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1621. Transformation background problem - high background in negative control (reply: 9)
1622. Akrylamide Gels - (reply: 2)
1623. Does unautoclaved DEPC inhibit northern blotting? - (reply: 2)
1624. Miniprep and sequencing - (reply: 4)
1625. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
1626. Protein TAG for affinity purification - (reply: 1)
1627. Blunt-Cohesive ligation - Should I change the conditions? (reply: 2)
1628. GFP fused proteins - i'm going to cry... (reply: 3)
1629. what's the meaning? - HEAP (reply: 2)
1630. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
1631. Making gels in advance - (reply: 2)
1632. Phenol-chloroform problem - (reply: 1)
1633. Cloning <100 bp fragments into a plasmid - (reply: 7)
1634. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
1635. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
1636. Amount of DNase to use (Qiagen) - (reply: 7)
1637. COMPLETE RNA degradation - (reply: 18)
1638. HpaI blunt end cloning - (reply: 1)
1639. undesired products in multiplex PCR - (reply: 3)
1640. Generate Supercoil Plasmid - it just a thought... (reply: 5)
1641. help with protein localization study using GFP - (reply: 5)
1642. Does a viral gene have introns? - (reply: 4)
1643. Selecting Lethal Mutants? - (reply: 1)
1644. RNA extraction from stained cells - (reply: 2)
1645. agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
1646. No band after digestion - (reply: 4)
1647. Problem with ligation - Insert into the "wrong" vector (reply: 11)
1648. finding promoter - (reply: 1)
1649. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
1650. Cloning in pCR2.1 plasmid - (reply: 7)