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Top : New Forum Archives (2009-): : Molecular-Biology
1471. Trizol stability - (reply: 1)
1472. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1473. Tube Openers - (reply: 3)
1474. taq man probes non specific? - (reply: 1)
1475. Help! low efficiency of transfection - (reply: 2)
1476. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1477. Cloning before sequencing- is it necessary??? - (reply: 13)
1478. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1479. Guanadine HCl for plasmid miniprep - (reply: 3)
1480. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1481. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1482. 3īRACE problem - only smear - (reply: 4)
1483. salt for DNA precipitation - DNA purification (reply: 3)
1484. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1485. Not getting plasmid from miniprep - (reply: 7)
1486. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1487. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1488. Is this consider smearing? - (reply: 2)
1489. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1490. grow in broth but not on agar... - after transformation (reply: 2)
1491. How many colonies to screen (colony PCR)? - (reply: 4)
1492. Primer Design - aligning sequences for probe/primer design (reply: 2)
1493. Cloning problem - (reply: 2)
1494. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
1495. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1496. In situ hybridization enigma - (reply: 1)
1497. A cloning nightmare - (reply: 8)
1498. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1499. RNA fragmentation - (reply: 3)
1500. RNA extraction - how many cells do I need at least?? (reply: 4)