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Top : New Forum Archives (2009-): : Molecular-Biology
1441. How many colonies to screen (colony PCR)? - (reply: 4)
1442. Primer Design - aligning sequences for probe/primer design (reply: 2)
1443. Cloning problem - (reply: 2)
1444. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
1445. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1446. In situ hybridization enigma - (reply: 1)
1447. A cloning nightmare - (reply: 8)
1448. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1449. RNA fragmentation - (reply: 3)
1450. RNA extraction - how many cells do I need at least?? (reply: 4)
1451. RNA extraction protocol for small amounts of cells - (reply: 2)
1452. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
1453. DNA sample contaminated with lipids - (reply: 8)
1454. cloning problem - (reply: 5)
1455. colony PCR inconsistent - (reply: 4)
1456. Newbie to Sequencing Question - (reply: 1)
1457. Problem with gel extraction of DNA. - (reply: 1)
1458. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
1459. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
1460. genomic DNA isolation - (reply: 1)
1461. E. coli not growing in liquid cultures :( - (reply: 4)
1462. Tricine SDS-PAGE for plant mitochondria - Membrane proteomics (reply: 1)
1463. DNA product almost 1500 bp - (reply: 1)
1464. Slightly too high 260:280 and 260:230 ratios from gDNA extraction - Should I care? (reply: 2)
1465. Blunt end filling - Blunt end filling (reply: 1)
1466. GHOST in Electrophoresis - (reply: 18)
1467. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
1468. Transformation background problem - high background in negative control (reply: 9)
1469. Akrylamide Gels - (reply: 2)
1470. Does unautoclaved DEPC inhibit northern blotting? - (reply: 2)