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Top : New Forum Archives (2009-): : Molecular-Biology
541. site-directed mutagenesis - (reply: 20)
542. qPCR detection - (reply: 2)
543. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
544. TCA precipitation - (reply: 1)
545. SOC vs TB in transformations - (reply: 1)
546. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
547. Single RE site cloning - (reply: 3)
548. PCR product running on agarose gel - (reply: 32)
549. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
550. PCR amplification with high fidelity enzyme - (reply: 1)
551. Transformation problem - (reply: 5)
552. finding gene sequence to delete - (reply: 4)
553. Blue fluorescent color of agarose gel in UV - (reply: 7)
554. heterologous gene integration into e.coli genome - (reply: 1)
555. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
556. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
557. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
558. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
559. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
560. Continuous growth of E. coli on AMP LB plate - (reply: 2)
561. Plasmid compatibly groups - (reply: 5)
562. too much DNA on agarose gel - (reply: 10)
563. What are the "mammalian vectors N1 and C1"? - (reply: 1)
564. Life before BLAST etc? Help needed... - (reply: 1)
565. How base pairs upstream of gene to include for the promoter? - (reply: 3)
566. Neomycin resistance sequence - (reply: 1)
567. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
568. polyacrylamide RNA gel - (reply: 4)
569. Making a standard curve from bacterial suspensions - (reply: 1)
570. Is my RNA degraded? - (reply: 7)