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Top : New Forum Archives (2009-): : Molecular-Biology
1441. Colony Pcr - primers - (reply: 5)
1442. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
1443. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
1444. Sequence size and insert size - Sequence size has larger base pair than insert size (reply: 2)
1445. QCMD samples - (reply: 5)
1446. competent cells - OD600 reading (reply: 6)
1447. Creating overhangs with PCR - (reply: 4)
1448. RNA: Is this degratation or something else? - (reply: 1)
1449. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1450. Is there any software that can be sued to design degenerate primers - (reply: 1)
1451. Primer Decontamination - (reply: 2)
1452. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1453. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1454. How long is "overnight"? - (reply: 3)
1455. Trizol stability - (reply: 1)
1456. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1457. Tube Openers - (reply: 3)
1458. taq man probes non specific? - (reply: 1)
1459. Help! low efficiency of transfection - (reply: 2)
1460. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1461. Cloning before sequencing- is it necessary??? - (reply: 13)
1462. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1463. Guanadine HCl for plasmid miniprep - (reply: 3)
1464. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1465. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1466. 3īRACE problem - only smear - (reply: 4)
1467. salt for DNA precipitation - DNA purification (reply: 3)
1468. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1469. Not getting plasmid from miniprep - (reply: 7)
1470. DNA sequencing question help - make up sequencing mastermix (reply: 1)