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Top : New Forum Archives (2009-): : Molecular-Biology
541. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
542. Principle of silica membrane binding of nucleic acid - (reply: 6)
543. genogroup and genotype - (reply: 2)
544. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
545. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
546. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
547. Beta alanine alternative? - (reply: 2)
548. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
549. lncRNA full-sequence or partial sequence - (reply: 2)
550. Cloning at 6 kb gene - (reply: 3)
551. Trouble with DNA purity (A260/A280) - (reply: 3)
552. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
553. Luciferase assay - extreme variation in RLU - (reply: 1)
554. site-directed mutagenesis - (reply: 20)
555. qPCR detection - (reply: 2)
556. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
557. TCA precipitation - (reply: 1)
558. SOC vs TB in transformations - (reply: 1)
559. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
560. Single RE site cloning - (reply: 3)
561. PCR product running on agarose gel - (reply: 32)
562. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
563. PCR amplification with high fidelity enzyme - (reply: 1)
564. Transformation problem - (reply: 5)
565. finding gene sequence to delete - (reply: 4)
566. Blue fluorescent color of agarose gel in UV - (reply: 7)
567. heterologous gene integration into e.coli genome - (reply: 1)
568. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
569. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
570. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)