Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
421. Low yield for small RNA isolation - (reply: 1)
422. Primers to amplify Kan operon from pet28 - (reply: 2)
423. Questions about proper technical approach - (reply: 6)
424. RNA extraction: low RCF by mistake - (reply: 1)
425. Avoid SDS in thermal lysis: whole blood? - (reply: 1)
426. What is the role of SV40 sequence in "5'UTR - GFP - SV40"? - (reply: 1)
427. Transfection of a non human gene into human cell line - (reply: 1)
428. DNA extraction from non-viable PBMCs - (reply: 2)
429. Visualizing RNA on blot - (reply: 1)
430. Can "3' - biotinylated oligo" be ligased with "5' phosphoryl - (reply: 3)
431. expression plasmids for tRNA - (reply: 5)
432. a promoter question, two promoter cassetts-related expression - (reply: 2)
433. Protein and RNA interaction - (reply: 1)
434. Transcription factors involved in mouse lung transfection - EMSA, pull-down- how - (reply: 3)
435. how can I remove the pigments during DNA extraction? - (reply: 1)
436. how to remove brown pigment in Sargassum DNA isolation? - (reply: 4)
437. difference between CDNA & CDS - (reply: 1)
438. improper recombination in transgenic mice - (reply: 1)
439. contamination of human cell lines with yeast - (reply: 3)
440. Barcoding PCR products - (reply: 18)
441. Re-culturing empty plasmid - (reply: 1)
442. IMAC nickel solution - (reply: 1)
443. Large scale Viral RNA purification Kit - (reply: 3)
444. UTR sequences after IRES - (reply: 1)
445. Storing Bacterial Culture - (reply: 1)
446. MTT: for 5 days - (reply: 1)
447. T7 promoter ending; to add or not to add GGG - (reply: 2)
448. Cytoplasmic mRNA isolation protocol - (reply: 1)
449. BSA in PCR reaction - (reply: 1)
450. Adding T7 promoter sequence to vector - (reply: 2)