Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
541. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
542. Beta alanine alternative? - (reply: 2)
543. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
544. lncRNA full-sequence or partial sequence - (reply: 2)
545. Cloning at 6 kb gene - (reply: 3)
546. Trouble with DNA purity (A260/A280) - (reply: 3)
547. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
548. Luciferase assay - extreme variation in RLU - (reply: 1)
549. site-directed mutagenesis - (reply: 20)
550. qPCR detection - (reply: 2)
551. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
552. TCA precipitation - (reply: 1)
553. SOC vs TB in transformations - (reply: 1)
554. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
555. Single RE site cloning - (reply: 3)
556. PCR product running on agarose gel - (reply: 32)
557. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
558. PCR amplification with high fidelity enzyme - (reply: 1)
559. Transformation problem - (reply: 5)
560. finding gene sequence to delete - (reply: 4)
561. Blue fluorescent color of agarose gel in UV - (reply: 7)
562. heterologous gene integration into e.coli genome - (reply: 1)
563. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
564. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
565. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
566. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
567. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
568. Continuous growth of E. coli on AMP LB plate - (reply: 2)
569. Plasmid compatibly groups - (reply: 5)
570. too much DNA on agarose gel - (reply: 10)