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Top : New Forum Archives (2009-): : Molecular-Biology
2521. Amplifying a gene using a degenerate primer - (reply: 4)
2522. DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
2523. Problem with DNA extraction from 1% agarose Gel - No DNA using Qiagen Qiaek 2 and Spin column kits (reply: 9)
2524. volume of DNA required (PCr product ) in agarose gel - (reply: 11)
2525. Restriction endonuclease - cut only double stand DNA? (reply: 4)
2526. separation of dna fragments in agarose gel electrophoresis - (reply: 7)
2527. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
2528. gene-specific RT-PCR - (reply: 4)
2529. Challenging question for RNA people - (reply: 1)
2530. Plasmid supercoiling affecting PCR? - (reply: 2)
2531. Southern Blot Transfer Buffer Substitutions? - (reply: 1)
2532. cDNA synthesis - need amplified cDNA to do RE digestion (reply: 6)
2533. Genomic material extraction from a vaccine - (reply: 1)
2534. strategy to isolate cDNA and express protein - (reply: 1)
2535. Haemophilus influenzae transposon mutagensis - (reply: 4)
2536. Dry Plant leaf samples for DNA extraction - (reply: 1)
2537. Different beta-actin levels - (reply: 3)
2538. membrane-bound pEGFP as a gift - (reply: 1)
2539. Need help in a test that I will have on Monday - (reply: 1)
2540. RNA extraction using TRIzol - (reply: 19)
2541. dsRNA construct size - (reply: 2)
2542. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
2543. problems with DNase1 footprinting - DNA won't enter a denaturing urea polyacrylamide gel (reply: 2)
2544. TOP10F' - Can they be used for regular vector propagation? (reply: 1)
2545. Gel shift with large DNA fragments (>1 kb) - Can I still do EMSA? (reply: 1)
2546. re-amplification in real-time PCR - (reply: 1)
2547. 3'RACE and the polyA tail - why only one band? (reply: 2)
2548. midi-prep - (reply: 5)
2549. Degenerative primers = multiple products? - (reply: 5)
2550. 96 Well minipreps? - (reply: 1)