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Top : New Forum Archives (2009-): : Molecular-Biology
2521. primer design - (reply: 3)
2522. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
2523. DNA purification - long fragments wanted - (reply: 1)
2524. want gene knock out protocol - (reply: 2)
2525. Unidentified DNA bands - (reply: 1)
2526. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
2527. Accidentally digested DNA overnight - still OK? - (reply: 4)
2528. Shelf life of ampicillin in LB media? - (reply: 6)
2529. Make construct so it is only thing that can PCR after transfection - (reply: 2)
2530. Negative flouroscence in real time PCR - (reply: 2)
2531. Remove RNA after DNA mini-prep - (reply: 1)
2532. Minimum number of PCR cycles to see a product? - (reply: 6)
2533. Ligation after SalI & XhoI Digestion - (reply: 3)
2534. Rac1 mutants - (reply: 1)
2535. RNA from Sheep and Bovine blood - Isolation of RNA from bovine and ovine whole blood (reply: 3)
2536. decontamination - decontamination for PCR (reply: 6)
2537. Troubleshoot ARMS PCR - (reply: 2)
2538. Transfer of gene from one vector to another vector - (reply: 2)
2539. Transfection of vectors - (reply: 8)
2540. negative control for PCR - (reply: 1)
2541. Proteinase K to improve RNA extraction yield - (reply: 2)
2542. cloudy phase during RNA extraction with Trizol - (reply: 10)
2543. EtBr solution - (reply: 3)
2544. cDNA versus cRNA for a microarray - (reply: 1)
2545. Capillary DNA Sequencing - (reply: 6)
2546. y pGEMT first then pGEX-2T - (reply: 2)
2547. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
2548. Plasmid replication - Plasmid replication (reply: 13)
2549. silica purification - problem with double bands after silica purification (reply: 1)
2550. Non-Radioactive In vitro Kinase Assay - MPF and MAPK specific (reply: 2)