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1411. QCMD samples - (reply: 5)
1412. competent cells - OD600 reading (reply: 6)
1413. Creating overhangs with PCR - (reply: 4)
1414. RNA: Is this degratation or something else? - (reply: 1)
1415. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1416. Is there any software that can be sued to design degenerate primers - (reply: 1)
1417. Primer Decontamination - (reply: 2)
1418. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1419. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1420. How long is "overnight"? - (reply: 3)
1421. Trizol stability - (reply: 1)
1422. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1423. Tube Openers - (reply: 3)
1424. taq man probes non specific? - (reply: 1)
1425. Help! low efficiency of transfection - (reply: 2)
1426. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1427. Cloning before sequencing- is it necessary??? - (reply: 13)
1428. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1429. Guanadine HCl for plasmid miniprep - (reply: 3)
1430. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1431. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1432. 3īRACE problem - only smear - (reply: 4)
1433. salt for DNA precipitation - DNA purification (reply: 3)
1434. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1435. Not getting plasmid from miniprep - (reply: 7)
1436. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1437. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1438. Is this consider smearing? - (reply: 2)
1439. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1440. grow in broth but not on agar... - after transformation (reply: 2)