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331. Primers to amplify Kan operon from pet28 - (reply: 2)
332. Questions about proper technical approach - (reply: 6)
333. RNA extraction: low RCF by mistake - (reply: 1)
334. Avoid SDS in thermal lysis: whole blood? - (reply: 1)
335. What is the role of SV40 sequence in "5'UTR - GFP - SV40"? - (reply: 1)
336. Transfection of a non human gene into human cell line - (reply: 1)
337. DNA extraction from non-viable PBMCs - (reply: 2)
338. Visualizing RNA on blot - (reply: 1)
339. Can "3' - biotinylated oligo" be ligased with "5' phosphoryl - (reply: 3)
340. expression plasmids for tRNA - (reply: 5)
341. a promoter question, two promoter cassetts-related expression - (reply: 2)
342. Protein and RNA interaction - (reply: 1)
343. Transcription factors involved in mouse lung transfection - EMSA, pull-down- how - (reply: 3)
344. how can I remove the pigments during DNA extraction? - (reply: 1)
345. how to remove brown pigment in Sargassum DNA isolation? - (reply: 4)
346. difference between CDNA & CDS - (reply: 1)
347. improper recombination in transgenic mice - (reply: 1)
348. contamination of human cell lines with yeast - (reply: 3)
349. Barcoding PCR products - (reply: 16)
350. Re-culturing empty plasmid - (reply: 1)
351. IMAC nickel solution - (reply: 1)
352. Large scale Viral RNA purification Kit - (reply: 3)
353. UTR sequences after IRES - (reply: 1)
354. Storing Bacterial Culture - (reply: 1)
355. MTT: for 5 days - (reply: 1)
356. T7 promoter ending; to add or not to add GGG - (reply: 2)
357. Cytoplasmic mRNA isolation protocol - (reply: 1)
358. BSA in PCR reaction - (reply: 1)
359. Adding T7 promoter sequence to vector - (reply: 2)
360. Can CMV promoter start transcription in prokaryote cells - (reply: 1)
332. Questions about proper technical approach - (reply: 6)
333. RNA extraction: low RCF by mistake - (reply: 1)
334. Avoid SDS in thermal lysis: whole blood? - (reply: 1)
335. What is the role of SV40 sequence in "5'UTR - GFP - SV40"? - (reply: 1)
336. Transfection of a non human gene into human cell line - (reply: 1)
337. DNA extraction from non-viable PBMCs - (reply: 2)
338. Visualizing RNA on blot - (reply: 1)
339. Can "3' - biotinylated oligo" be ligased with "5' phosphoryl - (reply: 3)
340. expression plasmids for tRNA - (reply: 5)
341. a promoter question, two promoter cassetts-related expression - (reply: 2)
342. Protein and RNA interaction - (reply: 1)
343. Transcription factors involved in mouse lung transfection - EMSA, pull-down- how - (reply: 3)
344. how can I remove the pigments during DNA extraction? - (reply: 1)
345. how to remove brown pigment in Sargassum DNA isolation? - (reply: 4)
346. difference between CDNA & CDS - (reply: 1)
347. improper recombination in transgenic mice - (reply: 1)
348. contamination of human cell lines with yeast - (reply: 3)
349. Barcoding PCR products - (reply: 16)
350. Re-culturing empty plasmid - (reply: 1)
351. IMAC nickel solution - (reply: 1)
352. Large scale Viral RNA purification Kit - (reply: 3)
353. UTR sequences after IRES - (reply: 1)
354. Storing Bacterial Culture - (reply: 1)
355. MTT: for 5 days - (reply: 1)
356. T7 promoter ending; to add or not to add GGG - (reply: 2)
357. Cytoplasmic mRNA isolation protocol - (reply: 1)
358. BSA in PCR reaction - (reply: 1)
359. Adding T7 promoter sequence to vector - (reply: 2)
360. Can CMV promoter start transcription in prokaryote cells - (reply: 1)