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Top : New Forum Archives (2009-): : Molecular-Biology
1621. Blunt-Cohesive ligation - Should I change the conditions? (reply: 2)
1622. GFP fused proteins - i'm going to cry... (reply: 3)
1623. what's the meaning? - HEAP (reply: 2)
1624. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
1625. Making gels in advance - (reply: 2)
1626. Phenol-chloroform problem - (reply: 1)
1627. Cloning <100 bp fragments into a plasmid - (reply: 7)
1628. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
1629. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
1630. Amount of DNase to use (Qiagen) - (reply: 7)
1631. COMPLETE RNA degradation - (reply: 18)
1632. HpaI blunt end cloning - (reply: 1)
1633. undesired products in multiplex PCR - (reply: 3)
1634. Generate Supercoil Plasmid - it just a thought... (reply: 5)
1635. help with protein localization study using GFP - (reply: 5)
1636. Does a viral gene have introns? - (reply: 4)
1637. Selecting Lethal Mutants? - (reply: 1)
1638. RNA extraction from stained cells - (reply: 2)
1639. agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
1640. No band after digestion - (reply: 4)
1641. Problem with ligation - Insert into the "wrong" vector (reply: 11)
1642. finding promoter - (reply: 1)
1643. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
1644. Cloning in pCR2.1 plasmid - (reply: 7)
1645. How long can DNA precipitate at -20? - (reply: 6)
1646. Nucleotides with different font size - (reply: 2)
1647. primer sequence problem - (reply: 2)
1648. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
1649. lambda phage library - (reply: 1)
1650. More colonies fron vector only control? - (reply: 1)