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Top : New Forum Archives (2009-): : Molecular-Biology
1501. DNA ext from paraffin-embedded tissue ... a dilemma ! - FFPE tissue refuse to lyse strange whitish clumps formed!! (reply: 1)
1502. Glycine linker - (reply: 2)
1503. Why does the amount of plamids transformed affect colony count? - (reply: 2)
1504. How to obatin intron sequence from cDNA sequence? - (reply: 1)
1505. genomic DNA extraction - why there appears a smear on the gel photo (reply: 11)
1506. Colony Pcr - primers - (reply: 5)
1507. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
1508. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
1509. Sequence size and insert size - Sequence size has larger base pair than insert size (reply: 2)
1510. QCMD samples - (reply: 5)
1511. competent cells - OD600 reading (reply: 6)
1512. Creating overhangs with PCR - (reply: 4)
1513. RNA: Is this degratation or something else? - (reply: 1)
1514. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1515. Is there any software that can be sued to design degenerate primers - (reply: 1)
1516. Primer Decontamination - (reply: 2)
1517. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1518. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1519. How long is "overnight"? - (reply: 3)
1520. Trizol stability - (reply: 1)
1521. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1522. Tube Openers - (reply: 3)
1523. taq man probes non specific? - (reply: 1)
1524. Help! low efficiency of transfection - (reply: 2)
1525. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1526. Cloning before sequencing- is it necessary??? - (reply: 13)
1527. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1528. Guanadine HCl for plasmid miniprep - (reply: 3)
1529. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1530. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)