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Top : New Forum Archives (2009-): : Molecular-Biology
421. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
422. Principle of silica membrane binding of nucleic acid - (reply: 6)
423. genogroup and genotype - (reply: 2)
424. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
425. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
426. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
427. Beta alanine alternative? - (reply: 2)
428. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
429. lncRNA full-sequence or partial sequence - (reply: 2)
430. Cloning at 6 kb gene - (reply: 3)
431. Trouble with DNA purity (A260/A280) - (reply: 3)
432. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
433. Luciferase assay - extreme variation in RLU - (reply: 1)
434. site-directed mutagenesis - (reply: 20)
435. qPCR detection - (reply: 2)
436. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
437. TCA precipitation - (reply: 1)
438. SOC vs TB in transformations - (reply: 1)
439. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
440. Single RE site cloning - (reply: 3)
441. PCR product running on agarose gel - (reply: 32)
442. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
443. PCR amplification with high fidelity enzyme - (reply: 1)
444. Transformation problem - (reply: 5)
445. finding gene sequence to delete - (reply: 4)
446. Blue fluorescent color of agarose gel in UV - (reply: 7)
447. heterologous gene integration into e.coli genome - (reply: 1)
448. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
449. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
450. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)