Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1681. Transformation background problem - high background in negative control (reply: 9)
1682. Akrylamide Gels - (reply: 2)
1683. Does unautoclaved DEPC inhibit northern blotting? - (reply: 2)
1684. Miniprep and sequencing - (reply: 4)
1685. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
1686. Protein TAG for affinity purification - (reply: 1)
1687. Blunt-Cohesive ligation - Should I change the conditions? (reply: 2)
1688. GFP fused proteins - i'm going to cry... (reply: 3)
1689. what's the meaning? - HEAP (reply: 2)
1690. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
1691. Making gels in advance - (reply: 2)
1692. Phenol-chloroform problem - (reply: 1)
1693. Cloning <100 bp fragments into a plasmid - (reply: 7)
1694. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
1695. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
1696. Amount of DNase to use (Qiagen) - (reply: 7)
1697. COMPLETE RNA degradation - (reply: 18)
1698. HpaI blunt end cloning - (reply: 1)
1699. undesired products in multiplex PCR - (reply: 3)
1700. Generate Supercoil Plasmid - it just a thought... (reply: 5)
1701. help with protein localization study using GFP - (reply: 5)
1702. Does a viral gene have introns? - (reply: 4)
1703. Selecting Lethal Mutants? - (reply: 1)
1704. RNA extraction from stained cells - (reply: 2)
1705. agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
1706. No band after digestion - (reply: 4)
1707. Problem with ligation - Insert into the "wrong" vector (reply: 11)
1708. finding promoter - (reply: 1)
1709. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
1710. Cloning in pCR2.1 plasmid - (reply: 7)