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Top : New Forum Archives (2009-): : Molecular-Biology
1621. Tube Openers - (reply: 3)
1622. taq man probes non specific? - (reply: 1)
1623. Help! low efficiency of transfection - (reply: 2)
1624. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1625. Cloning before sequencing- is it necessary??? - (reply: 13)
1626. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1627. Guanadine HCl for plasmid miniprep - (reply: 3)
1628. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1629. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1630. 3īRACE problem - only smear - (reply: 4)
1631. salt for DNA precipitation - DNA purification (reply: 3)
1632. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1633. Not getting plasmid from miniprep - (reply: 7)
1634. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1635. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1636. Is this consider smearing? - (reply: 2)
1637. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1638. grow in broth but not on agar... - after transformation (reply: 2)
1639. How many colonies to screen (colony PCR)? - (reply: 4)
1640. Primer Design - aligning sequences for probe/primer design (reply: 2)
1641. Cloning problem - (reply: 2)
1642. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
1643. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1644. In situ hybridization enigma - (reply: 1)
1645. A cloning nightmare - (reply: 8)
1646. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1647. RNA fragmentation - (reply: 3)
1648. RNA extraction - how many cells do I need at least?? (reply: 4)
1649. RNA extraction protocol for small amounts of cells - (reply: 2)
1650. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)