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Top : New Forum Archives (2009-): : Molecular-Biology
1621. Not getting plasmid from miniprep - (reply: 7)
1622. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1623. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1624. Is this consider smearing? - (reply: 2)
1625. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1626. grow in broth but not on agar... - after transformation (reply: 2)
1627. How many colonies to screen (colony PCR)? - (reply: 4)
1628. Primer Design - aligning sequences for probe/primer design (reply: 2)
1629. Cloning problem - (reply: 2)
1630. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
1631. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1632. In situ hybridization enigma - (reply: 1)
1633. A cloning nightmare - (reply: 8)
1634. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1635. RNA fragmentation - (reply: 3)
1636. RNA extraction - how many cells do I need at least?? (reply: 4)
1637. RNA extraction protocol for small amounts of cells - (reply: 2)
1638. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
1639. DNA sample contaminated with lipids - (reply: 8)
1640. cloning problem - (reply: 5)
1641. colony PCR inconsistent - (reply: 4)
1642. Newbie to Sequencing Question - (reply: 1)
1643. Problem with gel extraction of DNA. - (reply: 1)
1644. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
1645. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
1646. genomic DNA isolation - (reply: 1)
1647. E. coli not growing in liquid cultures :( - (reply: 4)
1648. Tricine SDS-PAGE for plant mitochondria - Membrane proteomics (reply: 1)
1649. DNA product almost 1500 bp - (reply: 1)
1650. Slightly too high 260:280 and 260:230 ratios from gDNA extraction - Should I care? (reply: 2)