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Top : New Forum Archives (2009-): : Molecular-Biology
2731. Freeze thawing RNA - (reply: 4)
2732. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
2733. Precipitation in NaOH on shelf - (reply: 3)
2734. long PCR primer - (reply: 7)
2735. stable 3t3l1 transfection:serious help needed!!! - (reply: 10)
2736. methods to detect EGFP expression ?! - we have a big problem! (reply: 10)
2737. mutation study - DNA or mRNA sequence (reply: 1)
2738. how to remove a restricted endonuclease site - (reply: 3)
2739. substractive hybridization - (reply: 2)
2740. sequencing problem after pcr - (reply: 4)
2741. Amplifying plasmid - non specific binding of primer - (reply: 3)
2742. DNA folding to form the loop - (reply: 2)
2743. primer design - (reply: 3)
2744. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
2745. DNA purification - long fragments wanted - (reply: 1)
2746. want gene knock out protocol - (reply: 2)
2747. Unidentified DNA bands - (reply: 1)
2748. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
2749. Accidentally digested DNA overnight - still OK? - (reply: 4)
2750. Shelf life of ampicillin in LB media? - (reply: 6)
2751. Make construct so it is only thing that can PCR after transfection - (reply: 2)
2752. Negative flouroscence in real time PCR - (reply: 2)
2753. Remove RNA after DNA mini-prep - (reply: 1)
2754. Minimum number of PCR cycles to see a product? - (reply: 6)
2755. Ligation after SalI & XhoI Digestion - (reply: 3)
2756. Rac1 mutants - (reply: 1)
2757. RNA from Sheep and Bovine blood - Isolation of RNA from bovine and ovine whole blood (reply: 3)
2758. decontamination - decontamination for PCR (reply: 6)
2759. Troubleshoot ARMS PCR - (reply: 2)
2760. Transfer of gene from one vector to another vector - (reply: 2)