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421. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
422. PCR amplification with high fidelity enzyme - (reply: 1)
423. Transformation problem - (reply: 5)
424. finding gene sequence to delete - (reply: 4)
425. Blue fluorescent color of agarose gel in UV - (reply: 7)
426. heterologous gene integration into e.coli genome - (reply: 1)
427. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
428. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
429. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
430. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
431. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
432. Continuous growth of E. coli on AMP LB plate - (reply: 2)
433. Plasmid compatibly groups - (reply: 5)
434. too much DNA on agarose gel - (reply: 9)
435. What are the "mammalian vectors N1 and C1"? - (reply: 1)
436. Life before BLAST etc? Help needed... - (reply: 1)
437. How base pairs upstream of gene to include for the promoter? - (reply: 3)
438. Neomycin resistance sequence - (reply: 1)
439. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
440. polyacrylamide RNA gel - (reply: 4)
441. Making a standard curve from bacterial suspensions - (reply: 1)
442. Is my RNA degraded? - (reply: 7)
443. Phosphotyrosine western blotting, HELP! - (reply: 1)
444. Bacteriophage genome extraction - (reply: 1)
445. Fast, cheap and dependable DNA extraction protocol for students? - (reply: 3)
446. western blot troubleshooting - (reply: 5)
447. Sanger Sequencing RNAse Interference? - (reply: 1)
448. isolating DNA from cells in suspension - (reply: 5)
449. survival strategy of bacteria - (reply: 4)
450. can restriction enzymes be stored at -70°C? - (reply: 3)
422. PCR amplification with high fidelity enzyme - (reply: 1)
423. Transformation problem - (reply: 5)
424. finding gene sequence to delete - (reply: 4)
425. Blue fluorescent color of agarose gel in UV - (reply: 7)
426. heterologous gene integration into e.coli genome - (reply: 1)
427. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
428. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
429. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
430. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
431. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
432. Continuous growth of E. coli on AMP LB plate - (reply: 2)
433. Plasmid compatibly groups - (reply: 5)
434. too much DNA on agarose gel - (reply: 9)
435. What are the "mammalian vectors N1 and C1"? - (reply: 1)
436. Life before BLAST etc? Help needed... - (reply: 1)
437. How base pairs upstream of gene to include for the promoter? - (reply: 3)
438. Neomycin resistance sequence - (reply: 1)
439. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
440. polyacrylamide RNA gel - (reply: 4)
441. Making a standard curve from bacterial suspensions - (reply: 1)
442. Is my RNA degraded? - (reply: 7)
443. Phosphotyrosine western blotting, HELP! - (reply: 1)
444. Bacteriophage genome extraction - (reply: 1)
445. Fast, cheap and dependable DNA extraction protocol for students? - (reply: 3)
446. western blot troubleshooting - (reply: 5)
447. Sanger Sequencing RNAse Interference? - (reply: 1)
448. isolating DNA from cells in suspension - (reply: 5)
449. survival strategy of bacteria - (reply: 4)
450. can restriction enzymes be stored at -70°C? - (reply: 3)