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Top : New Forum Archives (2009-): : Molecular-Biology
1351. purification of PCR product for cloning in a vector - (reply: 3)
1352. elimination of Protein interference from DNA - for RT-PCR (reply: 2)
1353. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
1354. Adapter ligation problem - (reply: 5)
1355. Primer design with a tag - (reply: 4)
1356. Lithium electrophoresis buffer - Why does it not work? (reply: 2)
1357. Weird migration of double digested plasmid - (reply: 4)
1358. Run agarose/formaldehyde gel for 5 mins before loading samples? Why? - (reply: 1)
1359. Myc tags - (reply: 1)
1360. MOPS: How do you sterlise yours? - (reply: 2)
1361. Which gel concentration - (reply: 4)
1362. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
1363. PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
1364. MINI Dialysis Units - below 2K MWCO? (reply: 1)
1365. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
1366. Rotten Fish Smell in TAE Buffer - Stinky TAE (reply: 3)
1367. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
1368. Combining forward and reverse primer gives different size on gel - (reply: 2)
1369. Help for co-transfection of proteins and plasmid - (reply: 1)
1370. PCR of complement genomic DNA - (reply: 3)
1371. RNAi is somatic and germline cells - (reply: 3)
1372. subcloning into bacterial expression vector - (reply: 1)
1373. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
1374. cloning into F1 ori of expression vector??? - (reply: 1)
1375. primers for fungal identification - (reply: 9)
1376. protein overexpression in HeLa cells - (reply: 1)
1377. TBE Acrylamide/Formamide gels running slowly - (reply: 4)
1378. Sequencing promoter regions - (reply: 6)
1379. PCR for long and repetitive region from genomic DNA - (reply: 4)
1380. dna isolation - dna isolation (reply: 1)