Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
2521. problems with DNase1 footprinting - DNA won't enter a denaturing urea polyacrylamide gel (reply: 2)
2522. TOP10F' - Can they be used for regular vector propagation? (reply: 1)
2523. Gel shift with large DNA fragments (>1 kb) - Can I still do EMSA? (reply: 1)
2524. re-amplification in real-time PCR - (reply: 1)
2525. 3'RACE and the polyA tail - why only one band? (reply: 2)
2526. midi-prep - (reply: 5)
2527. Degenerative primers = multiple products? - (reply: 5)
2528. 96 Well minipreps? - (reply: 1)
2529. DNAse activity in room temperature - (reply: 3)
2530. Reverse transcribing organically contaminated RNA - (reply: 2)
2531. Phylogenetic interpretation - (reply: 1)
2532. Do you lose RNA by quick freezing? - (reply: 5)
2533. obatining full nucelotide sequences for genes - where can i find them (reply: 4)
2534. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
2535. Converting RE sites - (reply: 2)
2536. Can DNA be solubilized in hydrochloric acid? - (reply: 2)
2537. house keeping gene in transgenic mice. - (reply: 1)
2538. RNA cleanup methods (WITHOUT USING KITS!!) - protocol for removing phenol contamination (reply: 5)
2539. Detect gene in stable cell line, - (reply: 4)
2540. in vivo expression technology - IVET (reply: 2)
2541. basic math question - Molarity (reply: 2)
2542. EMSA supershift - increased band intensity? - (reply: 1)
2543. Who can explain this? Weird cloning problem.... - (reply: 6)
2544. RNA extraction - Which is which? (reply: 6)
2545. DNase I digestion - how long the fragments are at the end??? (reply: 3)
2546. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
2547. pIRESneo for mammalian cells transfection - (reply: 4)
2548. H9C2 - cell line - Need clarification (reply: 1)
2549. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
2550. no band in gel - gel electrophoresis (reply: 1)