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Top : New Forum Archives (2009-): : Molecular-Biology
421. What is the necessary time for lipofectamin to make complex with pre-miRNA oligo - (reply: 1)
422. what is the ORFeome clone? - (reply: 2)
423. ligation with one ristrection site/enzyme - (reply: 3)
424. No amplification during RT-PCR - (reply: 4)
425. rescue cloning of a transposon mutant - (reply: 2)
426. Dephosphorylation - (reply: 7)
427. study of receptors in cell lines - (reply: 1)
428. Mouse genotyping issues - (reply: 1)
429. In-situ hybridization - (reply: 1)
430. His tag introduction in to gene and primer design - (reply: 2)
431. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
432. Principle of silica membrane binding of nucleic acid - (reply: 6)
433. genogroup and genotype - (reply: 2)
434. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
435. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
436. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
437. Beta alanine alternative? - (reply: 2)
438. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
439. lncRNA full-sequence or partial sequence - (reply: 2)
440. Cloning at 6 kb gene - (reply: 3)
441. Trouble with DNA purity (A260/A280) - (reply: 3)
442. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
443. Luciferase assay - extreme variation in RLU - (reply: 1)
444. site-directed mutagenesis - (reply: 20)
445. qPCR detection - (reply: 2)
446. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
447. TCA precipitation - (reply: 1)
448. SOC vs TB in transformations - (reply: 1)
449. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
450. Single RE site cloning - (reply: 3)