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Top : New Forum Archives (2009-): : Molecular-Biology
541. Cloning at 6 kb gene - (reply: 3)
542. Trouble with DNA purity (A260/A280) - (reply: 3)
543. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
544. Luciferase assay - extreme variation in RLU - (reply: 1)
545. site-directed mutagenesis - (reply: 20)
546. qPCR detection - (reply: 2)
547. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
548. TCA precipitation - (reply: 1)
549. SOC vs TB in transformations - (reply: 1)
550. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
551. Single RE site cloning - (reply: 3)
552. PCR product running on agarose gel - (reply: 32)
553. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
554. PCR amplification with high fidelity enzyme - (reply: 1)
555. Transformation problem - (reply: 5)
556. finding gene sequence to delete - (reply: 4)
557. Blue fluorescent color of agarose gel in UV - (reply: 7)
558. heterologous gene integration into e.coli genome - (reply: 1)
559. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
560. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
561. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
562. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
563. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
564. Continuous growth of E. coli on AMP LB plate - (reply: 2)
565. Plasmid compatibly groups - (reply: 5)
566. too much DNA on agarose gel - (reply: 10)
567. What are the "mammalian vectors N1 and C1"? - (reply: 1)
568. Life before BLAST etc? Help needed... - (reply: 1)
569. How base pairs upstream of gene to include for the promoter? - (reply: 3)
570. Neomycin resistance sequence - (reply: 1)