61. Question about CaCl2 and CaCl2 - (reply: 5)
62. Preservation of RNA/cDNA samples - (reply: 1)
63. Cycloheximide concentration - (reply: 2)
64. Cell cycle - (reply: 2)
65. Design of <=40bp primers adding restriction sites and also Gibson Assembly o - (reply: 1)
66. Very low yields for >10kb fragment gel extractions - (reply: 2)
67. DNA extraction with phenol-chloroform - DNA stuck in phenol layer? - (reply: 1)
68. Cloning of big inserts - (reply: 2)
69. Primer design HELP! - (reply: 4)
70. wnt/beta catenin signalling - (reply: 1)
71. Using RNA minikit for DNA extraction - (reply: 4)
72. Getting terrible grainy background in immunofluorescence experiment - (reply: 5)
73. Purification of linearized AT-rich DNA for transfection - (reply: 4)
74. DNA decontamination chemistry - (reply: 1)
75. Why is the smearing becoming different in the polyacrylamide gel than the agaros - (reply: 2)
76. Site directed mutagenesis stopped working. - (reply: 6)
77. transfection - (reply: 2)
78. multiple melting curves - (reply: 1)
79. cell invasion assay - (reply: 1)
80. a half less of plasmid copy number in the solution resulting from ddPCR than qPC - (reply: 1)
81. Need to run gel after miniprep before digest? - (reply: 1)
82. Good diluents for RNA - (reply: 1)
83. solution preparation - (reply: 1)
84. concentration of starting material - (reply: 1)
85. Qiagen ATL, AL, AW1, AW2 recipes? - (reply: 1)
86. site directed mutagenesis - (reply: 1)
87. DNA concentration - (reply: 1)
88. what‘s phage display principle - (reply: 1)
89. pGEM T Easy subcloning - (reply: 1)
90. autoantibody - (reply: 1)

© 1999-2013 Protocol Online, All rights reserved.