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Top : New Forum Archives (2009-): : Molecular-Biology
2611. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
2612. no band in gel - gel electrophoresis (reply: 1)
2613. stratagene site directed mutagenesis - deletion of single base nucleotide (reply: 3)
2614. How to increase A260/280 and A260/230 ratio for microarray experiment? - (reply: 8)
2615. western blot stripping buffer - please recomend a stripping buffer for re-bloting (reply: 2)
2616. T7 and T7lac - (reply: 1)
2617. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
2618. Primer stock confusion - (reply: 6)
2619. Pancreas RNA extraction - improve protocol needed (reply: 2)
2620. Genomic DNA contamination in RNA sample - (reply: 2)
2621. Isolation DNA and protein from a single sample - (reply: 5)
2622. lipofectamine - (reply: 3)
2623. Gel Analysis Software - which to use (reply: 3)
2624. DNase I digestion - check if it worked?? (reply: 4)
2625. Uniform high backgroud in DIG Sounthern Blot - (reply: 2)
2626. elution of dna from gel - (reply: 5)
2627. What is in the Blocking solution for DIG-Southern Blot? - (reply: 2)
2628. In vitro Transcription kit recommendations? - (reply: 5)
2629. GFP reporter gene - (reply: 3)
2630. Possible to lose a pGEM insert ? - (reply: 2)
2631. PROTEIN PRECIPITATION SOLUTION - (reply: 1)
2632. cloning - How to calculate the insert and linear plsmid concentration for ligati (reply: 1)
2633. PFGE gel - Can I reuse pulsed field certified gel for PFGE? (reply: 2)
2634. Validating real time pcr primers - (reply: 2)
2635. How to find catalytic domain using cDNA - (reply: 2)
2636. low plasmid yield from midi preps - (reply: 4)
2637. Measure nucleotides - (reply: 3)
2638. Proteinase K Protection Assay Conditions - (reply: 2)
2639. no 18S band on RNA agarose gel - (reply: 1)
2640. b-mercaptoethanol in lysis buffer - (reply: 1)