (reply: 3)
2002. LacZ PCR genotyping -ve control contamination - (reply: 3)
2003. Northern blot - (reply: 1)
2004. Isolation of protein crosslinked to plasmid - (reply: 3)
2005. Inseparable insert in gel purification of the vector - (reply: 3)
2006. Reversible block, 3' OH? - (reply: 2)
2007. pcDNA3-FLAG-p53 Sequence - (reply: 6)
2008. cloning - how to clone a gene having stop codons (reply: 4)
2009. Unhappy bands - (reply: 3)
2010. dam methylated DNA in dam - straights - (reply: 1)