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Top : New Forum Archives (2009-): : Molecular-Biology
541. Protein extraction and isolectric focusing - (reply: 1)
542. getting strange data in Luciferase-renilla assay - (reply: 2)
543. Homozygote DNA but Heterozygote RNA? - (reply: 2)
544. sacB Counter-Selection to generate double cross-over deletion mutants - (reply: 8)
545. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
546. What is the necessary time for lipofectamin to make complex with pre-miRNA oligo - (reply: 1)
547. what is the ORFeome clone? - (reply: 2)
548. ligation with one ristrection site/enzyme - (reply: 3)
549. No amplification during RT-PCR - (reply: 4)
550. rescue cloning of a transposon mutant - (reply: 2)
551. Dephosphorylation - (reply: 7)
552. study of receptors in cell lines - (reply: 1)
553. Mouse genotyping issues - (reply: 1)
554. In-situ hybridization - (reply: 1)
555. His tag introduction in to gene and primer design - (reply: 2)
556. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
557. Principle of silica membrane binding of nucleic acid - (reply: 6)
558. genogroup and genotype - (reply: 2)
559. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
560. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
561. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
562. Beta alanine alternative? - (reply: 2)
563. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
564. lncRNA full-sequence or partial sequence - (reply: 2)
565. Cloning at 6 kb gene - (reply: 3)
566. Trouble with DNA purity (A260/A280) - (reply: 3)
567. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
568. Luciferase assay - extreme variation in RLU - (reply: 1)
569. site-directed mutagenesis - (reply: 20)
570. qPCR detection - (reply: 2)