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Top : New Forum Archives (2009-): : Molecular-Biology
2221. PCR with long primers - (reply: 3)
2222. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
2223. Gateway cloning - (reply: 8)
2224. RNA Isolation - RNA isolation from human cell lines (reply: 2)
2225. Which red fluorescence is better? - mCherry of dsRed (reply: 2)
2226. Finding corresponding amino acid in DNA sequence - (reply: 1)
2227. Buffer for 5 Kb DNA fragments - (reply: 2)
2228. Pooling doesn't work - make up rna amount of 10ug (reply: 3)
2229. Western Blot for Bacteria - Loading Control (reply: 1)
2230. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
2231. Southern blotting - copy number determination (reply: 1)
2232. RNase: RNA ratio - making dsRNA (reply: 1)
2233. I hate my Klenow! - (reply: 9)
2234. Tris Base Buffer is the same that Trizma??No I think.. - Buffers:Differences between Tris Base and Trizma.. (reply: 3)
2235. Sequencing doubts - (reply: 3)
2236. Help with Northern Blotting - (reply: 4)
2237. ELISA - how can i get Mab for NS1? (reply: 1)
2238. 0.5% crystal violet - how to make (reply: 2)
2239. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
2240. cDNA 260/280 ratio - (reply: 3)
2241. Amino acid substitution a problem? - Phenylalanine to leucine (reply: 3)
2242. What would happen? sequencing question - (reply: 2)
2243. Chemical transfection with Calcium Chloride - anyone used this method and made the 2X HBS? (reply: 6)
2244. About reporting a new splice variant in NCBI - (reply: 1)
2245. foci formation assay - crystal violet (reply: 1)
2246. In situ hybridization - zebrafish (reply: 1)
2247. transient transfection - (reply: 2)
2248. Can I still use 4-year-old Dig-labeled Marker ? - (reply: 4)
2249. Dot Blot Assay - (reply: 2)
2250. plasmid sequence identification problem - (reply: 2)