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Top : New Forum Archives (2009-): : Molecular-Biology
1621. Slightly too high 260:280 and 260:230 ratios from gDNA extraction - Should I care? (reply: 2)
1622. Blunt end filling - Blunt end filling (reply: 1)
1623. GHOST in Electrophoresis - (reply: 18)
1624. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
1625. Transformation background problem - high background in negative control (reply: 9)
1626. Akrylamide Gels - (reply: 2)
1627. Does unautoclaved DEPC inhibit northern blotting? - (reply: 2)
1628. Miniprep and sequencing - (reply: 4)
1629. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
1630. Protein TAG for affinity purification - (reply: 1)
1631. Blunt-Cohesive ligation - Should I change the conditions? (reply: 2)
1632. GFP fused proteins - i'm going to cry... (reply: 3)
1633. what's the meaning? - HEAP (reply: 2)
1634. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
1635. Making gels in advance - (reply: 2)
1636. Phenol-chloroform problem - (reply: 1)
1637. Cloning <100 bp fragments into a plasmid - (reply: 7)
1638. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
1639. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
1640. Amount of DNase to use (Qiagen) - (reply: 7)
1641. COMPLETE RNA degradation - (reply: 18)
1642. HpaI blunt end cloning - (reply: 1)
1643. undesired products in multiplex PCR - (reply: 3)
1644. Generate Supercoil Plasmid - it just a thought... (reply: 5)
1645. help with protein localization study using GFP - (reply: 5)
1646. Does a viral gene have introns? - (reply: 4)
1647. Selecting Lethal Mutants? - (reply: 1)
1648. RNA extraction from stained cells - (reply: 2)
1649. agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
1650. No band after digestion - (reply: 4)