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Top : New Forum Archives (2009-): : Molecular-Biology
1831. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
1832. PCR not working - overhangs too long? (reply: 3)
1833. What melting temperature to use - (reply: 1)
1834. PFGE Current? - (reply: 1)
1835. Plasmid frameshift problem after clonning - (reply: 2)
1836. Nde1-BamH1 makes me sad - high background despite DIFFERENT methods (reply: 34)
1837. TA cloning necessary? - (reply: 2)
1838. Is DNA an acid because of phosphate group - (reply: 2)
1839. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
1840. colony formation assay - how many cells to seed (reply: 2)
1841. Conversion of primer unit - ug/ul to uM (reply: 4)
1842. mutagenesis by PCR or just use a kit - (reply: 5)
1843. Drying RNA in Speedvac - (reply: 1)
1844. cDNA for qPCR - Help with basics please (reply: 6)
1845. plasmid extraction kit solution composition - wash buffer (reply: 2)
1846. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
1847. Primer Trouble Shooting - (reply: 5)
1848. Proven Primers Stopped working & now leave smear! - (reply: 2)
1849. Expired chemicals - 0.5M EDTA, can be used again after autoclaved? - (reply: 4)
1850. tri reagent not compatible with plastic? - (reply: 1)
1851. DNA Extraction - (reply: 2)
1852. ligation problem - (reply: 6)
1853. TRIzol v. TRI reagent - (reply: 2)
1854. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
1855. Isolation of DNA from Scat - (reply: 2)
1856. How long will cellular resources last - (reply: 1)
1857. PFGE conditions - (reply: 2)
1858. genomic DNA isolation - (reply: 4)
1859. Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
1860. How to find gene homologues? - (reply: 1)