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Top : New Forum Archives (2009-): : Molecular-Biology
1711. help with transformation efficiency calculation! - (reply: 1)
1712. Precipitation in DNAse I incubation buffer - (reply: 1)
1713. Taq enzyme works but other enzyme didn't work - (reply: 5)
1714. DNA purification - DNA purification dissolved in water (reply: 3)
1715. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
1716. PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
1717. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
1718. Double digestion using EcoRI and HindIII with pHAT-CAT - (reply: 4)
1719. dh5 alpha competent cells? - (reply: 2)
1720. Hypoxia - downregulated HIF-1A - why me??? (reply: 2)
1721. Bioanalyzer not recognising the chip - chip misaligned/manufacturing problem? - (reply: 4)
1722. transient transfection - (reply: 1)
1723. Bad sequence - (reply: 5)
1724. Southern Blot troubleshooting - Please help (reply: 3)
1725. Removing sequencing migration artefacts - (reply: 1)
1726. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
1727. Probes design for southern-blot - need more information for probes design for southern-blot (reply: 3)
1728. finding promoter - (reply: 1)
1729. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
1730. Normalizing RNA concentrations prior to cDNA production - qPCR and making cDNA (reply: 1)
1731. precipitation of secreted protein from growth media - (reply: 1)
1732. EMSA gel problem - (reply: 2)
1733. cloning - (reply: 6)
1734. Generating stable cell line - (reply: 2)
1735. Can't get clean RNA prep with TRIzol - (reply: 2)
1736. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
1737. vectors maps - (reply: 2)
1738. Fusion of reporter genes - (reply: 2)
1739. reuse electroporation cuvette? - (reply: 4)
1740. PCR Master mix - (reply: 1)