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Top : New Forum Archives (2009-): : Molecular-Biology
2221. The protocol for double digestion with BamHI and SacI - (reply: 15)
2222. stable transfection - V5-tag antibody (reply: 1)
2223. GAPDH Tissue Distribution - (reply: 1)
2224. Simple ethanol precipitation was a disaster and have no idea why! - (reply: 7)
2225. Oligo concentration determination - Is this correct? (reply: 2)
2226. Keeping check of an In-situ hybridisation experiment in general and making good - (reply: 4)
2227. Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene - (reply: 2)
2228. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
2229. RNA extraction 260/230 ratio - (reply: 6)
2230. cDNA evaluation and quantification - (reply: 5)
2231. Experimental Design Problem for RNA FISH - (reply: 1)
2232. cytoplasmic/nuclear fraction - (reply: 1)
2233. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
2234. Nonradiactive labelling of probes - (reply: 1)
2235. Accidently heated up the wrong DNA. Damage? - (reply: 2)
2236. genomic DNA quality for genome walking - (reply: 2)
2237. Old NaOH no good? - (reply: 4)
2238. Theoretical question about retroviruses - (reply: 1)
2239. Blue juice - Bromophenol blue or Bromophenol blue sodium salt (reply: 2)
2240. Oligo storage - 4C ?? (reply: 3)
2241. Precipitation appears after adding sodium acetate but before adding cold ethanol - (reply: 8)
2242. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
2243. Site directed mutagenesis - (reply: 2)
2244. Two bands for p53 on Western Blot - (reply: 7)
2245. Mutation during transformation/maxiprep - (reply: 1)
2246. beta actin - loading control (reply: 1)
2247. Tranformation cell harvesting - (reply: 5)
2248. Sequencing - identifying backbone and deciphering insert information - (reply: 8)
2249. dna quantification and quality assesement - abs 260nm / abs 280 nm <0.5 (reply: 2)
2250. Best plasmid miniprep? - (reply: 13)