Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1621. Guanadine HCl for plasmid miniprep - (reply: 3)
1622. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1623. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1624. 3īRACE problem - only smear - (reply: 4)
1625. salt for DNA precipitation - DNA purification (reply: 3)
1626. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1627. Not getting plasmid from miniprep - (reply: 7)
1628. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1629. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1630. Is this consider smearing? - (reply: 2)
1631. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1632. grow in broth but not on agar... - after transformation (reply: 2)
1633. How many colonies to screen (colony PCR)? - (reply: 4)
1634. Primer Design - aligning sequences for probe/primer design (reply: 2)
1635. Cloning problem - (reply: 2)
1636. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
1637. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1638. In situ hybridization enigma - (reply: 1)
1639. A cloning nightmare - (reply: 8)
1640. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1641. RNA fragmentation - (reply: 3)
1642. RNA extraction - how many cells do I need at least?? (reply: 4)
1643. RNA extraction protocol for small amounts of cells - (reply: 2)
1644. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
1645. DNA sample contaminated with lipids - (reply: 8)
1646. cloning problem - (reply: 5)
1647. colony PCR inconsistent - (reply: 4)
1648. Newbie to Sequencing Question - (reply: 1)
1649. Problem with gel extraction of DNA. - (reply: 1)
1650. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)