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Top : New Forum Archives (2009-): : Molecular-Biology
2731. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
2732. Accidentally digested DNA overnight - still OK? - (reply: 4)
2733. Shelf life of ampicillin in LB media? - (reply: 6)
2734. Make construct so it is only thing that can PCR after transfection - (reply: 2)
2735. Negative flouroscence in real time PCR - (reply: 2)
2736. Remove RNA after DNA mini-prep - (reply: 1)
2737. Minimum number of PCR cycles to see a product? - (reply: 6)
2738. Ligation after SalI & XhoI Digestion - (reply: 3)
2739. Rac1 mutants - (reply: 1)
2740. RNA from Sheep and Bovine blood - Isolation of RNA from bovine and ovine whole blood (reply: 3)
2741. decontamination - decontamination for PCR (reply: 6)
2742. Troubleshoot ARMS PCR - (reply: 2)
2743. Transfer of gene from one vector to another vector - (reply: 2)
2744. Transfection of vectors - (reply: 8)
2745. negative control for PCR - (reply: 1)
2746. Proteinase K to improve RNA extraction yield - (reply: 2)
2747. cloudy phase during RNA extraction with Trizol - (reply: 10)
2748. EtBr solution - (reply: 3)
2749. cDNA versus cRNA for a microarray - (reply: 1)
2750. Capillary DNA Sequencing - (reply: 6)
2751. y pGEMT first then pGEX-2T - (reply: 2)
2752. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
2753. Plasmid replication - Plasmid replication (reply: 13)
2754. silica purification - problem with double bands after silica purification (reply: 1)
2755. Non-Radioactive In vitro Kinase Assay - MPF and MAPK specific (reply: 2)
2756. Glycogen carrier 260/230 ratio - (reply: 1)
2757. questions about TOPO cloning - (reply: 7)
2758. pcr pdt amplification - (reply: 1)
2759. Promoter analysis - Finding potential binding and receptor site (reply: 4)
2760. Plasmid extraction sans commercial kits - (reply: 3)