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Top : New Forum Archives (2009-): : Molecular-Biology
1831. number of molecules in PCR - (reply: 3)
1832. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
1833. EMSA - how to make long probes ? (reply: 4)
1834. point mutation when transformed in BL21 - (reply: 2)
1835. Primer dilution - PCR primer dilution (reply: 2)
1836. DNA quantification: NanoDrop vs. Picogreen - measurement of ChIP Input DNA (reply: 9)
1837. PCR reagent autoclave - (reply: 2)
1838. weird site directed mutagenesis products - (reply: 3)
1839. extraction of viral rna from plant tissue - (reply: 1)
1840. ampicillin for LB agar - (reply: 2)
1841. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
1842. PCR not working - overhangs too long? (reply: 3)
1843. What melting temperature to use - (reply: 1)
1844. PFGE Current? - (reply: 1)
1845. Plasmid frameshift problem after clonning - (reply: 2)
1846. Nde1-BamH1 makes me sad - high background despite DIFFERENT methods (reply: 34)
1847. TA cloning necessary? - (reply: 2)
1848. Is DNA an acid because of phosphate group - (reply: 2)
1849. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
1850. colony formation assay - how many cells to seed (reply: 2)
1851. Conversion of primer unit - ug/ul to uM (reply: 4)
1852. mutagenesis by PCR or just use a kit - (reply: 5)
1853. Drying RNA in Speedvac - (reply: 1)
1854. cDNA for qPCR - Help with basics please (reply: 6)
1855. plasmid extraction kit solution composition - wash buffer (reply: 2)
1856. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
1857. Primer Trouble Shooting - (reply: 5)
1858. Proven Primers Stopped working & now leave smear! - (reply: 2)
1859. Expired chemicals - 0.5M EDTA, can be used again after autoclaved? - (reply: 4)
1860. tri reagent not compatible with plastic? - (reply: 1)