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Top : New Forum Archives (2009-): : Molecular-Biology
331. pcr 10X buffer preparation - (reply: 2)
332. Sensitive, Consistent and Reproducible Method(s) for Extraction of gDNA from Low - (reply: 2)
333. Enhancer Analysis - (reply: 2)
334. how much template do i need for pcr? - (reply: 5)
335. 5'-azacytidine treatment - (reply: 1)
336. leaky promoter - (reply: 1)
337. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
338. SAP dephosphorylation - (reply: 1)
339. Determining in frame in modified vector - (reply: 1)
340. Deleting restriction enzyme sites - (reply: 1)
341. Creating primers to add restriction sites to vector backbone - (reply: 7)
342. cDNA test on 1% agarose gel - (reply: 3)
343. Plasmid isolation from gram positive bacteria - (reply: 2)
344. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
345. rsaI enzyme - (reply: 1)
346. How to check for the presence of plasmid? - (reply: 3)
347. Extracting total RNA from woody plant roots - (reply: 2)
348. Quantify total DNA in small cell lysate...? - (reply: 1)
349. Quantify DNA in gel electrophoresis bands - (reply: 7)
350. Protein extraction and isolectric focusing - (reply: 1)
351. getting strange data in Luciferase-renilla assay - (reply: 2)
352. Homozygote DNA but Heterozygote RNA? - (reply: 2)
353. sacB Counter-Selection to generate double cross-over deletion mutants - (reply: 8)
354. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
355. What is the necessary time for lipofectamin to make complex with pre-miRNA oligo - (reply: 1)
356. what is the ORFeome clone? - (reply: 2)
357. ligation with one ristrection site/enzyme - (reply: 3)
358. No amplification during RT-PCR - (reply: 4)
359. rescue cloning of a transposon mutant - (reply: 2)
360. Dephosphorylation - (reply: 7)