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Top : New Forum Archives (2009-): : Molecular-Biology
2221. Mutation during transformation/maxiprep - (reply: 1)
2222. beta actin - loading control (reply: 1)
2223. Tranformation cell harvesting - (reply: 5)
2224. Sequencing - identifying backbone and deciphering insert information - (reply: 8)
2225. dna quantification and quality assesement - abs 260nm / abs 280 nm <0.5 (reply: 2)
2226. Best plasmid miniprep? - (reply: 13)
2227. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
2228. Volume of DNA to add to PCR Master Mix? - (reply: 1)
2229. PCR components storage - (reply: 3)
2230. problem in chemiluminiscent EMSA PAGE running - (reply: 2)
2231. Alkaline phosphatase - (reply: 5)
2232. resolving a 100bp difference at around 1.2kb (AGE) - (reply: 2)
2233. RNA, mRNA, sequencing... HELP - (reply: 2)
2234. No PCR bands with some templates - (reply: 5)
2235. RNA Purification - After DNase (reply: 3)
2236. How To Fix NaOH/Tris DNA Digest Error? - (reply: 1)
2237. Taq Dilution - (reply: 8)
2238. Homogenation of Mouse Spinal Cord Tissue for RNA Extraction - Most effective way to do it? (reply: 3)
2239. PCR product purification - (reply: 3)
2240. EMSA - Band doesn't shift with poly dI-dC - (reply: 2)
2241. Temp Gradient PCR - (reply: 2)
2242. BamHI and HindIII double digest - driving me mad... (reply: 1)
2243. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
2244. rna isolation - (reply: 1)
2245. DNA extraction with expired kit? - (reply: 1)
2246. Transcription of double blunt end Clone - (reply: 2)
2247. PCR with genomic dna - (reply: 9)
2248. mutagenesis - (reply: 1)
2249. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
2250. PCR with long primers - (reply: 3)