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1681. Protein TAG for affinity purification - (reply: 1)
1682. Blunt-Cohesive ligation - Should I change the conditions? (reply: 2)
1683. GFP fused proteins - i'm going to cry... (reply: 3)
1684. what's the meaning? - HEAP (reply: 2)
1685. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
1686. Making gels in advance - (reply: 2)
1687. Phenol-chloroform problem - (reply: 1)
1688. Cloning <100 bp fragments into a plasmid - (reply: 7)
1689. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
1690. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
1691. Amount of DNase to use (Qiagen) - (reply: 7)
1692. COMPLETE RNA degradation - (reply: 18)
1693. HpaI blunt end cloning - (reply: 1)
1694. undesired products in multiplex PCR - (reply: 3)
1695. Generate Supercoil Plasmid - it just a thought... (reply: 5)
1696. help with protein localization study using GFP - (reply: 5)
1697. Does a viral gene have introns? - (reply: 4)
1698. Selecting Lethal Mutants? - (reply: 1)
1699. RNA extraction from stained cells - (reply: 2)
1700. agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
1701. No band after digestion - (reply: 4)
1702. Problem with ligation - Insert into the "wrong" vector (reply: 11)
1703. finding promoter - (reply: 1)
1704. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
1705. Cloning in pCR2.1 plasmid - (reply: 7)
1706. How long can DNA precipitate at -20? - (reply: 6)
1707. Nucleotides with different font size - (reply: 2)
1708. primer sequence problem - (reply: 2)
1709. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
1710. lambda phage library - (reply: 1)