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61. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
62. What is the necessary time for lipofectamin to make complex with pre-miRNA oligo - (reply: 1)
63. what is the ORFeome clone? - (reply: 2)
64. ligation with one ristrection site/enzyme - (reply: 3)
65. No amplification during RT-PCR - (reply: 4)
66. rescue cloning of a transposon mutant - (reply: 2)
67. Dephosphorylation - (reply: 7)
68. study of receptors in cell lines - (reply: 1)
69. Mouse genotyping issues - (reply: 1)
70. In-situ hybridization - (reply: 1)
71. His tag introduction in to gene and primer design - (reply: 2)
72. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
73. Principle of silica membrane binding of nucleic acid - (reply: 6)
74. genogroup and genotype - (reply: 2)
75. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
76. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
77. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
78. Beta alanine alternative? - (reply: 2)
79. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
80. lncRNA full-sequence or partial sequence - (reply: 2)
81. Cloning at 6 kb gene - (reply: 3)
82. Trouble with DNA purity (A260/A280) - (reply: 3)
83. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
84. Luciferase assay - extreme variation in RLU - (reply: 1)
85. site-directed mutagenesis - (reply: 20)
86. qPCR detection - (reply: 2)
87. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
88. TCA precipitation - (reply: 1)
89. SOC vs TB in transformations - (reply: 1)
90. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
62. What is the necessary time for lipofectamin to make complex with pre-miRNA oligo - (reply: 1)
63. what is the ORFeome clone? - (reply: 2)
64. ligation with one ristrection site/enzyme - (reply: 3)
65. No amplification during RT-PCR - (reply: 4)
66. rescue cloning of a transposon mutant - (reply: 2)
67. Dephosphorylation - (reply: 7)
68. study of receptors in cell lines - (reply: 1)
69. Mouse genotyping issues - (reply: 1)
70. In-situ hybridization - (reply: 1)
71. His tag introduction in to gene and primer design - (reply: 2)
72. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
73. Principle of silica membrane binding of nucleic acid - (reply: 6)
74. genogroup and genotype - (reply: 2)
75. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
76. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
77. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
78. Beta alanine alternative? - (reply: 2)
79. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
80. lncRNA full-sequence or partial sequence - (reply: 2)
81. Cloning at 6 kb gene - (reply: 3)
82. Trouble with DNA purity (A260/A280) - (reply: 3)
83. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
84. Luciferase assay - extreme variation in RLU - (reply: 1)
85. site-directed mutagenesis - (reply: 20)
86. qPCR detection - (reply: 2)
87. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
88. TCA precipitation - (reply: 1)
89. SOC vs TB in transformations - (reply: 1)
90. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)