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Top : New Forum Archives (2009-): : Molecular-Biology
1981. have problems with electroporation transformation of PCP20 into BL21 de3 - (reply: 2)
1982. subcloning problem - inframe fusion (reply: 1)
1983. SSCP - (reply: 2)
1984. OD reading - (reply: 1)
1985. E coli transformation - (reply: 8)
1986. RNAsin overdose! - (reply: 1)
1987. Gel loading dye : sample ratio?? - have to follow strictly? (reply: 6)
1988. how to intentionally alter gene sequence - (reply: 1)
1989. molecular biology - (reply: 2)
1990. How to remove diphosphate from in vitro transcribed RNA? - (reply: 1)
1991. Experience with YM-30 microcon columns from Millipore - (reply: 2)
1992. DNA stains - pronunciation question (reply: 3)
1993. Floating white pellet in miniprep - (reply: 2)
1994. Gel electrophoresis problem - (reply: 7)
1995. Making competent cell from freeze-thawed stock - (reply: 2)
1996. Fluorescent measurements-AMCA - (reply: 4)
1997. northern prob - (reply: 4)
1998. dna smear in electrophoresis - (reply: 6)
1999. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
2000. Reverse-transcription of UV-damaged RNA - (reply: 3)
2001. How could I express two genes in opposite orientation - (reply: 2)
2002. shRNA oligo annealing problem - (reply: 5)
2003. Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)
2004. GFP insert - trying to insert GFP in a vector (reply: 1)
2005. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
2006. miRNA northern - (reply: 1)
2007. Double digestion and cloning - (reply: 2)
2008. Plasmid problem - From PCR product (reply: 7)
2009. cloning of long fragment - (reply: 1)
2010. how to find out the level of expression among different transcripts of the same - (reply: 1)