Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1981. ccdB survival E. coli transformation - (reply: 2)
1982. how to get higher RNA purity? - (reply: 3)
1983. Snap freezing worm samples for RNA prep - is it essential? (reply: 1)
1984. Adding A overhangs - primer design implications? - (reply: 1)
1985. I can't resolve 2 proteins (LC3-I and II) in Tricine gels - (reply: 5)
1986. Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
1987. Fungal DNA Isolation - Fungal DNA isolation without liquid nitrogen (reply: 1)
1988. Translational Efficiancy - (reply: 1)
1989. Electroporation strange problem - (reply: 1)
1990. reverse transcriptase and cDNA - (reply: 1)
1991. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
1992. stable cell line - (reply: 2)
1993. Site-directed mutagenesis (repeat oligo insert) - Strange mutagenesis reults! (reply: 10)
1994. Low RNA yield after extraction by RNA blood mini kit. - (reply: 2)
1995. Restriction Enzymes - Enzymes from different companies work together? (reply: 2)
1996. Plant RNA Isolation Aid - (reply: 3)
1997. best plasmid purification kit for transfection - (reply: 1)
1998. insert sequence in clone - (reply: 6)
1999. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
2000. how long is carb stable in liquid broth at 37C - I need longer incubation times of bacteria because of the transformant (reply: 1)
2001. Blotting technique principle - (reply: 1)
2002. any one tried in-vitro expression system ? - Roche is the best ? (reply: 1)
2003. Taq at 2-8 - (reply: 4)
2004. cloning in pET 30 B - (reply: 1)
2005. molcecular biology protocols - name of the famous series of books (reply: 2)
2006. cell adhesion assay - collagen (reply: 1)
2007. freezing during RNA isolation step - (reply: 1)
2008. Restriction enzyme buffer running out ? - (reply: 1)
2009. Low 260/230 ratio in my RNA - (reply: 2)
2010. Help for some general DNA Extraction Questions - (reply: 5)