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Top : New Forum Archives (2009-): : Molecular-Biology
1501. How long is "overnight"? - (reply: 3)
1502. Trizol stability - (reply: 1)
1503. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1504. Tube Openers - (reply: 3)
1505. taq man probes non specific? - (reply: 1)
1506. Help! low efficiency of transfection - (reply: 2)
1507. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1508. Cloning before sequencing- is it necessary??? - (reply: 13)
1509. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1510. Guanadine HCl for plasmid miniprep - (reply: 3)
1511. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1512. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1513. 3īRACE problem - only smear - (reply: 4)
1514. salt for DNA precipitation - DNA purification (reply: 3)
1515. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1516. Not getting plasmid from miniprep - (reply: 7)
1517. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1518. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1519. Is this consider smearing? - (reply: 2)
1520. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1521. grow in broth but not on agar... - after transformation (reply: 2)
1522. How many colonies to screen (colony PCR)? - (reply: 4)
1523. Primer Design - aligning sequences for probe/primer design (reply: 2)
1524. Cloning problem - (reply: 2)
1525. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
1526. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1527. In situ hybridization enigma - (reply: 1)
1528. A cloning nightmare - (reply: 8)
1529. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1530. RNA fragmentation - (reply: 3)