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Top : New Forum Archives (2009-): : Molecular-Biology
1471. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1472. Is this consider smearing? - (reply: 2)
1473. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1474. grow in broth but not on agar... - after transformation (reply: 2)
1475. How many colonies to screen (colony PCR)? - (reply: 4)
1476. Primer Design - aligning sequences for probe/primer design (reply: 2)
1477. Cloning problem - (reply: 2)
1478. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
1479. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1480. In situ hybridization enigma - (reply: 1)
1481. A cloning nightmare - (reply: 8)
1482. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1483. RNA fragmentation - (reply: 3)
1484. RNA extraction - how many cells do I need at least?? (reply: 4)
1485. RNA extraction protocol for small amounts of cells - (reply: 2)
1486. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
1487. DNA sample contaminated with lipids - (reply: 8)
1488. cloning problem - (reply: 5)
1489. colony PCR inconsistent - (reply: 4)
1490. Newbie to Sequencing Question - (reply: 1)
1491. Problem with gel extraction of DNA. - (reply: 1)
1492. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
1493. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
1494. genomic DNA isolation - (reply: 1)
1495. E. coli not growing in liquid cultures :( - (reply: 4)
1496. Tricine SDS-PAGE for plant mitochondria - Membrane proteomics (reply: 1)
1497. DNA product almost 1500 bp - (reply: 1)
1498. Slightly too high 260:280 and 260:230 ratios from gDNA extraction - Should I care? (reply: 2)
1499. Blunt end filling - Blunt end filling (reply: 1)
1500. GHOST in Electrophoresis - (reply: 18)