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Top : New Forum Archives (2009-): : Molecular-Biology
2611. pIRESneo for mammalian cells transfection - (reply: 4)
2612. H9C2 - cell line - Need clarification (reply: 1)
2613. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
2614. no band in gel - gel electrophoresis (reply: 1)
2615. stratagene site directed mutagenesis - deletion of single base nucleotide (reply: 3)
2616. How to increase A260/280 and A260/230 ratio for microarray experiment? - (reply: 8)
2617. western blot stripping buffer - please recomend a stripping buffer for re-bloting (reply: 2)
2618. T7 and T7lac - (reply: 1)
2619. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
2620. Primer stock confusion - (reply: 6)
2621. Pancreas RNA extraction - improve protocol needed (reply: 2)
2622. Genomic DNA contamination in RNA sample - (reply: 2)
2623. Isolation DNA and protein from a single sample - (reply: 5)
2624. lipofectamine - (reply: 3)
2625. Gel Analysis Software - which to use (reply: 3)
2626. DNase I digestion - check if it worked?? (reply: 4)
2627. Uniform high backgroud in DIG Sounthern Blot - (reply: 2)
2628. elution of dna from gel - (reply: 5)
2629. What is in the Blocking solution for DIG-Southern Blot? - (reply: 2)
2630. In vitro Transcription kit recommendations? - (reply: 5)
2631. GFP reporter gene - (reply: 3)
2632. Possible to lose a pGEM insert ? - (reply: 2)
2633. PROTEIN PRECIPITATION SOLUTION - (reply: 1)
2634. cloning - How to calculate the insert and linear plsmid concentration for ligati (reply: 1)
2635. PFGE gel - Can I reuse pulsed field certified gel for PFGE? (reply: 2)
2636. Validating real time pcr primers - (reply: 2)
2637. How to find catalytic domain using cDNA - (reply: 2)
2638. low plasmid yield from midi preps - (reply: 4)
2639. Measure nucleotides - (reply: 3)
2640. Proteinase K Protection Assay Conditions - (reply: 2)