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Top : New Forum Archives (2009-): : Molecular-Biology
2521. want gene knock out protocol - (reply: 2)
2522. Unidentified DNA bands - (reply: 1)
2523. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
2524. Accidentally digested DNA overnight - still OK? - (reply: 4)
2525. Shelf life of ampicillin in LB media? - (reply: 6)
2526. Make construct so it is only thing that can PCR after transfection - (reply: 2)
2527. Negative flouroscence in real time PCR - (reply: 2)
2528. Remove RNA after DNA mini-prep - (reply: 1)
2529. Minimum number of PCR cycles to see a product? - (reply: 6)
2530. Ligation after SalI & XhoI Digestion - (reply: 3)
2531. Rac1 mutants - (reply: 1)
2532. RNA from Sheep and Bovine blood - Isolation of RNA from bovine and ovine whole blood (reply: 3)
2533. decontamination - decontamination for PCR (reply: 6)
2534. Troubleshoot ARMS PCR - (reply: 2)
2535. Transfer of gene from one vector to another vector - (reply: 2)
2536. Transfection of vectors - (reply: 8)
2537. negative control for PCR - (reply: 1)
2538. Proteinase K to improve RNA extraction yield - (reply: 2)
2539. cloudy phase during RNA extraction with Trizol - (reply: 10)
2540. EtBr solution - (reply: 3)
2541. cDNA versus cRNA for a microarray - (reply: 1)
2542. Capillary DNA Sequencing - (reply: 6)
2543. y pGEMT first then pGEX-2T - (reply: 2)
2544. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
2545. Plasmid replication - Plasmid replication (reply: 13)
2546. silica purification - problem with double bands after silica purification (reply: 1)
2547. Non-Radioactive In vitro Kinase Assay - MPF and MAPK specific (reply: 2)
2548. Glycogen carrier 260/230 ratio - (reply: 1)
2549. questions about TOPO cloning - (reply: 7)
2550. pcr pdt amplification - (reply: 1)