421. Midi prep extraction problem - (reply: 2)
422. dna left on thermocycler - (reply: 2)
423. Cleaning probes with 1% DEPC water. - (reply: 3)
424. a smeared Gen-DNA template ---> smear and less yield on pcr ? - (reply: 1)
425. PCR amplification of large template - (reply: 1)
426. Bacteria grew on plates but not in liquid culture - (reply: 1)
427. Doubt: genomic dna library construction - (reply: 3)
428. DH5alpha transformation protocol - (reply: 1)
429. Strange Negative Contamination Issue. - (reply: 2)
430. smear in gel electrophoresis after PCR - (reply: 2)
431. How to quantify MS2 virus-like particles? - (reply: 1)
432. Drugs that cause DNA double strand breaks - (reply: 1)
433. Blue middle tint in white colonies for Blue/White screening - (reply: 2)
434. SBI Minicircle DNA Kit -- No colonies -- ligation / transformation problem - (reply: 8)
435. No DNA after gel extraction - (reply: 9)
436. Cloning synthesised genes - (reply: 1)
437. DNA isolation from nuclei - (reply: 5)
438. DNA in 1water:0,1TE at 37 degrees - (reply: 1)
439. Why do people typically use AP instead of HRP for Southerns? - (reply: 2)
440. Difference between streptavidin-bound nucleic acid probe and probe covalently at - (reply: 5)
441. Low Transformation Efficiency with a Specific Gene - (reply: 4)
442. Plasmid DNA contamination - (reply: 5)
443. Problems with plasmid DNA extraction - (reply: 3)
444. Newbie to smaI blunt end cloning! - (reply: 1)
445. What part of the promoter to clone - (reply: 2)
446. PCR RFLP is PCR product purification necessary? - (reply: 3)
447. Biotinylated RNA on Nylon Membrane - (reply: 2)
448. conditionally active plasmids - (reply: 1)
449. UV crosslinkers, changing UV bulbs? - (reply: 1)
450. percentage DNA fragmentation - (reply: 1)

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