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Top : New Forum Archives (2009-): : Molecular-Biology
1351. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1352. RNA extraction - (reply: 3)
1353. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1354. finding exons for a gene, having the coding sequence only - (reply: 4)
1355. colonies without insert!!! - molecular biology (reply: 1)
1356. Effect of phosphoric acid on a kinase assay - (reply: 2)
1357. Proteinase K stock solution - (reply: 3)
1358. Total RNA only produce 1 band in gel - (reply: 2)
1359. Advice on amount of starting template for subcloning - (reply: 3)
1360. New to Gene cloning - Plasmid vector preparation (reply: 2)
1361. PCR for MULTIPLE mutagenesis - (reply: 4)
1362. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
1363. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
1364. Induction strength : IPTG vs lactose - which one is stronger? (reply: 2)
1365. EMSA - probe disappears with nuclear extract - probe works without nuclear extract... (reply: 1)
1366. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
1367. Digested PCR product migrate slower than uncut - (reply: 8)
1368. PCR question - (reply: 2)
1369. cloning a 34kb insert - please suggest good vectors (reply: 1)
1370. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
1371. Do you know good sequencing companies? - Sanger sequencing (reply: 15)
1372. RNA extraction troubleshooting: low 260/230 - (reply: 1)
1373. Illumina sample prep for Multiplexed sequencing... - How does it work? (reply: 3)
1374. RNA in native gel - (reply: 3)
1375. Comment of this cloning strategy - (reply: 3)
1376. Problems with transfection in human cells - (reply: 3)
1377. RNA extraction from FTA classic - (reply: 1)
1378. mCherry propagation - (reply: 1)
1379. ClustalW question - on multiple alignment of DNA sequences (reply: 1)
1380. My DNA pellet doesn't dissolve - Overdried?? (reply: 3)