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1621. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
1622. how long is carb stable in liquid broth at 37C - I need longer incubation times of bacteria because of the transformant (reply: 1)
1623. Blotting technique principle - (reply: 1)
1624. any one tried in-vitro expression system ? - Roche is the best ? (reply: 1)
1625. Taq at 2-8 - (reply: 4)
1626. cloning in pET 30 B - (reply: 1)
1627. molcecular biology protocols - name of the famous series of books (reply: 2)
1628. cell adhesion assay - collagen (reply: 1)
1629. freezing during RNA isolation step - (reply: 1)
1630. Restriction enzyme buffer running out ? - (reply: 1)
1631. Low 260/230 ratio in my RNA - (reply: 2)
1632. Help for some general DNA Extraction Questions - (reply: 5)
1633. Replacement of Promoter - To replace araBAD promoter to T7 promoter (reply: 6)
1634. Gel loading dye recipe - trying to DIY Qiagen gel loading dye (reply: 4)
1635. how to check the quality of DNA? - (reply: 4)
1636. topoisomerase problem - (reply: 1)
1637. functional analysis of intron mutations - (reply: 7)
1638. 20mer Oligonucleotide running in the wrong direction - (reply: 1)
1639. Gateway on BAC - Perform LR reaction on long constructs (reply: 2)
1640. Can Morpholino oligo be delivered in oocytes by lipofectin - (reply: 2)
1641. Separating PCR product on agarose gel with similar sizes - (reply: 5)
1642. Vector map for pMH1 - Can't find it anywhere! (reply: 2)
1643. DNA precipitation - (reply: 3)
1644. No RNA after Trizol extraction - Lamba max: 230 !!! (reply: 8)
1645. yeast two hybrid - (reply: 1)
1646. MTT - cell prolferation vs apoptosis (reply: 2)
1647. Homogenising tissue, what do you rinse with? - (reply: 4)
1648. Extracting precious tissue from too large of volumes of RNAlater - stored tissue in too large of volume of RNAlater, can't recover (reply: 5)
1649. mRNA Bioanalyzer - (reply: 1)
1650. Doubt: Trizol protocol - about certain steps (reply: 9)
1622. how long is carb stable in liquid broth at 37C - I need longer incubation times of bacteria because of the transformant (reply: 1)
1623. Blotting technique principle - (reply: 1)
1624. any one tried in-vitro expression system ? - Roche is the best ? (reply: 1)
1625. Taq at 2-8 - (reply: 4)
1626. cloning in pET 30 B - (reply: 1)
1627. molcecular biology protocols - name of the famous series of books (reply: 2)
1628. cell adhesion assay - collagen (reply: 1)
1629. freezing during RNA isolation step - (reply: 1)
1630. Restriction enzyme buffer running out ? - (reply: 1)
1631. Low 260/230 ratio in my RNA - (reply: 2)
1632. Help for some general DNA Extraction Questions - (reply: 5)
1633. Replacement of Promoter - To replace araBAD promoter to T7 promoter (reply: 6)
1634. Gel loading dye recipe - trying to DIY Qiagen gel loading dye (reply: 4)
1635. how to check the quality of DNA? - (reply: 4)
1636. topoisomerase problem - (reply: 1)
1637. functional analysis of intron mutations - (reply: 7)
1638. 20mer Oligonucleotide running in the wrong direction - (reply: 1)
1639. Gateway on BAC - Perform LR reaction on long constructs (reply: 2)
1640. Can Morpholino oligo be delivered in oocytes by lipofectin - (reply: 2)
1641. Separating PCR product on agarose gel with similar sizes - (reply: 5)
1642. Vector map for pMH1 - Can't find it anywhere! (reply: 2)
1643. DNA precipitation - (reply: 3)
1644. No RNA after Trizol extraction - Lamba max: 230 !!! (reply: 8)
1645. yeast two hybrid - (reply: 1)
1646. MTT - cell prolferation vs apoptosis (reply: 2)
1647. Homogenising tissue, what do you rinse with? - (reply: 4)
1648. Extracting precious tissue from too large of volumes of RNAlater - stored tissue in too large of volume of RNAlater, can't recover (reply: 5)
1649. mRNA Bioanalyzer - (reply: 1)
1650. Doubt: Trizol protocol - about certain steps (reply: 9)