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Top : New Forum Archives (2009-): : Molecular-Biology
1621. dh5 alpha competent cells? - (reply: 2)
1622. Hypoxia - downregulated HIF-1A - why me??? (reply: 2)
1623. Bioanalyzer not recognising the chip - chip misaligned/manufacturing problem? - (reply: 4)
1624. transient transfection - (reply: 1)
1625. Bad sequence - (reply: 5)
1626. Southern Blot troubleshooting - Please help (reply: 3)
1627. Removing sequencing migration artefacts - (reply: 1)
1628. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
1629. Probes design for southern-blot - need more information for probes design for southern-blot (reply: 3)
1630. finding promoter - (reply: 1)
1631. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
1632. Normalizing RNA concentrations prior to cDNA production - qPCR and making cDNA (reply: 1)
1633. precipitation of secreted protein from growth media - (reply: 1)
1634. EMSA gel problem - (reply: 2)
1635. cloning - (reply: 6)
1636. Generating stable cell line - (reply: 2)
1637. Can't get clean RNA prep with TRIzol - (reply: 2)
1638. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
1639. vectors maps - (reply: 2)
1640. Fusion of reporter genes - (reply: 2)
1641. reuse electroporation cuvette? - (reply: 4)
1642. PCR Master mix - (reply: 1)
1643. Fake positive colony in lamda RED reconbination, why? - (reply: 2)
1644. Cloning from cDNA - the wrong gene?! - (reply: 2)
1645. Ligation - (reply: 3)
1646. circular or linear pDNA for stable clone generation? - (reply: 1)
1647. failure PCR amplification from low GC content gene - (reply: 5)
1648. recombineering for ES cell targeting - retrieval problems (reply: 1)
1649. different PCR primers for real-time and classic PCR - (reply: 3)
1650. Simple gel question - (reply: 7)