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Top : New Forum Archives (2009-): : Molecular-Biology
1621. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
1622. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
1623. Sequence size and insert size - Sequence size has larger base pair than insert size (reply: 2)
1624. QCMD samples - (reply: 5)
1625. competent cells - OD600 reading (reply: 6)
1626. Creating overhangs with PCR - (reply: 4)
1627. RNA: Is this degratation or something else? - (reply: 1)
1628. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1629. Is there any software that can be sued to design degenerate primers - (reply: 1)
1630. Primer Decontamination - (reply: 2)
1631. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1632. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1633. How long is "overnight"? - (reply: 3)
1634. Trizol stability - (reply: 1)
1635. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1636. Tube Openers - (reply: 3)
1637. taq man probes non specific? - (reply: 1)
1638. Help! low efficiency of transfection - (reply: 2)
1639. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1640. Cloning before sequencing- is it necessary??? - (reply: 13)
1641. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1642. Guanadine HCl for plasmid miniprep - (reply: 3)
1643. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1644. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1645. 3īRACE problem - only smear - (reply: 4)
1646. salt for DNA precipitation - DNA purification (reply: 3)
1647. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1648. Not getting plasmid from miniprep - (reply: 7)
1649. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1650. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)