Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
2611. DNA purification - long fragments wanted - (reply: 1)
2612. want gene knock out protocol - (reply: 2)
2613. Unidentified DNA bands - (reply: 1)
2614. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
2615. Accidentally digested DNA overnight - still OK? - (reply: 4)
2616. Shelf life of ampicillin in LB media? - (reply: 6)
2617. Make construct so it is only thing that can PCR after transfection - (reply: 2)
2618. Negative flouroscence in real time PCR - (reply: 2)
2619. Remove RNA after DNA mini-prep - (reply: 1)
2620. Minimum number of PCR cycles to see a product? - (reply: 6)
2621. Ligation after SalI & XhoI Digestion - (reply: 3)
2622. Rac1 mutants - (reply: 1)
2623. RNA from Sheep and Bovine blood - Isolation of RNA from bovine and ovine whole blood (reply: 3)
2624. decontamination - decontamination for PCR (reply: 6)
2625. Troubleshoot ARMS PCR - (reply: 2)
2626. Transfer of gene from one vector to another vector - (reply: 2)
2627. Transfection of vectors - (reply: 8)
2628. negative control for PCR - (reply: 1)
2629. Proteinase K to improve RNA extraction yield - (reply: 2)
2630. cloudy phase during RNA extraction with Trizol - (reply: 10)
2631. EtBr solution - (reply: 3)
2632. cDNA versus cRNA for a microarray - (reply: 1)
2633. Capillary DNA Sequencing - (reply: 6)
2634. y pGEMT first then pGEX-2T - (reply: 2)
2635. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
2636. Plasmid replication - Plasmid replication (reply: 13)
2637. silica purification - problem with double bands after silica purification (reply: 1)
2638. Non-Radioactive In vitro Kinase Assay - MPF and MAPK specific (reply: 2)
2639. Glycogen carrier 260/230 ratio - (reply: 1)
2640. questions about TOPO cloning - (reply: 7)