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331. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
332. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
333. Beta alanine alternative? - (reply: 2)
334. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
335. lncRNA full-sequence or partial sequence - (reply: 2)
336. Cloning at 6 kb gene - (reply: 3)
337. Trouble with DNA purity (A260/A280) - (reply: 3)
338. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
339. Luciferase assay - extreme variation in RLU - (reply: 1)
340. site-directed mutagenesis - (reply: 20)
341. qPCR detection - (reply: 2)
342. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
343. TCA precipitation - (reply: 1)
344. SOC vs TB in transformations - (reply: 1)
345. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
346. Single RE site cloning - (reply: 3)
347. PCR product running on agarose gel - (reply: 32)
348. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
349. PCR amplification with high fidelity enzyme - (reply: 1)
350. Transformation problem - (reply: 5)
351. finding gene sequence to delete - (reply: 4)
352. Blue fluorescent color of agarose gel in UV - (reply: 7)
353. heterologous gene integration into e.coli genome - (reply: 1)
354. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
355. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
356. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
357. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
358. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
359. Continuous growth of E. coli on AMP LB plate - (reply: 2)
360. Plasmid compatibly groups - (reply: 5)
332. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
333. Beta alanine alternative? - (reply: 2)
334. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
335. lncRNA full-sequence or partial sequence - (reply: 2)
336. Cloning at 6 kb gene - (reply: 3)
337. Trouble with DNA purity (A260/A280) - (reply: 3)
338. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
339. Luciferase assay - extreme variation in RLU - (reply: 1)
340. site-directed mutagenesis - (reply: 20)
341. qPCR detection - (reply: 2)
342. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
343. TCA precipitation - (reply: 1)
344. SOC vs TB in transformations - (reply: 1)
345. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
346. Single RE site cloning - (reply: 3)
347. PCR product running on agarose gel - (reply: 32)
348. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
349. PCR amplification with high fidelity enzyme - (reply: 1)
350. Transformation problem - (reply: 5)
351. finding gene sequence to delete - (reply: 4)
352. Blue fluorescent color of agarose gel in UV - (reply: 7)
353. heterologous gene integration into e.coli genome - (reply: 1)
354. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
355. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
356. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
357. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
358. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
359. Continuous growth of E. coli on AMP LB plate - (reply: 2)
360. Plasmid compatibly groups - (reply: 5)