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1471. competent cells - OD600 reading (reply: 6)
1472. Creating overhangs with PCR - (reply: 4)
1473. RNA: Is this degratation or something else? - (reply: 1)
1474. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1475. Is there any software that can be sued to design degenerate primers - (reply: 1)
1476. Primer Decontamination - (reply: 2)
1477. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1478. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1479. How long is "overnight"? - (reply: 3)
1480. Trizol stability - (reply: 1)
1481. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1482. Tube Openers - (reply: 3)
1483. taq man probes non specific? - (reply: 1)
1484. Help! low efficiency of transfection - (reply: 2)
1485. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1486. Cloning before sequencing- is it necessary??? - (reply: 13)
1487. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1488. Guanadine HCl for plasmid miniprep - (reply: 3)
1489. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1490. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1491. 3īRACE problem - only smear - (reply: 4)
1492. salt for DNA precipitation - DNA purification (reply: 3)
1493. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1494. Not getting plasmid from miniprep - (reply: 7)
1495. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1496. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1497. Is this consider smearing? - (reply: 2)
1498. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1499. grow in broth but not on agar... - after transformation (reply: 2)
1500. How many colonies to screen (colony PCR)? - (reply: 4)
1472. Creating overhangs with PCR - (reply: 4)
1473. RNA: Is this degratation or something else? - (reply: 1)
1474. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1475. Is there any software that can be sued to design degenerate primers - (reply: 1)
1476. Primer Decontamination - (reply: 2)
1477. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1478. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1479. How long is "overnight"? - (reply: 3)
1480. Trizol stability - (reply: 1)
1481. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1482. Tube Openers - (reply: 3)
1483. taq man probes non specific? - (reply: 1)
1484. Help! low efficiency of transfection - (reply: 2)
1485. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1486. Cloning before sequencing- is it necessary??? - (reply: 13)
1487. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1488. Guanadine HCl for plasmid miniprep - (reply: 3)
1489. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1490. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1491. 3īRACE problem - only smear - (reply: 4)
1492. salt for DNA precipitation - DNA purification (reply: 3)
1493. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1494. Not getting plasmid from miniprep - (reply: 7)
1495. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1496. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1497. Is this consider smearing? - (reply: 2)
1498. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1499. grow in broth but not on agar... - after transformation (reply: 2)
1500. How many colonies to screen (colony PCR)? - (reply: 4)